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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Pkm Pk3, Pkm2, Pykm
Sequence Length (AA) Molecular Weight (Da)
531 57845
Protein Name
Pyruvate kinase PKM
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPKPHSEAGT AFIQTQQLHA AMADTFLEHM CRLDIDSAPI TARNTGIICT
60 70 80 90 100
IGPASRSVEM LKEMIKSGMN VARLNFSHGT HEYHAETIKN VREATESFAS
110 120 130 140 150
DPILYRPVAV ALDTKGPEIR TGLIKGSGTA EVELKKGATL KITLDNAYME
160 170 180 190 200
KCDENILWLD YKNICKVVEV GSKIYVDDGL ISLQVKEKGA DFLVTEVENG
210 220 230 240 250
GSLGSKKGVN LPGAAVDLPA VSEKDIQDLK FGVEQDVDMV FASFIRKAAD
260 270 280 290 300
VHEVRKVLGE KGKNIKIISK IENHEGVRRF DEILEASDGI MVARGDLGIE
310 320 330 340 350
IPAEKVFLAQ KMMIGRCNRA GKPVICATQM LESMIKKPRP TRAEGSDVAN
360 370 380 390 400
AVLDGADCIM LSGETAKGDY PLEAVRMQHL IAREAEAAIY HLQLFEELRR
410 420 430 440 450
LAPITSDPTE AAAVGAVEAS FKCCSGAIIV LTKSGRSAHQ VARYRPRAPI
460 470 480 490 500
IAVTRNPQTA RQAHLYRGIF PVLCKDAVLN AWAEDVDLRV NLAMDVGKAR
510 520 530 531
GFFKKGDVVI VLTGWRPGSG FTNTMRVVPV P

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3654 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pkm
Peptide Sequence
GDLGIEIPAEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
295
Peptide End
305
CPTAC ID
non-CPTAC-3654
Peptide Molecular Mass
1,140.6027
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 10.1 6.5 9.5 25.8 7.5 10.5 27.7 9.9 14.2 15 15 15
y9 (2+) 12.3 7.7 9.5 21.3 10.5 12.4 24.6 13 15.6 15 15 15
y4 (1+) 3 8.2 5.9 19.8 20.8 18.5 20 22.4 19.4 15 15 15
y6 (1+) 8.2 6.6 4.6 16.9 18.7 14.4 18.8 19.8 15.1 15 15 15
y8 (1+) 5.1 7.6 4.7 17.6 6.2 8.6 18.3 9.8 9.8 15 15 15
sum 4.5 4 2.7 20.5 12.8 10.5 21 13.4 10.8 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3588 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pkm
Peptide Sequence
GSGTAEVELK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
126
Peptide End
135
CPTAC ID
non-CPTAC-3588
Peptide Molecular Mass
989.5029
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 8.8 5 6.2 7.5 7.5 8 11.6 9 10.1 15 15 15
y2 (1+) 8.6 6.6 4.9 11.3 7.3 4.9 14.2 9.8 6.9 15 15 15
y5 (1+) 9.2 5.3 4.8 11 7.5 6.7 14.3 9.2 8.2 15 15 15
y6 (1+) 6 4.9 5 8.1 7 6.2 10.1 8.5 8 15 15 15
y8 (1+) 11.7 5.5 6.1 11.9 8.3 7.9 16.7 10 10 15 15 15
sum 4.4 2.4 2.8 5.5 5.1 5 7 5.6 5.7 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2745 Collapse assay details

Data source: Panorama

Official Gene Symbol
PKM
Peptide Modified Sequence
C[+57.0]DENILWLDYK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
N/A
Peptide - Site of Modification
1
Peptide Start
152
Peptide End
162
CPTAC ID
CPTAC-2745
Peptide Molecular Mass
1,467.6704
Species
Homo Sapiens
Assay Type
Direct MRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher Q-Exactive
Internal Standard
13 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 um x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 12.4 5.2 3.8 31.3 6.1 4.4 33.7 8 5.8 15 15 15
y4 (1+) 7.2 5.6 3.5 9.7 5.4 3.6 12.1 7.8 5 15 15 15
b3 (1+) 8 4 3.4 12.3 3.9 3.9 14.7 5.6 5.2 15 15 15
sum 7.2 3.4 3.2 16.8 3.1 3.5 18.3 4.6 4.7 15 15 15


Additional Resources and Comments