Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Oaf D9Ucla1
Sequence Length (AA) Molecular Weight (Da)
282 31524
Protein Name
Out at first protein homolog
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRPPGCRDVP SARPALPLLL LLLSPLLLGA LHGVGAGSGA PAELRVRVRL
60 70 80 90 100
PDSQVIEESL QADSDADSIS LDLRKPDGTL ISFIADFKKD VKIFRALILG
110 120 130 140 150
ELEKGQSQFQ ALCFVTRLHH NDIIPSEAMA KLRQKNPRAV RQAEEVRGLE
160 170 180 190 200
QLHMDIAVNF SQGGLLSPHL HNVCAEATDA IYTRQEDVQF WTERGVDSSV
210 220 230 240 250
FEALPKALEQ AELPRCGRVG DRGKPCTCHY SLSLAWYPCM LKYCHSRDRP
260 270 280 282
APYKCGIRSC RKSYTFDFYV PQKQLCLWDE DP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-3598 Collapse assay details

Data source: Panorama

Official Gene Symbol
Oaf
Peptide Sequence
GVDSSVFEALPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
195
Peptide End
206
CPTAC ID
non-CPTAC-3598
Peptide Molecular Mass
1,247.6398
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 5.4 3.2 1.5 6.5 7.2 4.7 8.5 7.9 4.9 15 15 15
y6 (1+) 8.3 2 2.8 9.6 7.7 4.5 12.7 8 5.3 15 15 15
y7 (1+) 19.4 4.2 2.4 22.9 8.4 4.5 30 9.4 5.1 15 15 15
y8 (1+) 9.3 2.4 2.9 11.6 7.7 4.9 14.9 8.1 5.7 15 15 15
y10 (1+) 3.9 1.4 1.8 7.3 6.7 5.4 8.3 6.8 5.7 15 15 15
sum 3.9 0.7 1.1 5.8 6.9 4.6 7 6.9 4.7 15 15 15


Additional Resources and Comments