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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Igh-3 N/A
Sequence Length (AA) Molecular Weight (Da)
404 44259
Protein Name
Ig gamma-2B chain C region
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
KTTPPSVYPL APGCGDTTGS SVTLGCLVKG YFPESVTVTW NSGSLSSSVH
60 70 80 90 100
TFPALLQSGL YTMSSSVTVP SSTWPSQTVT CSVAHPASST TVDKKLEPSG
110 120 130 140 150
PISTINPCPP CKECHKCPAP NLEGGPSVFI FPPNIKDVLM ISLTPKVTCV
160 170 180 190 200
VVDVSEDDPD VQISWFVNNV EVHTAQTQTH REDYNSTIRV VSTLPIQHQD
210 220 230 240 250
WMSGKEFKCK VNNKDLPSPI ERTISKIKGL VRAPQVYILP PPAEQLSRKD
260 270 280 290 300
VSLTCLVVGF NPGDISVEWT SNGHTEENYK DTAPVLDSDG SYFIYSKLNM
310 320 330 340 350
KTSKWEKTDS FSCNVRHEGL KNYYLKKTIS RSPGLDLDDI CAEAKDGELD
360 370 380 390 400
GLWTTITIFI SLFLLSVCYS ASVTLFKVKW IFSSVVELKQ KISPDYRNMI
404
GQGA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3524 Collapse assay details

Data source: Panorama

Official Gene Symbol
Igh-3
Peptide Sequence
DLPSPIER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
215
Peptide End
222
CPTAC ID
non-CPTAC-3524
Peptide Molecular Mass
925.4869
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 5 4.5 4.5 15.9 6.2 5.9 16.7 7.7 7.4 15 15 15
y4 (2+) 4.7 3.2 5.1 13.7 5.1 5.9 14.5 6 7.8 15 15 15
y6 (2+) 3.9 2.7 3 12.5 3.4 7.2 13.1 4.3 7.8 15 15 15
y4 (1+) 4.6 5.2 4.1 16.3 6.4 5 16.9 8.2 6.5 15 15 15
y6 (1+) 5.6 6.2 4.1 16 6.1 9.1 17 8.7 10 15 15 15
sum 2.6 1.7 1.9 13.2 3.2 4.8 13.5 3.6 5.2 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3525 Collapse assay details

Data source: Panorama

Official Gene Symbol
Igh-3
Peptide Sequence
DTAPVLDSDGSYFIYSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
281
Peptide End
297
CPTAC ID
non-CPTAC-3525
Peptide Molecular Mass
1,876.8731
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b8 (1+) 7.6 4.1 5.9 17 14.6 17.6 18.6 15.2 18.6 15 15 15
y14 (2+) 5.6 3 3 14.6 3.5 5.8 15.6 4.6 6.5 15 15 15
y15 (2+) 8.2 3 2.9 14.5 4 7.1 16.7 5 7.7 15 15 15
y2 (1+) 6.6 2.8 3.4 16.3 4.8 9.6 17.6 5.6 10.2 15 15 15
y3 (1+) 5.6 4.9 2.9 16.2 5.1 5.7 17.1 7.1 6.4 15 15 15
sum 3.9 2.1 2.4 13.3 3.6 6.8 13.9 4.2 7.2 15 15 15

Additional Resources and Comments