Igh-3, CPTAC-3524 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Igh-3	N/A

Sequence Length (AA)	Molecular Weight (Da)
404	44259

Protein Name
Ig gamma-2B chain C region

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
KTTPPSVYPL	APGCGDTTGS	SVTLGCLVKG	YFPESVTVTW	NSGSLSSSVH
60	70	80	90	100
TFPALLQSGL	YTMSSSVTVP	SSTWPSQTVT	CSVAHPASST	TVDKKLEPSG
110	120	130	140	150
PISTINPCPP	CKECHKCPAP	NLEGGPSVFI	FPPNIKDVLM	ISLTPKVTCV
160	170	180	190	200
VVDVSEDDPD	VQISWFVNNV	EVHTAQTQTH	REDYNSTIRV	VSTLPIQHQD
210	220	230	240	250
WMSGKEFKCK	VNNKDLPSPI	ERTISKIKGL	VRAPQVYILP	PPAEQLSRKD
260	270	280	290	300
VSLTCLVVGF	NPGDISVEWT	SNGHTEENYK	DTAPVLDSDG	SYFIYSKLNM
310	320	330	340	350
KTSKWEKTDS	FSCNVRHEGL	KNYYLKKTIS	RSPGLDLDDI	CAEAKDGELD
360	370	380	390	400
GLWTTITIFI	SLFLLSVCYS	ASVTLFKVKW	IFSSVVELKQ	KISPDYRNMI
404
GQGA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-3524 Collapse assay details

Data source: Panorama

Official Gene Symbol: Igh-3
Peptide Sequence: DLPSPIER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 215
Peptide End: 222

CPTAC ID: non-CPTAC-3524
Peptide Molecular Mass: 925.4869
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	5	4.5	4.5	15.9	6.2	5.9	16.7	7.7	7.4	15	15	15
y4 (2+)	4.7	3.2	5.1	13.7	5.1	5.9	14.5	6	7.8	15	15	15
y6 (2+)	3.9	2.7	3	12.5	3.4	7.2	13.1	4.3	7.8	15	15	15
y4 (1+)	4.6	5.2	4.1	16.3	6.4	5	16.9	8.2	6.5	15	15	15
y6 (1+)	5.6	6.2	4.1	16	6.1	9.1	17	8.7	10	15	15	15
sum	2.6	1.7	1.9	13.2	3.2	4.8	13.5	3.6	5.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3525 Collapse assay details

Data source: Panorama

Official Gene Symbol: Igh-3
Peptide Sequence: DTAPVLDSDGSYFIYSK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 281
Peptide End: 297

CPTAC ID: non-CPTAC-3525
Peptide Molecular Mass: 1,876.8731
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers