Serpina3a, CPTAC-3500 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Serpina3a	N/A

Sequence Length (AA)	Molecular Weight (Da)
422	47772

Protein Name
Serine protease inhibitor A3A

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAFIAALGLL	MVGICPAVTY	WATADGQLGG	HTAVQKDRDH	EIQLDSVTLA
60	70	80	90	100
SINTDFAFSL	YKKLALENPH	KNIVFSPLSI	SAALALMSLG	AKGNTLEEIL
110	120	130	140	150
EGLKFNLPET	PEADIHQNFG	HLLQMLIQPE	NQVQINAGNA	LFIDKHLQIL
160	170	180	190	200
TEFKEKARAL	YKAEAFTTDF	QRPREATKLI	NDYVRKQTQG	KIKELVSDLH
210	220	230	240	250
RNTSMALVNF	LNFQGFWNVT	FDPEDTFLGN	FTLDRKRTVN	VPMMKTEELT
260	270	280	290	300
TNYFRDEEMQ	STVMELNYIG	NASFLFILPD	QGRIQHVEDS	LQPQSLRKWR
310	320	330	340	350
KSLRPRMLDE	LSLPKFSLSQ	DYNLNDILPE	LGIKEVFSTQ	ADLSGITGAK
360	370	380	390	400
NIRVSQMIHQ	AALDVTETHT	EADVITIARY	NFQSAKIKAK	IVKVDREFLY
410	420	422
LILDPMFKSI	SVMGKVINPL	TN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3500 Collapse assay details

Data source: Panorama

Official Gene Symbol: Serpina3a
Peptide Sequence: IQHVEDSLQPQSLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 284
Peptide End: 297

CPTAC ID: non-CPTAC-3500
Peptide Molecular Mass: 1,648.8533
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b9 (2+)	6	2.8	3.5	6.7	3.8	3.6	9	4.7	5	15	15	15
b8 (2+)	15.6	7.6	5.6	15.5	10.5	6.7	22	13	8.7	15	15	15
b7 (1+)	10.1	2.1	6.4	11.5	7.2	8.4	15.3	7.5	10.6	15	15	15
y5 (2+)	9.3	3.3	3.5	12.5	3.9	4.4	15.6	5.1	5.6	15	15	15
y5 (1+)	28.2	3	3.5	39.8	5.4	3.9	48.8	6.2	5.2	15	15	15
sum	15.9	2	1.5	20.7	3.8	2.2	26.1	4.3	2.7	15	15	15

Serpina3a

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3500 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Serpina3a

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3500 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments