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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Serpina1d Dom4, Spi1-4
Sequence Length (AA) Molecular Weight (Da)
413 45998
Protein Name
Alpha-1-antitrypsin 1-4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTPSISWSLL LLAGLCCLVP SFLAEDVQET DTSQKDQSPA SHEIATNLGD
60 70 80 90 100
FALRLYRELV HQSNTSNIFF SPVSIATAFA MLSLGSKGDT HTQILEGLQF
110 120 130 140 150
NLTQTSEADI HKSFQHLLQT LNRPDSELQL STGNGLFVNN DLKLVEKFLE
160 170 180 190 200
EAKNHYQAEV FSVNFAESEE AKKVINDFVE KGTQGKIVEA VKKLDQDTVF
210 220 230 240 250
ALANYILFKG KWKQPFDPEN TEEAEFHVDE STTVKVPMMT LSGMLDVHHC
260 270 280 290 300
SMLSSWVLLM DYAGNTTAVF LLPDDGKMQH LEQTLNKELI SQFLLNRRRS
310 320 330 340 350
DAQIHIPRLS ISGNYNLKTL MSPLGITRIF NNGADLSGIT EENAPLKLSK
360 370 380 390 400
AVHKAVLTID ETGTEAAAAT VLQVATYSMP PIVRFDHPFL FIIFEEHTQS
410 413
PIFVGKVVDP THK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3529 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serpina1d
Peptide Sequence
ELISQFLLNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
288
Peptide End
297
CPTAC ID
non-CPTAC-3529
Peptide Molecular Mass
1,231.6925
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 4.4 3.9 3.2 9.7 4.9 8.4 10.7 6.3 9 15 15 15
y3 (1+) 3.6 4.3 4.6 10.3 5.4 6.8 10.9 6.9 8.2 15 15 15
y4 (1+) 4.9 5.8 5.1 14.4 5.7 6.2 15.2 8.1 8 15 15 15
y5 (1+) 4.3 2.9 2.9 12.5 4.3 6.1 13.2 5.2 6.8 15 15 15
y7 (1+) 5.8 4.4 3.3 16.9 6.4 4 17.9 7.8 5.2 15 15 15
sum 3.1 1.1 1.3 12.8 3.1 4.9 13.2 3.3 5.1 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3530 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serpina1d
Peptide Sequence
LSISGNYNLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
309
Peptide End
318
CPTAC ID
non-CPTAC-3530
Peptide Molecular Mass
1,107.5924
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 2.8 4.3 5.2 16.2 6.6 8.3 16.4 7.9 9.8 15 15 15
y6 (1+) 8.3 5.6 4.1 17.9 12 10.9 19.7 13.2 11.6 15 15 15
y7 (1+) 3.1 4.5 6 13.1 5.3 7.1 13.5 7 9.3 15 15 15
y8 (1+) 3.5 7.3 6 11.9 8.9 8.2 12.4 11.5 10.2 15 15 15
y9 (1+) 4.4 5.4 6.9 15.4 5.8 6.9 16 7.9 9.8 15 15 15
sum 1.6 1.7 2.6 14 4.8 5.5 14.1 5.1 6.1 15 15 15


Additional Resources and Comments