Serpina3g, CPTAC-3747 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Serpina3g	Spi2A

Sequence Length (AA)	Molecular Weight (Da)
440	49021

Protein Name
Serine protease inhibitor A3G

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGVSPAVFG	CPDVTLGRNT	AVREVQENVT	SVDSLTLVSS	NTDFAFSLYR
60	70	80	90	100
KLVLKNPDEN	VVFSPFSICT	ALALLSLGAK	SNTLKEILEG	LKFNLTETPE
110	120	130	140	150
PDIHQGFRYL	LDLLSQPGNQ	VQISTGSALF	IEKHLQILAE	FKEKARALYQ
160	170	180	190	200
AEAFTADFQQ	PLKATKLIND	YVSNHTQGKI	KELISGLKES	TLMVLVNYIY
210	220	230	240	250
FKGKWKNPFD	PNDTFKSEFY	LDEKRSVIVS	MMKTGYLTTP	YFRDEELSCT
260	270	280	290	300
VVELKYTGNA	SAMFILPDQG	RMQQVEASLQ	PETLRKWKNS	LKPRMIHELR
310	320	330	340	350
LPKFSISTDY	SLEHILPELG	IREVFSTQAD	LSAITGTKDL	RVSQVVHKAV
360	370	380	390	400
LDVAETGTEA	AAATGMAGVG	CCAVFDFLEI	FFNRPFLMII	SDTKAHIALF
410	420	430	440
MAKVTNPERS	MNFPNGEGAS	SQRLESKRLC	FGDPLCLIGQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3747 Collapse assay details

Data source: Panorama

Official Gene Symbol: Serpina3g
Peptide Sequence: ALYQAEAFTADFQQPLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 147
Peptide End: 163

CPTAC ID: non-CPTAC-3747
Peptide Molecular Mass: 1,939.9680
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b7 (1+)	22	17.4	8	31.5	21.2	9.3	38.4	27.4	12.3	15	15	15
b4 (1+)	15.1	10.8	10.4	16.5	14.4	11.4	22.4	18	15.4	15	15	15
y3 (1+)	5.7	5.3	4	12.5	7	5.6	13.7	8.8	6.9	15	15	15
y6 (1+)	13.7	6.2	9.1	11.8	9.8	10	18.1	11.6	13.5	15	15	15
y8 (1+)	20.1	10.3	6.7	17.8	12.5	7.1	26.8	16.2	9.8	15	15	15
sum	6.6	2.9	2.6	10.2	6.9	4.6	12.1	7.5	5.3	15	15	15

Serpina3g

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3747 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Serpina3g

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3747 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments