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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Itih4 N/A
Sequence Length (AA) Molecular Weight (Da)
942 104659
Protein Name
Inter alpha-trypsin inhibitor, heavy chain 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKSPAPAHMW NLVLFLPSLL AVLPTTTAEK NGIDIYSLTV DSRVSSRFAH
60 70 80 90 100
TVVTSRVVNR ADAVQEATFQ VELPRKAFIT NFSMIIDGVT YPGVVKEKAE
110 120 130 140 150
AQKQYSAAVG RGESAGIVKT TGRQTEKFEV SVNVAPGSKI TFELIYQELL
160 170 180 190 200
QRRLGMYELL LKVRPQQLVK HLQMDIYIFE PQGISILETE STFMTPELAN
210 220 230 240 250
ALTTSQNKTK AHIRFKPTLS QQQKSQSEQD TVLNGDFIVR YDVNRSDSGG
260 270 280 290 300
SIQIEEGYFV HHFAPENLPT MSKNVIFVID KSGSMSGKKI QQTREALVKI
310 320 330 340 350
LKDLSPQDQF NLIEFSGEAN QWKQSLVQAT EENLNKAVNY ASRIRAHGGT
360 370 380 390 400
NINNAVLLAV ELLDRSNQAE LLPSKSVSLI ILLTDGDPTV GETNPTIIQN
410 420 430 440 450
NVREAINGQY SLFCLGFGFD VNYPFLEKMA LDNGGLARRI YEDSDSALQL
460 470 480 490 500
QDFYHEVANP LLSSVAFEYP SDAVEEVTRY KFQHHFKGSE MVVAGKLQDQ
510 520 530 540 550
GPDVLLAKVS GQMHMQNITF QTEASVAQQE KEFKSPKYIF HNFMERLWAL
560 570 580 590 600
LTIQQQLEQR ISASGAELEA LEAQVLNLSL KYNFVTPLTH MVVTKPEGQE
610 620 630 640 650
QFQVAEKPVE VGDGMQRLPL AAQAHPFRPP VRGSKLMTVL KGSRSQIPRL
660 670 680 690 700
GDAVRASRQY IPPGFPGPPG PPGFPAPPGP PGFPAPPGPP LASGSDFSLQ
710 720 730 740 750
PSYERMLSLP SVAAQYPADP HLVVTEKSKE STIPEESPNP DHPQVPTITL
760 770 780 790 800
PLPGSSVDQL CVDILHSEKP MKLFVDPSQG LEVTGKYENT GFSWLEVTIQ
810 820 830 840 850
KPHLQVHATP ERLVVTRGRK NTEYKWKKTL FSVLPGLKMT MNMMGLLQLS
860 870 880 890 900
GPDKVTIGLL SLDDPQRGLM LLLNDTQHFS NNVKGELGQF YRDIVWEPPV
910 920 930 940 942
EPDNTKRTVK VQGVDYLATR ELKLSYQEGF PGAEISCWTV EI

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3554 Collapse assay details

Data source: Panorama

Official Gene Symbol
Itih4
Peptide Sequence
TLFSVLPGLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
829
Peptide End
838
CPTAC ID
non-CPTAC-3554
Peptide Molecular Mass
1,073.6485
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 3.1 1.9 2.2 14.1 5.1 5.7 14.4 5.4 6.1 15 15 15
b2 (1+) 2 1.9 1.4 13.1 4.4 6.8 13.3 4.8 6.9 15 15 15
y8 (2+) 2 1.8 2.2 15.4 3.5 6.9 15.5 3.9 7.2 15 15 15
y4 (1+) 1.6 2.4 1.7 13.2 3.8 5.6 13.3 4.5 5.9 15 15 15
y7 (1+) 2.9 2.9 3.5 14.8 4.7 6.7 15.1 5.5 7.6 15 15 15
sum 1.2 0.6 0.7 13.7 3.8 5.7 13.8 3.8 5.7 15 15 15


Additional Resources and Comments