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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Hgfac N/A
Sequence Length (AA) Molecular Weight (Da)
653 70568
Protein Name
Hepatocyte growth factor activator
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGRQAWISSL CPLPRPCPFL LLLLLLVVPR GAQPQAGRNH TEPPGPNVTA
60 70 80 90 100
TPVTPTIPVI SGNVSTSTES APAAETEGPQ SERYPPPSSS SPPGGQVLTE
110 120 130 140 150
SGQPCRFPFR YGGRMLHSCT SEGSAYRKWC ATTHNYDRDR AWGYCAEVTL
160 170 180 190 200
PVEGPAILDP CASGPCLNGG TCSSTHDHGS YHCSCPLAFT GKDCGTEKCF
210 220 230 240 250
DETRYEYFEV GDHWARVSEG HVEQCGCMEG QARCEDTHHT ACLSSPCLNG
260 270 280 290 300
GTCHLIVGTG TSVCTCPLGY AGRFCNIVPT EHCFLGNGTE YRGVASTAAS
310 320 330 340 350
GLSCLAWNSD LLYQELHVDS VAAAVLLGLG PHAYCRNPDK DERPWCYVVK
360 370 380 390 400
DNALSWEYCR LTACESLARV HSQTPEILAA LPESAPAVRP TCGKRHKKRT
410 420 430 440 450
FLRPRIIGGS SSLPGSHPWL AAIYIGNSFC AGSLVHTCWV VSAAHCFANS
460 470 480 490 500
PPRDSITVVL GQHFFNRTTD VTQTFGIEKY VPYTLYSVFN PNNHDLVLIR
510 520 530 540 550
LKKKGERCAV RSQFVQPICL PEAGSSFPTG HKCQIAGWGH MDENVSSYSN
560 570 580 590 600
SLLEALVPLV ADHKCSSPEV YGADISPNML CAGYFDCKSD ACQGDSGGPL
610 620 630 640 650
VCEKNGVAYL YGIISWGDGC GRLNKPGVYT RVANYVDWIN DRIRPPKRPV
653
ATS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3581 Collapse assay details

Data source: Panorama

Official Gene Symbol
Hgfac
Peptide Sequence
TTDVTQTFGIEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
468
Peptide End
479
CPTAC ID
non-CPTAC-3581
Peptide Molecular Mass
1,338.6667
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 8.4 8.2 6.1 10.4 6.7 8.5 13.4 10.6 10.5 15 15 15
y10 (1+) 9.6 7.4 6.7 18 9.9 8.7 20.4 12.4 11 15 15 15
b4 (1+) 27 5.8 7.9 29.7 6.1 9.1 40.1 8.4 12.1 15 15 15
b3 (1+) 12.2 6.8 3.6 12.7 7 4.1 17.6 9.8 5.5 15 15 15
b2 (1+) 12.3 5.3 3.9 14.4 7.7 9.6 18.9 9.3 10.4 15 15 15
sum 3.9 2.7 2.8 7.8 5.2 5.9 8.7 5.9 6.5 15 15 15


Additional Resources and Comments