A1bg, CPTAC-3518 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
A1bg	N/A

Sequence Length (AA)	Molecular Weight (Da)
512	56554

Protein Name
Alpha-1B-glycoprotein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSLLATVLLL	WGFTLGPGNT	LMLDSGSEPK	LWAEPQSLLE	PWANLTLVCA
60	70	80	90	100
VDLPTKVFEL	IQNGWFLSQV	RLETQVLSYR	FSLGAITSNN	SGIYRCRCGV
110	120	130	140	150
EPPVDIHLPA	LNKWTMLSNA	VEVTGKEPLP	RPLAHADPVD	WITPGGLPVY
160	170	180	190	200
VMCQVAMRGV	TYLLRQEGVD	GVQKPDVQHK	GTAGFLIYKP	GNYSCSYLTH
210	220	230	240	250
AAGEPSEPSD	IVTIKMYASQ	APPTLCLMGN	YLMIYPQKTY	ETLACKAPRN
260	270	280	290	300
AAEFQLRQGG	KVLKIHGFSP	TRDAILYYVN	LKELDNPGPF	TCRYRMHKYM
310	320	330	340	350
HVWSEDSKPV	ELMWSDETLQ	APVLTAEPSS	RDLEPGSTVQ	LRCTAPVSGL
360	370	380	390	400
RFGLQRQGKP	ELVVVQMLNS	SGTEAVFELH	NISTIDSGNY	SCIYMEQAPP
410	420	430	440	450
FSGSSSSEPV	ELRVNGPPPK	PRLEALWKST	VHLGQEAIFR	CHGHVPRVSM
460	470	480	490	500
ELVREGFKTP	FAVASTRSTS	AYLKLLFVGP	QHAGNYSCRY	TALPPFTFES
510	512
GISDPVEVIV	EG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-1064 Collapse assay details

Data source: Panorama

Official Gene Symbol: A1BG
Peptide Sequence: LETPDFQLFK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 53
Peptide End: 62

CPTAC ID: non-CPTAC-1064
Peptide Molecular Mass: 1,236.6390
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	7.1	4.2	5.4	9.8	5.6	6.3	12.1	7	8.3	15	15	15
y8 (2+)	11.1	4.4	3.5	12.2	9.3	4.4	16.5	10.3	5.6	15	15	15
y7 (1+)	6.4	5	4.1	10.8	5.5	5.3	12.6	7.4	6.7	15	15	15
sum	5.9	2.8	2.7	9.4	5.2	3.8	11.1	5.9	4.7	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3518 Collapse assay details

Data source: Panorama

Official Gene Symbol: A1bg
Peptide Sequence: TPFAVASTR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 459
Peptide End: 467

CPTAC ID: non-CPTAC-3518
Peptide Molecular Mass: 948.5029
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.