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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Vwf

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Vwf N/A
Sequence Length (AA) Molecular Weight (Da)
2813 309269
Protein Name
von Willebrand factor
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MNPFRYEICL LVLALTWPGT LCTEKPRDRP STARCSLFGD DFINTFDETM
60 70 80 90 100
YSFAGGCSYL LAGDCQKRSF SILGNFQDGK RMSLSVYLGE FFDIHLFANG
110 120 130 140 150
TVMQGDQSIS MPYASQGLYL ELEAGYYKLS SETFGFAARI DGNGNFQVLM
160 170 180 190 200
SDRHFNKTCG LCGDFNIFAE DDFRTQEGTL TSDPYDFANS WALSSEEQRC
210 220 230 240 250
KRASPPSRNC ESSSGDMHQA MWEQCQLLKT ASVFARCHPL VDPESFVALC
260 270 280 290 300
EKILCTCATG PECACPVLLE YARTCAQEGM VLYGWTDHSA CRPACPAGME
310 320 330 340 350
YKECVSPCPR TCQSLSINEV CQQQCVDGCS CPEGELLDED RCVQSSDCPC
360 370 380 390 400
VHAGKRYPPG TSLSQDCNTC ICRNSLWICS NEECPGECLV TGQSHFKSFD
410 420 430 440 450
NRYFTFSGIC QYLLARDCED HTFSIVIETM QCADDPDAVC TRSVSVRLSA
460 470 480 490 500
LHNSLVKLKH GGAVGIDGQD VQLPFLQGDL RIQHTVMASV RLSYAEDLQM
510 520 530 540 550
DWDGRGRLLV KLSPVYSGKT CGLCGNYNGN KGDDFLTPAG LVEPLVVDFG
560 570 580 590 600
NAWKLQGDCS DLRRQHSDPC SLNPRLTRFA EEACALLTSS KFEACHHAVS
610 620 630 640 650
PLPYLQNCRY DVCSCSDSRD CLCNAVANYA AECARKGVHI GWREPGFCAL
660 670 680 690 700
GCPQGQVYLQ CGNSCNLTCR SLSLPDEECS EVCLEGCYCP PGLYQDERGD
710 720 730 740 750
CVPKAQCPCY YDGELFQPAD IFSDHHTMCY CEDGFMHCTT SGTLGSLLPD
760 770 780 790 800
TVLSSPLSHR SKRSLSCRPP MVKLVCPADN PRAQGLECAK TCQNYDLERM
810 820 830 840 850
SLGCVSGCLC PPGMVRHENK CVALERCPCF HQGAEYAPGD TVKIGCNTCV
860 870 880 890 900
CRERKWNCTN HVCDATRSAI GMAHYLTFDG LKYLFPGECQ YVLVYDYCGS
910 920 930 940 950
NPGTFQILVG NEGCSYPSVK CRKRVTILVD GGELELFDGE VNVKRPLRDE
960 970 980 990 1000
SHFEVVESGR YVILLLGQAL SVVWDHHLSI SVVLKHTYQE QVCGLCGNFD
1010 1020 1030 1040 1050
GIQNNDSTTS SLQVEEDPVN FGNSWKVSSQ CADTRKLSLD VSPATCHNNI
1060 1070 1080 1090 1100
MKQTMVDSAC RILTSDVFQG CNRLVDPEPY LDICIYDTCS CESIGDCACF
1110 1120 1130 1140 1150
CDTIAAYAHV CAQHGQVVAW RTPTLCPQSC EEKNVRENGY ECEWRYNSCA
1160 1170 1180 1190 1200
PACPVTCQHP EPLACPVQCV EGCHAHCPPG RILDELLQTC VDPQDCPVCE
1210 1220 1230 1240 1250
VAGRRLAPGK KITLSPDDPA HCQNCHCDGV NLTCEACQEP GGLVAPPTDA
1260 1270 1280 1290 1300
PVSSTTPYVE DTPEPPLHNF YCSKLLDLVF LLDGSSMLSE AEFEVLKAFV
1310 1320 1330 1340 1350
VGMMERLHIS QKRIRVAVVE YHDGSRAYLE LKARKRPSEL RRITSQIKYT
1360 1370 1380 1390 1400
GSQVASTSEV LKYTLFQIFG KIDRPEASHI TLLLTASQEP PRMARNLVRY
1410 1420 1430 1440 1450
VQGLKKKKVI VIPVGIGPHA SLKQIRLIEK QAPENKAFLL SGVDELEQRR
1460 1470 1480 1490 1500
DEIVSYLCDL APEAPAPTQP PQVAHVTVSP GIAGISSPGP KRKSMVLDVV
1510 1520 1530 1540 1550
FVLEGSDEVG EANFNKSKEF VEEVIQRMDV SPDATRISVL QYSYTVTMEY
1560 1570 1580 1590 1600
AFNGAQSKEE VLRHVREIRY QGGNRTNTGQ ALQYLSEHSF SPSQGDRVEA
1610 1620 1630 1640 1650
PNLVYMVTGN PASDEIKRLP GDIQVVPIGV GPHANMQELE RISRPIAPIF
1660 1670 1680 1690 1700
IRDFETLPRE APDLVLQTCC SKEGLQLPTL PPLPDCSQPL DVVLLLDGSS
1710 1720 1730 1740 1750
SLPESSFDKM KSFAKAFISK ANIGPHLTQV SVIQYGSINT IDVPWNVVQE
1760 1770 1780 1790 1800
KAHLQSLVDL MQQEGGPSQI GDALAFAVRY VTSQIHGARP GASKAVVIII
1810 1820 1830 1840 1850
MDTSLDPVDT AADAARSNRV AVFPVGVGDR YDEAQLRILA GPGASSNVVK
1860 1870 1880 1890 1900
LQQVEDLSTM ATLGNSFFHK LCSGFSGVCV DEDGNEKRPG DVWTLPDQCH
1910 1920 1930 1940 1950
TVTCLANGQT LLQSHRVNCD HGPRPSCANS QSPVRVEETC GCRWTCPCVC
1960 1970 1980 1990 2000
TGSSTRHIVT FDGQNFKLTG SCSYVIFQNK EQDLEVLLHN GACSPGAKQA
2010 2020 2030 2040 2050
CMKSIEIKHA GVSAELHSNM EMAVDGRLVL APYVGENMEV SIYGAIMYEV
2060 2070 2080 2090 2100
RFTHLGHILT YTPQNNEFQL QLSPKTFASK MHGLCGICDE NGANDFTLRD
2110 2120 2130 2140 2150
GTVTTDWKRL VQEWTVQQPG YTCQAVPEEQ CPVSDSSHCQ VLLSASFAEC
2160 2170 2180 2190 2200
HKVIAPATFH TICQQDSCHQ ERVCEVIASY AHLCRTSGVC VDWRTTDFCA
2210 2220 2230 2240 2250
MSCPPSLVYN HCERGCPRHC DGNTSFCGDH PSEGCFCPQH QVFLEGSCVP
2260 2270 2280 2290 2300
EEACTQCVGE DGVRHQFLET WVPDHQPCQI CMCLSGRKIN CTAQPCPTAR
2310 2320 2330 2340 2350
APTCGPCEVA RLKQSTNLCC PEYECVCDLF NCNLPPVPPC EGGLQPTLTN
2360 2370 2380 2390 2400
PGECRPTFTC DCRKEECKRV SPPSCPPHRT PTLRKTQCCD EYECACSCVN
2410 2420 2430 2440 2450
STLSCPLGYL ASATTNDCGC TTTTCLPDKV CVHRGTVYPV GQFWEEGCDT
2460 2470 2480 2490 2500
CTCTDMEDTV VGLRVVQCSQ RPCEDSCQPG FSYVLHEGEC CGRCLPSACK
2510 2520 2530 2540 2550
VVAGSLRGDS HSSWKSVGSR WAVPENPCLV NECVRVEDAV FVQQRNISCP
2560 2570 2580 2590 2600
QLAVPTCPTG FQLNCETSEC CPSCHCEPVE ACLLNGTIIG PGKSVMVDLC
2610 2620 2630 2640 2650
TTCRCIVQTD AISRFKLECR KTTCEACPMG YREEKSQGEC CGRCLPTACT
2660 2670 2680 2690 2700
IQLRGGRIMT LKQDETFQDG CDSHLCRVNE RGEYIWEKRV TGCPPFDEHK
2710 2720 2730 2740 2750
CLAEGGKIVK IPGTCCDTCE EPDCKDITAK VQYIKVGDCK SQEEVDIHYC
2760 2770 2780 2790 2800
QGKCASKAVY SIDIEDVQEQ CSCCLPSRTE PMRVPLHCTN GSVVYHEVIN
2810 2813
AMQCRCSPRN CSK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3613 Collapse assay details

Data source: Panorama

Official Gene Symbol
Vwf
Peptide Sequence
LSSETFGFAAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
129
Peptide End
139
CPTAC ID
non-CPTAC-3613
Peptide Molecular Mass
1,184.5826
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 24.3 8.5 4.7 27.6 9.6 5.2 36.8 12.8 7 15 15 15
y5 (1+) 14 4 4.5 16.3 8.8 5 21.5 9.7 6.7 15 15 15
y6 (1+) 8.8 5.9 4.4 11.2 8.3 8.6 14.2 10.2 9.7 15 15 15
y7 (1+) 25 7.6 5.5 17.1 11.4 8.6 30.3 13.7 10.2 15 15 15
y9 (1+) 8.1 6.7 4.8 12.2 10.7 9.3 14.6 12.6 10.5 15 15 15
sum 6.7 2.4 1.2 9.5 7.1 4.6 11.6 7.5 4.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments