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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Papln

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Papln N/A
Sequence Length (AA) Molecular Weight (Da)
1280 138904
Protein Name
Papilin
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQLFPLLFSL LLTSTPGSWA RNVRRQSDTW GTWGEWSPCS RTCGGGISFR
60 70 80 90 100
ERPCYSQRRD GGTSCVGPAR SHRTCHTESC PDGVRDFRAE QCAEFDGTDF
110 120 130 140 150
QGRRYRWLPY YAAPNKCELN CIPKGQNFYY KHKDAVVDGT PCEPGQRDIC
160 170 180 190 200
VDGVCRVVGC DHKLDSIKQE DKCLQCGGDG SSCYPVTGTF DGNDLSRGYN
210 220 230 240 250
QIFIIPAGAT SIRIEEAAAS RNFLAVKSIR GEYYLNGHWT IEAAQALPVA
260 270 280 290 300
STVLQYERGV EGDLAPERLQ ARGPTSEPLV IELLSQESNP GVHYEYYLPA
310 320 330 340 350
NDPGRGFSWS HGSWGDCSAE CGGGHQSRLV FCTIDNEAYP DHMCQHQPRP
360 370 380 390 400
THRRSCNTQP CPKTKRWKVG PWTPCSVSCG GGVQSRSVYC ISSDGTGGQE
410 420 430 440 450
AAEETQCAGL VGKPPTTQAC NLQHCAVWSV EPWGECSVTC GTGIRKRSVT
460 470 480 490 500
CRGDEGSPVH AAACLLKDQP TLTEPCVQEA CPVFRGQAWH VGSWSLCSKS
510 520 530 540 550
CGSGIRRRQV VCTIGPPGRC VDLQSSKPAE MEACNRQPCH LPQEVPSIQD
560 570 580 590 600
PRTRSSDPRM LSGPRVSPVS DGREQQWAPL ERPRAQSNPR EGQDPNLSSA
610 620 630 640 650
GRAPTLQRPP HQPPLRPSSG PRDCRHSPHG CCPDGHTPSL GPQWQGCPLA
660 670 680 690 700
GASCLQSRYG CCPDGVSAAE GPQQAGCTRS HGSDNTGNRP GSRAVASKNP
710 720 730 740 750
KIHQPQAHEG EPSECRSSRF GCCYDNVASA AGPLGEGCVG QPSYAYPVRC
760 770 780 790 800
LLPSAQGSCG DWAARWYFVA SVGRCNRFWY GGCHGNANNF ASEQECMNTC
810 820 830 840 850
RGQHGPRRPE AGAAGHRAHV DGGQRGPGGQ QEPDWHRAGA TIPRLPSPSG
860 870 880 890 900
SPWRREQEPA PGEPPHIPAY GNRPGGQEIR PRVPGLDREA RPAVPPTHSP
910 920 930 940 950
SYRIRLAGSE PSLVQAAPGQ AVQLFCPGNI PSEFQAGWQK EGRPISSNRY
960 970 980 990 1000
QLQADGSLII SRLRPEDAGI YSCGSHRPGH EPQEIQLRVT GGDMAVFPEG
1010 1020 1030 1040 1050
QPRHFPEPRN PDLGHGPPHR GTGAEAGGHR VLSPSHPRPA TRLRLDRTQP
1060 1070 1080 1090 1100
GVVDASPGQR IRLTCRAEGF PVPTIEWQRD GQLVSSPRHQ VQPDGSLVIS
1110 1120 1130 1140 1150
RVDVEDGGYY SCVAFNGQDR DQRWVQLRVL RELTITGLPP AVTVAEGDTA
1160 1170 1180 1190 1200
RLLCVVAGES VNIRWSRNGL PIQADGHRVH QSPDGTLLIH NLRPRDEGSY
1210 1220 1230 1240 1250
TCSAFRGSQA VSRSTEVKVA LPAPAAQSRD LGKDCIDQPE LANCALILQA
1260 1270 1280
QLCGNEYYSS FCCASCSRFQ PNAQPVWQQG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3604 Collapse assay details

Data source: Panorama

Official Gene Symbol
Papln
Peptide Sequence
VALPAPAAQSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1219
Peptide End
1229
CPTAC ID
non-CPTAC-3604
Peptide Molecular Mass
1,079.6087
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (2+) 26.9 4.7 3.7 33.4 7.6 6.9 42.9 8.9 7.8 15 15 15
y6 (1+) 12.8 6.6 3.6 13.1 6.9 5.1 18.3 9.5 6.2 15 15 15
y8 (1+) 17.4 5.6 4.7 20.5 6.8 7.3 26.9 8.8 8.7 15 15 15
y6 (2+) 21.5 7.3 2.9 21.3 8.7 4.7 30.3 11.4 5.5 15 15 15
y8 (2+) 13.4 3.5 5.5 13.8 7.7 7.1 19.2 8.5 9 15 15 15
sum 8.5 2.5 2.7 8.8 5.6 5.6 12.2 6.1 6.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments