Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Sparcl1 Ecm2, Sc1
Sequence Length (AA) Molecular Weight (Da)
650 72287
Protein Name
SPARC-like protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKAVLLLLCA LGTAVAIPTS TRFLSDHSNP TTATLVTPED ATVPIAGVEA
60 70 80 90 100
TADIENHPSD KAEKPSALNS EEETHEQSTE QDKTYSFEVD LKDEEDGDGD
110 120 130 140 150
LSVDPTEGTL TLDLQEGTSE PQQKSLPENG DFPATVSTSY VDPNQRANIT
160 170 180 190 200
KGKESQEQPV SDSHQQPNES SKQTQDLKAE ESQTQDPDIP NEEEEEEEDE
210 220 230 240 250
EEEEEEEPED IGAPSDNQEE GKEPLEEQPT SKWEGNREQS DDTLEESSQP
260 270 280 290 300
TQISKTEKHQ SEQGNQGQES DSEAEGEDKA AGSKEHIPHT EQQDQEGKAG
310 320 330 340 350
LEAIGNQKDT DEKAVSTEPT DAAVVPRSHG GAGDNGGGDD SKHGAGDDYF
360 370 380 390 400
IPSQEFLEAE RMHSLSYYLK YGGGEETTTG ESENRREAAD NQEAKKAESS
410 420 430 440 450
PNAEPSDEGN SREHSAGSCT NFQCKRGHIC KTDPQGKPHC VCQDPETCPP
460 470 480 490 500
AKILDQACGT DNQTYASSCH LFATKCRLEG TKKGHQLQLD YFGACKSIPA
510 520 530 540 550
CTDFEVAQFP LRMRDWLKNI LMQLYEPNPK HGGYLNEKQR SKVKKIYLDE
560 570 580 590 600
KRLLAGDHPI ELLLRDFKKN YHMYVYPVHW QFNELDQHPA DRILTHSELA
610 620 630 640 650
PLRASLVPME HCITRFFEEC DPNKDKHITL KEWGHCFGIK EEDIDENLLF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-3616 Collapse assay details

Data source: Panorama

Official Gene Symbol
Sparcl1
Peptide Sequence
LLAGDHPIELLLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
553
Peptide End
565
CPTAC ID
non-CPTAC-3616
Peptide Molecular Mass
1,458.8558
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 4 1.5 1.6 5.5 7.5 5.3 6.8 7.6 5.5 15 15 15
y10 (2+) 17.5 2.4 1.4 32.7 6.8 5.4 37.1 7.2 5.6 15 15 15
y11 (2+) 3.2 1.2 1.6 5.6 6.1 5.9 6.4 6.2 6.1 15 15 15
y12 (2+) 9.2 2.2 2.2 18.2 6.4 4.2 20.4 6.8 4.7 15 15 15
y7 (1+) 3.7 1.6 1.4 6 6.8 7.6 7 7 7.7 15 15 15
sum 2.4 0.8 1.2 4.7 6.2 5.6 5.3 6.3 5.7 15 15 15


Additional Resources and Comments