Sparcl1, CPTAC-3616 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Sparcl1	Ecm2, Sc1

Sequence Length (AA)	Molecular Weight (Da)
650	72287

Protein Name
SPARC-like protein 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKAVLLLLCA	LGTAVAIPTS	TRFLSDHSNP	TTATLVTPED	ATVPIAGVEA
60	70	80	90	100
TADIENHPSD	KAEKPSALNS	EEETHEQSTE	QDKTYSFEVD	LKDEEDGDGD
110	120	130	140	150
LSVDPTEGTL	TLDLQEGTSE	PQQKSLPENG	DFPATVSTSY	VDPNQRANIT
160	170	180	190	200
KGKESQEQPV	SDSHQQPNES	SKQTQDLKAE	ESQTQDPDIP	NEEEEEEEDE
210	220	230	240	250
EEEEEEEPED	IGAPSDNQEE	GKEPLEEQPT	SKWEGNREQS	DDTLEESSQP
260	270	280	290	300
TQISKTEKHQ	SEQGNQGQES	DSEAEGEDKA	AGSKEHIPHT	EQQDQEGKAG
310	320	330	340	350
LEAIGNQKDT	DEKAVSTEPT	DAAVVPRSHG	GAGDNGGGDD	SKHGAGDDYF
360	370	380	390	400
IPSQEFLEAE	RMHSLSYYLK	YGGGEETTTG	ESENRREAAD	NQEAKKAESS
410	420	430	440	450
PNAEPSDEGN	SREHSAGSCT	NFQCKRGHIC	KTDPQGKPHC	VCQDPETCPP
460	470	480	490	500
AKILDQACGT	DNQTYASSCH	LFATKCRLEG	TKKGHQLQLD	YFGACKSIPA
510	520	530	540	550
CTDFEVAQFP	LRMRDWLKNI	LMQLYEPNPK	HGGYLNEKQR	SKVKKIYLDE
560	570	580	590	600
KRLLAGDHPI	ELLLRDFKKN	YHMYVYPVHW	QFNELDQHPA	DRILTHSELA
610	620	630	640	650
PLRASLVPME	HCITRFFEEC	DPNKDKHITL	KEWGHCFGIK	EEDIDENLLF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3616 Collapse assay details

Data source: Panorama

Official Gene Symbol: Sparcl1
Peptide Sequence: LLAGDHPIELLLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 553
Peptide End: 565

CPTAC ID: non-CPTAC-3616
Peptide Molecular Mass: 1,458.8558
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	4	1.5	1.6	5.5	7.5	5.3	6.8	7.6	5.5	15	15	15
y10 (2+)	17.5	2.4	1.4	32.7	6.8	5.4	37.1	7.2	5.6	15	15	15
y11 (2+)	3.2	1.2	1.6	5.6	6.1	5.9	6.4	6.2	6.1	15	15	15
y12 (2+)	9.2	2.2	2.2	18.2	6.4	4.2	20.4	6.8	4.7	15	15	15
y7 (1+)	3.7	1.6	1.4	6	6.8	7.6	7	7	7.7	15	15	15
sum	2.4	0.8	1.2	4.7	6.2	5.6	5.3	6.3	5.7	15	15	15

Sparcl1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3616 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Sparcl1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3616 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments