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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Tie1 Tie, Tie-1
Sequence Length (AA) Molecular Weight (Da)
1134 124583
Protein Name
Tyrosine-protein kinase receptor Tie-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVWWGSSLLL PTLFLASHVG ASVDLTLLAN LRITDPQRFF LTCVSGEAGA
60 70 80 90 100
GRSSDPPLLL EKDDRIVRTF PPGQPLYLAR NGSHQVTLRG FSKPSDLVGV
110 120 130 140 150
FSCVGGAGAR RTRVLYVHNS PGAHLFPDKV THTVNKGDTA VLSAHVHKEK
160 170 180 190 200
QTDVIWKNNG SYFNTLDWQE ADDGRFQLQL QNVQPPSSGI YSATYLEASP
210 220 230 240 250
LGSAFFRLIV RGCGAGRWGP GCVKDCPGCL HGGVCHDHDG ECVCPPGFTG
260 270 280 290 300
TRCEQACREG RFGQSCQEQC PGTAGCRGLT FCLPDPYGCS CGSGWRGSQC
310 320 330 340 350
QEACAPGHFG ADCRLQCQCQ NGGTCDRFSG CVCPSGWHGV HCEKSDRIPQ
360 370 380 390 400
ILSMATEVEF NIGTMPRINC AAAGNPFPVR GSMKLRKPDG TMLLSTKVIV
410 420 430 440 450
EPDRTTAEFE VPSLTLGDSG FWECRVSTSG GQDSRRFKVN VKVPPVPLTA
460 470 480 490 500
PRLLAKQSRQ LVVSPLVSFS GDGPISSVRL HYRPQDSTIA WSAIVVDPSE
510 520 530 540 550
NVTLMNLKPK TGYNVRVQLS RPGEGGEGGW GPSALMTTDC PEPLLQPWLE
560 570 580 590 600
SWHVEGPDRL RVSWSLPSVP LSGDGFLLRL WDGARGQERR ENISFPQART
610 620 630 640 650
ALLTGLTPGT HYQLDVRLYH CTLLGPASPP AHVHLPPSGP PAPRHLHAQA
660 670 680 690 700
LSDSEIQLMW QHPEAPSGPI SKYIVEIQVA GGSGDPQWMD VDRPEETSII
710 720 730 740 750
VRGLNASTRY LFRVRASVQG LGDWSNTVEE ATLGNGLQSE GPVRESRAAE
760 770 780 790 800
EGLDQQLVLA VVGSVSATCL TILAALLALV CIRRSCLHRR RTFTYQSGSG
810 820 830 840 850
EETILQFSSG TLTLTRRPKP QPEPLSYPVL EWEDITFEDL IGEGNFGQVI
860 870 880 890 900
RAMIKKDGLK MNAAIKMLKE YASENDHRDF AGELEVLCKL GHHPNIINLL
910 920 930 940 950
GACENRGYLY IAIEYAPYGN LLDFLRKSRV LETDPAFARE HGTASTLSSR
960 970 980 990 1000
QLLRFASDAA NGMQYLSEKQ FIHRDLAARN VLVGENLASK IADFGLSRGE
1010 1020 1030 1040 1050
EVYVKKTMGR LPVRWMAIES LNYSVYTTKS DVWSFGVLLW EIVSLGGTPY
1060 1070 1080 1090 1100
CGMTCAELYE KLPQGYRMEQ PRNCDDEVYE LMRQCWRDRP YERPPFAQIA
1110 1120 1130 1134
LQLGRMLEAR KAYVNMSLFE NFTYAGIDAT AEEA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3623 Collapse assay details

Data source: Panorama

Official Gene Symbol
Tie1
Peptide Sequence
VLYVHNSPGAHLFPDK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
114
Peptide End
129
CPTAC ID
non-CPTAC-3623
Peptide Molecular Mass
1,792.9261
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 19.4 9.2 4.9 20.7 11.8 6.5 28.4 15 8.1 15 15 15
y12 (3+) 28.4 5.9 5.7 32.8 9.1 5.6 43.4 10.8 8 15 15 15
y14 (3+) 10.4 6.1 5.1 14.1 9.7 6.9 17.5 11.5 8.6 15 15 15
y15 (3+) 11.4 4.3 2.6 12.7 6.6 3.8 17.1 7.9 4.6 15 15 15
y3 (1+) 14.5 5 5.1 20.1 9.6 7.8 24.8 10.8 9.3 15 15 15
sum 8.7 3.9 3.3 12.4 7.9 5.2 15.1 8.8 6.2 15 15 15


Additional Resources and Comments