Adamdec1, CPTAC-3557 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Adamdec1	N/A

Sequence Length (AA)	Molecular Weight (Da)
467	52956

Protein Name
ADAM DEC1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLPGTSRLPT	EASMSWVLLS	VLWLIIQIQV	IDATLTPELK	PHEIVRPKKL
60	70	80	90	100
PISQKRGLEN	NQTERYGKEE	KYAPEVQYQI	ILNGEEIVFH	LKRTKHLLGP
110	120	130	140	150
DYTETSYSPR	GEESTRHSQD	VKPCYYEGHI	QNARGSLARI	STCDGLRGYF
160	170	180	190	200
THRDQRYQIK	PLQSTDEGEH	AVLPYSWKGQ	DTVHDKDAEK	QVVRKRSHLR
210	220	230	240	250
TSRSLKNPNE	DLLQGQKYIG	LFLVLDNAYY	KLYNGNVTQM	RTFLFKVLNL
260	270	280	290	300
LNMIYKTINI	QVSLVGMEIW	SDQDKIKVEP	NLGATFTHFM	RWHYSNLGKR
310	320	330	340	350
IHNHAQLLSG	ASFRHGRVGM	AAGNSFCTTS	SVSVIEAKKK	NNVALVALMS
360	370	380	390	400
HELGHALGMK	DVPYYTKCPS	GSCVMNQYLS	SKFPKDFSTV	SRSHFQGFLS
410	420	430	440	450
SRNARCLLLA	PDPKNIIKPT	CGNQVLDVGE	ECDCGSPEEC	TNLCCEPLTC
460	467
RLKSQPDCSE	ASNHITE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3557 Collapse assay details

Data source: Panorama

Official Gene Symbol: Adamdec1
Peptide Sequence: IHNHAQLLSGASFR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 301
Peptide End: 314

CPTAC ID: non-CPTAC-3557
Peptide Molecular Mass: 1,549.8114
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b8 (2+)	16.8	11.3	8	18.7	13.9	10.2	25.1	17.9	13	15	15	15
b7 (2+)	22.8	5.6	6.1	20.2	6.1	7.4	30.5	8.3	9.6	15	15	15
b6 (2+)	9.7	7.7	5.3	12.4	6.8	6.5	15.7	10.3	8.4	15	15	15
y6 (1+)	10.5	4.3	4.8	11.5	8.6	6.6	15.6	9.6	8.2	15	15	15
y7 (1+)	19.1	6.7	4.2	19.5	9.3	3.7	27.3	11.5	5.6	15	15	15
sum	8.3	3.9	2.9	9.1	6.6	4.3	12.3	7.7	5.2	15	15	15

Adamdec1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3557 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Adamdec1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3557 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments