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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Ptprf

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Ptprf Lar
Sequence Length (AA) Molecular Weight (Da)
1898 211489
Protein Name
Receptor-type tyrosine-protein phosphatase F
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAPEPAPGRR MVPLVPALVM LGLMAGAHGD SKPVFVKVPE DQTGLSGGVA
60 70 80 90 100
SFVCQATGEP KPRITWMKKG KKVSSQRFEV IEFDDGAGSV LRIQPLRVQR
110 120 130 140 150
DEAIYECTAT NSLGEINTSA KLSVLEEDQL PSGFPTIDMG PQLKVVEKGR
160 170 180 190 200
TATMLCAAGG NPDPEISWFK DFLPVDPAAS NGRIKQLRSG ALQIESSEES
210 220 230 240 250
DQGKYECVAT NSAGTRYSAP ANLYVRVRRV APRFSIPPSS QEVMPGGSVN
260 270 280 290 300
LTCVAVGAPM PYVKWMMGAE ELTKEDEMPV GRNVLELSNV MRSANYTCVA
310 320 330 340 350
ISSLGMIEAT AQVTVKALPK PPIDLVVTET TATSVTLTWD SGNTEPVSFY
360 370 380 390 400
GIQYRAAGTD GPFQEVDGVA STRYSIGGLS PFSEYAFRVL AVNSIGRGPP
410 420 430 440 450
SEAVRARTGE QAPSSPPRRV QARMLSASTM LVQWEPPEEP NGLVRGYRVY
460 470 480 490 500
YTPDSRRPLS AWHKHNTDAG LLTTVGSLLP GITYSLRVLA FTAVGDGPPS
510 520 530 540 550
PTIQVKTQQG VPAQPADFQA NAESDTRIQL SWLLPPQERI VKYELVYWAA
560 570 580 590 600
EDEGQQHKVT FDPTSSYTLE DLKPDTLYHF QLAARSDLGV GVFTPTVEAR
610 620 630 640 650
TAQSTPSAPP QKVTCVSTGS TTVRVSWVPP PADSRNGIIT QYSVAYEAVD
660 670 680 690 700
GEDRKRHVVD GISREHSSWD LLGLEKWTEY RVWVRAHTDV GPGPESSPVL
710 720 730 740 750
VRTDEDVPSG PPRKVEVEPL NSTAVHVSWK LPVPNKQHGQ IRGYQVTYVR
760 770 780 790 800
LENGEPRGQP IIQDVMLAEA QETTISGLTP ETTYSITVAA YTTKGDGARS
810 820 830 840 850
KPKVVTTTGA VPGRPTMMVS TTAMHTALLQ WHPPKELPGE LLGYRLQYRR
860 870 880 890 900
ADEARPNTID FGKDDQHFTV TGLHKGATYV FRLAAKNRAG PGEEFEKEIT
910 920 930 940 950
TPEDVPSGFP QNLRVTGLTT STTELTWDPP VLAERNGHIT NYTVVYRDIN
960 970 980 990 1000
SQLELQNVTN DTHLTLLGLK PDTTYDIKVR AHTSKGAGPL SPSIQSRTMP
1010 1020 1030 1040 1050
VEQVFAKNFR VAAAMKTSVL LSWEVPDSYK SAVPFKILYN GQSVEVDGHS
1060 1070 1080 1090 1100
MRKLIADLQP NTEYSFVLMN RGSSAGGLQH LVSIRTAPDL LPQKPLPASA
1110 1120 1130 1140 1150
FIEDGRFSLS MPQVQDPSLV RWFYIVVVPI DRVGGNLLAP RWNTPEELEL
1160 1170 1180 1190 1200
DELLEAIEQG EEKQRRRRRQ AERLKPYVAA QVDVLPDTFT LGDKKSYRGF
1210 1220 1230 1240 1250
YNRPLSPDLS YQCFVLASLK EPMDQKRYAS SPYSDEIVVQ VTPAQQQEEP
1260 1270 1280 1290 1300
EMLWVTGPVL AVILIILIVI AILLFKRKRT HSPSSKDEQS IGLKDSLLAH
1310 1320 1330 1340 1350
SSDPVEMRRL NYQTPGMRDH PPIPITDLAD NIERLKANDG LKFSQEYESI
1360 1370 1380 1390 1400
DPGQQFTWEN SNSEVNKPKN RYANVIAYDH SRVLLTSIDG VPGSDYINAN
1410 1420 1430 1440 1450
YIDGYRKQNA YIATQGPLPE TMGDFWRMVW EQRTATVVMM TRLEEKSRVK
1460 1470 1480 1490 1500
CDQYWPVRGT ETYGLIQVTL VDTVELATYT MRTFALHKSG SSEKRELRQF
1510 1520 1530 1540 1550
QFMAWPDHGV PEYPTPILAF LRRVKACNPL DAGPMVVHCS AGVGRTGCFI
1560 1570 1580 1590 1600
VIDAMLERMK HEKTVDIYGH VTCMRSQRNY MVQTEDQYVF IHEALLEAAM
1610 1620 1630 1640 1650
CGHTEVLARN LYAHIQKLGQ VPPGESVTAM ELEFKLLANS KAHTSRFVSA
1660 1670 1680 1690 1700
NLPCNKFKNR LVNIMPYELT RVCLQPIRGV EGSDYINASF LDGYRQQKAY
1710 1720 1730 1740 1750
IATQGPLAES TEDFWRMLWE HNSTIIVMLT KLREMGREKC HQYWPAERSA
1760 1770 1780 1790 1800
RYQYFVVDPM AEYNMPQYIL REFKVTDARD GQSRTIRQFQ FTDWPEQGVP
1810 1820 1830 1840 1850
KTGEGFIDFI GQVHKTKEQF GQDGPITVHC SAGVGRTGVF ITLSIVLERM
1860 1870 1880 1890 1898
RYEGVVDMFQ TVKTLRTQRP AMVQTEDQYQ LCYRAALEYL GSFDHYAT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3600 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ptprf
Peptide Sequence
GSSAGGLQHLVSIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1072
Peptide End
1085
CPTAC ID
non-CPTAC-3600
Peptide Molecular Mass
1,380.7474
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (2+) 20.2 12.2 6.1 25.7 12.9 8 32.7 17.8 10.1 15 15 15
y10 (2+) 8.8 9.2 4.6 12.3 10.2 7.3 15.1 13.7 8.6 15 15 15
y11 (2+) 15.5 12.8 9 16.5 9.4 10.3 22.6 15.9 13.7 15 15 15
y12 (2+) 15.6 7.6 5.4 16.6 9 8.6 22.8 11.8 10.2 15 15 15
y4 (1+) 27.9 9 9.9 28.1 10.7 11.7 39.6 14 15.3 15 15 15
sum 8.7 4.7 2.3 8.8 6.1 5.2 12.4 7.7 5.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments