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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Srsf3 Sfrs3, Srp20, X16
Sequence Length (AA) Molecular Weight (Da)
164 19330
Protein Name
Serine/arginine-rich splicing factor 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MHRDSCPLDC KVYVGNLGNN GNKTELERAF GYYGPLRSVW VARNPPGFAF
60 70 80 90 100
VEFEDPRDAA DAVRELDGRT LCGCRVRVEL SNGEKRSRNR GPPPSWGRRP
110 120 130 140 150
RDDYRRRSPP PRRRSPRRRS FSRSRSRSLS RDRRRERSLS RERNHKPSRS
160 164
FSRSRSRSRS NERK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3749 Collapse assay details

Data source: Panorama

Official Gene Symbol
Srsf3
Peptide Sequence
DAADAVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
58
Peptide End
64
CPTAC ID
non-CPTAC-3749
Peptide Molecular Mass
716.3453
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 17.5 19.1 13.1 19.1 25.4 20.9 25.9 31.8 24.7 15 15 15
y3 (1+) 6.4 5.7 4.3 21 21.5 18.1 22 22.2 18.6 15 15 15
y4 (1+) 5.9 3 5.3 11.8 4.8 5.2 13.2 5.7 7.4 15 15 15
y5 (1+) 3.3 3.5 2.8 9.4 5.6 4.5 10 6.6 5.3 15 15 15
b3 (1+) 7 6.5 6.8 6.7 8.3 8.6 9.7 10.5 11 15 15 15
sum 2.3 1.8 2.2 8.9 5.6 4.2 9.2 5.9 4.7 15 15 15


Additional Resources and Comments