Marco, CPTAC-3472 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Marco	N/A

Sequence Length (AA)	Molecular Weight (Da)
518	52730

Protein Name
Macrophage receptor MARCO

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGSKELLKEE	DFLGSTEDRA	DFDQAMFPVM	ETFEINDPVP	KKRNGGTFCM
60	70	80	90	100
AVMAIHLILL	TAGTALLLIQ	VLNLQEQLQM	LEMCCGNGSL	AIEDKPFFSL
110	120	130	140	150
QWAPKTHLVP	RAQGLQALQA	QLSWVHTSQE	QLRQQFNNLT	QNPELFQIKG
160	170	180	190	200
ERGSPGPKGA	PGAPGIPGLP	GPAAEKGEKG	AAGRDGTPGV	QGPQGPPGSK
210	220	230	240	250
GEAGLQGLTG	APGKQGATGA	PGPRGEKGSK	GDIGLTGPKG	EHGTKGDKGD
260	270	280	290	300
LGLPGNKGDM	GMKGDTGPMG	SPGAQGGKGD	AGKPGLPGLA	GSPGVKGDQG
310	320	330	340	350
KPGVQGVPGP	QGAPGLSGAK	GEPGRTGLPG	PAGPPGIAGN	PGIAGVKGSK
360	370	380	390	400
GDTGIQGQKG	TKGESGVPGL	VGRKGDTGSP	GLAGPKGEPG	RVGQKGDPGM
410	420	430	440	450
KGSSGQQGQK	GEKGQKGESF	QRVRIMGGTN	RGRAEVYYNN	EWGTICDDDW
460	470	480	490	500
DNNDATVFCR	MLGYSRGRAL	SSYGGGSGNI	WLDNVNCRGT	ENSLWDCSKN
510	518
SWGNHNCVHN	EDAGVECS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3472 Collapse assay details

Data source: Panorama

Official Gene Symbol: Marco
Peptide Sequence: GESGVPGLVGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 363
Peptide End: 373

CPTAC ID: non-CPTAC-3472
Peptide Molecular Mass: 1,026.5458
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	21.9	9.3	8.9	23.9	9.9	11.6	32.4	13.6	14.6	15	15	15
y6 (1+)	4.9	3.6	6.8	6	5.4	7.8	7.7	6.5	10.3	15	15	15
y5 (1+)	16.3	16.3	6.3	19.1	17.8	10.5	25.1	24.1	12.2	15	15	15
y6 (2+)	17.4	7.5	6.9	17.9	6.8	6	25	10.1	9.1	15	15	15
b5 (1+)	8	5.6	6.8	8.6	7.5	4.8	11.7	9.4	8.3	15	15	15
sum	4.2	2.1	4.8	5.2	4.6	5.7	6.7	5.1	7.5	15	15	15

Marco

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3472 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Marco

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3472 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments