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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Akap12

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Akap12 Gag12, Ssecks
Sequence Length (AA) Molecular Weight (Da)
1684 180695
Protein Name
A-kinase anchor protein 12
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGAGSSTEQR SPEQPAESDT PSELELSGHG PAAEASGAAG DPADADPATK
60 70 80 90 100
LPQKNGQLSA VNGVAEQEDV HVQEESQDGQ EEEVTVEDVG QRESEDVKEK
110 120 130 140 150
DRAKEMAASS TVVEDITKDE QEETPEIIEQ IPASESNVEE MAQAAESQAN
160 170 180 190 200
DVGFKKVFKF VGFKFTVKKD KNEKSDTVQL LTVKKDEGEG AEASVGAGDH
210 220 230 240 250
QEPGVETVGE SASKESELKQ STEKQEGTLK QAQSSTEIPL QAESGQGTEE
260 270 280 290 300
EAAKDGEENR EKEPTKPLES PTSPVSNETT SSFKKFFTHG WAGWRKKTSF
310 320 330 340 350
KKPKEDDLET SEKRKEQEAE KVDEEEGEKT EPAPAEEQEP AEGTDQARLS
360 370 380 390 400
ADYEKVELPL EDQVGDLEAL SEKCAPLATE VFDEKTEAHQ EVVAEVHVST
410 420 430 440 450
VEKMTKGQGG AEVEGDVVVE GSGESLPPEK LAETQEVPQE AEPVEELMKT
460 470 480 490 500
KEVCVSGGDH TQLTDLSPEE KMLPKHPEGI VSEVEMLSSQ ERIKVQGSPL
510 520 530 540 550
KKLFSSSGLK KLSGKKQKGK RGGGGGDEEP GEYQHIQTES PESADEQKGE
560 570 580 590 600
SSASSPEEPE EIACLEKGPS EAPQEAEAEE GATSDGEKKR EGITPWASFK
610 620 630 640 650
KMVTPKKRVR RPSESDKEEE LDKVKSATLS STESTASGMQ DEVRAVGEEQ
660 670 680 690 700
RSEEPKRRVD TSVSWEALIC VGSSKKRARK ASSSDDEGGP RTLGGDGHRA
710 720 730 740 750
EEASKDKEAD ALPASTQEQD QAHGSSSPEP AGSPSEGEGV STWESFKRLV
760 770 780 790 800
TPRKKSKSKL EERAEDSGAE QLASEIEPSR EESWVSIKKF IPGRRKKRAD
810 820 830 840 850
GKQEQAAVED SGPGEINEDD PDVPAVVPLS EYDAVEREKL EAQRAQENVE
860 870 880 890 900
LPQLKGAVYV SEELSKTLVH TVSVAVIDGT RAVTSAEERS PSWISASMTE
910 920 930 940 950
PLEHAEGVAT PPVGEVTEKD ITAEATPALA QTLPGGKDAH DDIVTSEVDF
960 970 980 990 1000
TSEAVTAAET TEALRAEELT EASGAEETTD MVSAVSQLSD SPDTTEEATP
1010 1020 1030 1040 1050
VQEVEGGMLD TEEQERQTQA VLQAVADKVK EDSQVPATQT LQRAGPKALE
1060 1070 1080 1090 1100
KVEEVEEDSE VLATEKEKDV VPEGPVQEAE TEHLAQGSET VQATPESLEV
1110 1120 1130 1140 1150
PEVTEDVDRA TTCQVIKHQQ LMEQAVAPES SETLTDSETN GSTPLADSDT
1160 1170 1180 1190 1200
PNGTQQDETV DSQDSNAIAA VKQSQVTEEE AAAAQTEGPS TPSSFPAQEE
1210 1220 1230 1240 1250
HREKPGRDVL EPTQALAAGA VPILAKAEVG QEGEAGQFDG EKVKDGQCVK
1260 1270 1280 1290 1300
ELEVPVHTGP NSQKTADLTR DSEVMEVARC QETESNEEQS ISPEKREMGT
1310 1320 1330 1340 1350
DVEKEETETK TEQASEEHEQ ETAAPEHEGT HPKPVLTADM PHSERGKALG
1360 1370 1380 1390 1400
SLEGSPSLPD QDKADCIEVQ VQSSDTPVTQ TTEAVKKVEE TVATSEMDES
1410 1420 1430 1440 1450
LECAGAQSLP AEKLSETGGY GTLQHGEDTV PQGPESQAES IPIIVTPAPE
1460 1470 1480 1490 1500
SILHSDLQRE VSASQKQRSD EDNKPDAGPD AAGKESAARE KILRAEPEIL
1510 1520 1530 1540 1550
ELESKSNKIV QSVIQTAVDQ FARTETAPET HASDLQNQVP VMQADSQGAQ
1560 1570 1580 1590 1600
QMLDKDESDL QVSPQDGTLS AVAQEGLAVS DSSEGMSKAS EMITTLAVES
1610 1620 1630 1640 1650
ASVKESVEKL PLQCKDEKEH AADGPQHQSL AKAEADASGN LTKESPDTNG
1660 1670 1680 1684
PKLTEEGDAL KEEMNKAQTE EDDLQEPKGD LTES

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3631 Collapse assay details

Data source: Panorama

Official Gene Symbol
Akap12
Peptide Sequence
GAVYVSEELSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
856
Peptide End
866
CPTAC ID
non-CPTAC-3631
Peptide Molecular Mass
1,180.5976
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 12 5.3 4.3 14.1 7.5 5.6 18.5 9.2 7.1 15 15 15
y9 (2+) 24.4 7.7 6.4 24.5 11 10.6 34.6 13.4 12.4 15 15 15
y6 (1+) 7.6 5.3 4.3 9.4 5 5.4 12.1 7.3 6.9 15 15 15
y7 (1+) 15 9.4 8.2 24.1 8.8 8.7 28.4 12.9 12 15 15 15
y8 (1+) 6.7 6 7.9 10.4 7.8 7.3 12.4 9.8 10.8 15 15 15
sum 5 2.4 2.9 9.9 4.6 4.2 11.1 5.2 5.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments