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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Fuca2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Fuca2 N/A
Sequence Length (AA) Molecular Weight (Da)
461 53645
Protein Name
Plasma alpha-L-fucosidase
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRLGFLMLLP LLLLPLLRPW GVTRALSYDP TWESLDRRPL PAWFDQAKFG
60 70 80 90 100
IFIHWGVFSV PSFGSEWFWW YWQKEKKPQF VDFMNNNYAP GFKYEDFVVL
110 120 130 140 150
FTAKYFNANQ WADILQASGA KYVVFTSKHH EGFTMWGSDR SWNWNAVDEG
160 170 180 190 200
PKRDIVKELE VAVRNRTGLH FGLYYSLFEW FHPLFLEDQS SSFQKQRFPV
210 220 230 240 250
SKTLPELYEL VNRYQPEVLW SDGDGGAPDH YWNSTGFLAW LYNESPVRKT
260 270 280 290 300
VVTNDRWGVG SICKHGGYYT CSDRYNPGYL LPHKWENCMT IDKFSWGYRR
310 320 330 340 350
EAEISDYLTI EELVKKLVET VACGGNLLMN IGPTGDGTIP VIFEERLRQM
360 370 380 390 400
GTWLKVNGEA IYETHTWRSQ NDTVTPDVWY TSKPEKKLVY AIFLKWPISG
410 420 430 440 450
KLFLGQPIGS LGETEVELLG HWQPLTWTSS QPSGITVELP LLSVHQMPCK
460 461
WGWTLVLSNV I

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3646 Collapse assay details

Data source: Panorama

Official Gene Symbol
Fuca2
Peptide Sequence
TLPELYELVNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
203
Peptide End
213
CPTAC ID
non-CPTAC-3646
Peptide Molecular Mass
1,345.7242
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 18 7.5 5.2 24.3 6.1 4.7 30.2 9.7 7 15 15 15
y9 (2+) 5.5 3.1 3.8 9.9 5.8 5 11.3 6.6 6.3 15 15 15
y6 (1+) 29.2 7.8 4.6 24.2 11.3 4.3 37.9 13.7 6.3 15 15 15
y7 (1+) 13.7 5.3 4.2 15.9 4.1 5.1 21 6.7 6.6 15 15 15
y9 (1+) 14.8 5 6.6 18 8.5 6.2 23.3 9.9 9.1 15 15 15
sum 3.7 2.5 3 9.8 5.2 4.4 10.5 5.8 5.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments