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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Serpina3a N/A
Sequence Length (AA) Molecular Weight (Da)
422 47772
Protein Name
Serine protease inhibitor A3A
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAFIAALGLL MVGICPAVTY WATADGQLGG HTAVQKDRDH EIQLDSVTLA
60 70 80 90 100
SINTDFAFSL YKKLALENPH KNIVFSPLSI SAALALMSLG AKGNTLEEIL
110 120 130 140 150
EGLKFNLPET PEADIHQNFG HLLQMLIQPE NQVQINAGNA LFIDKHLQIL
160 170 180 190 200
TEFKEKARAL YKAEAFTTDF QRPREATKLI NDYVRKQTQG KIKELVSDLH
210 220 230 240 250
RNTSMALVNF LNFQGFWNVT FDPEDTFLGN FTLDRKRTVN VPMMKTEELT
260 270 280 290 300
TNYFRDEEMQ STVMELNYIG NASFLFILPD QGRIQHVEDS LQPQSLRKWR
310 320 330 340 350
KSLRPRMLDE LSLPKFSLSQ DYNLNDILPE LGIKEVFSTQ ADLSGITGAK
360 370 380 390 400
NIRVSQMIHQ AALDVTETHT EADVITIARY NFQSAKIKAK IVKVDREFLY
410 420 422
LILDPMFKSI SVMGKVINPL TN

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3500 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serpina3a
Peptide Sequence
IQHVEDSLQPQSLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
284
Peptide End
297
CPTAC ID
non-CPTAC-3500
Peptide Molecular Mass
1,648.8533
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b9 (2+) 6 2.8 3.5 6.7 3.8 3.6 9 4.7 5 15 15 15
b8 (2+) 15.6 7.6 5.6 15.5 10.5 6.7 22 13 8.7 15 15 15
b7 (1+) 10.1 2.1 6.4 11.5 7.2 8.4 15.3 7.5 10.6 15 15 15
y5 (2+) 9.3 3.3 3.5 12.5 3.9 4.4 15.6 5.1 5.6 15 15 15
y5 (1+) 28.2 3 3.5 39.8 5.4 3.9 48.8 6.2 5.2 15 15 15
sum 15.9 2 1.5 20.7 3.8 2.2 26.1 4.3 2.7 15 15 15


Additional Resources and Comments