Hspa8, CPTAC-3713 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Hspa8	Hsc70, Hsc73

Sequence Length (AA)	Molecular Weight (Da)
646	70871

Protein Name
Heat shock cognate 71 kDa protein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSKGPAVGID	LGTTYSCVGV	FQHGKVEIIA	NDQGNRTTPS	YVAFTDTERL
60	70	80	90	100
IGDAAKNQVA	MNPTNTVFDA	KRLIGRRFDD	AVVQSDMKHW	PFMVVNDAGR
110	120	130	140	150
PKVQVEYKGE	TKSFYPEEVS	SMVLTKMKEI	AEAYLGKTVT	NAVVTVPAYF
160	170	180	190	200
NDSQRQATKD	AGTIAGLNVL	RIINEPTAAA	IAYGLDKKVG	AERNVLIFDL
210	220	230	240	250
GGGTFDVSIL	TIEDGIFEVK	STAGDTHLGG	EDFDNRMVNH	FIAEFKRKHK
260	270	280	290	300
KDISENKRAV	RRLRTACERA	KRTLSSSTQA	SIEIDSLYEG	IDFYTSITRA
310	320	330	340	350
RFEELNADLF	RGTLDPVEKA	LRDAKLDKSQ	IHDIVLVGGS	TRIPKIQKLL
360	370	380	390	400
QDFFNGKELN	KSINPDEAVA	YGAAVQAAIL	SGDKSENVQD	LLLLDVTPLS
410	420	430	440	450
LGIETAGGVM	TVLIKRNTTI	PTKQTQTFTT	YSDNQPGVLI	QVYEGERAMT
460	470	480	490	500
KDNNLLGKFE	LTGIPPAPRG	VPQIEVTFDI	DANGILNVSA	VDKSTGKENK
510	520	530	540	550
ITITNDKGRL	SKEDIERMVQ	EAEKYKAEDE	KQRDKVSSKN	SLESYAFNMK
560	570	580	590	600
ATVEDEKLQG	KINDEDKQKI	LDKCNEIISW	LDKNQTAEKE	EFEHQQKELE
610	620	630	640	646
KVCNPIITKL	YQSAGGMPGG	MPGGFPGGGA	PPSGGASSGP	TIEEVD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3713 Collapse assay details

Data source: Panorama

Official Gene Symbol: Hspa8
Peptide Sequence: DAGTIAGLNVLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 160
Peptide End: 171

CPTAC ID: non-CPTAC-3713
Peptide Molecular Mass: 1,198.6670
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b6 (1+)	15.1	8.5	4.8	21.2	7.2	4.6	26	11.1	6.6	15	15	15
b4 (1+)	16.6	12.2	9.7	20.4	10.6	10.5	26.3	16.2	14.3	15	15	15
y6 (1+)	9.9	9.3	6	12.5	10.8	5.5	15.9	14.3	8.1	15	15	15
y7 (1+)	32	9.4	7.8	32.2	11.9	8.7	45.4	15.2	11.7	15	15	15
y8 (1+)	32.3	9.4	10.1	30.4	10.2	10.5	44.4	13.9	14.6	15	15	15
sum	8.7	4.3	1.9	10.5	4.4	3.1	13.6	6.2	3.6	15	15	15

Hspa8

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3713 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Hspa8

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3713 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments