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Overview Data source: UniProt

Official Gene Symbol Other Aliases
RPTOR KIAA1303, RAPTOR
Sequence Length (AA) Molecular Weight (Da)
1335 149038
Protein Name
Regulatory-associated protein of mTOR
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MESEMLQSPL LGLGEEDEAD LTDWNLPLAF MKKRHCEKIE GSKSLAQSWR
60 70 80 90 100
MKDRMKTVSV ALVLCLNVGV DPPDVVKTTP CARLECWIDP LSMGPQKALE
110 120 130 140 150
TIGANLQKQY ENWQPRARYK QSLDPTVDEV KKLCTSLRRN AKEERVLFHY
160 170 180 190 200
NGHGVPRPTV NGEVWVFNKN YTQYIPLSIY DLQTWMGSPS IFVYDCSNAG
210 220 230 240 250
LIVKSFKQFA LQREQELEVA AINPNHPLAQ MPLPPSMKNC IQLAACEATE
260 270 280 290 300
LLPMIPDLPA DLFTSCLTTP IKIALRWFCM QKCVSLVPGV TLDLIEKIPG
310 320 330 340 350
RLNDRRTPLG ELNWIFTAIT DTIAWNVLPR DLFQKLFRQD LLVASLFRNF
360 370 380 390 400
LLAERIMRSY NCTPVSSPRL PPTYMHAMWQ AWDLAVDICL SQLPTIIEEG
410 420 430 440 450
TAFRHSPFFA EQLTAFQVWL TMGVENRNPP EQLPIVLQVL LSQVHRLRAL
460 470 480 490 500
DLLGRFLDLG PWAVSLALSV GIFPYVLKLL QSSARELRPL LVFIWAKILA
510 520 530 540 550
VDSSCQADLV KDNGHKYFLS VLADPYMPAE HRTMTAFILA VIVNSYHTGQ
560 570 580 590 600
EACLQGNLIA ICLEQLNDPH PLLRQWVAIC LGRIWQNFDS ARWCGVRDSA
610 620 630 640 650
HEKLYSLLSD PIPEVRCAAV FALGTFVGNS AERTDHSTTI DHNVAMMLAQ
660 670 680 690 700
LVSDGSPMVR KELVVALSHL VVQYESNFCT VALQFIEEEK NYALPSPATT
710 720 730 740 750
EGGSLTPVRD SPCTPRLRSV SSYGNIRAVA TARSLNKSLQ NLSLTEESGG
760 770 780 790 800
AVAFSPGNLS TSSSASSTLG SPENEEHILS FETIDKMRRA SSYSSLNSLI
810 820 830 840 850
GVSFNSVYTQ IWRVLLHLAA DPYPEVSDVA MKVLNSIAYK ATVNARPQRV
860 870 880 890 900
LDTSSLTQSA PASPTNKGVH IHQAGGSPPA SSTSSSSLTN DVAKQPVSRD
910 920 930 940 950
LPSGRPGTTG PAGAQYTPHS HQFPRTRKMF DKGPEQTADD ADDAAGHKSF
960 970 980 990 1000
ISATVQTGFC DWSARYFAQP VMKIPEEHDL ESQIRKEREW RFLRNSRVRR
1010 1020 1030 1040 1050
QAQQVIQKGI TRLDDQIFLN RNPGVPSVVK FHPFTPCIAV ADKDSICFWD
1060 1070 1080 1090 1100
WEKGEKLDYF HNGNPRYTRV TAMEYLNGQD CSLLLTATDD GAIRVWKNFA
1110 1120 1130 1140 1150
DLEKNPEMVT AWQGLSDMLP TTRGAGMVVD WEQETGLLMS SGDVRIVRIW
1160 1170 1180 1190 1200
DTDREMKVQD IPTGADSCVT SLSCDSHRSL IVAGLGDGSI RVYDRRMALS
1210 1220 1230 1240 1250
ECRVMTYREH TAWVVKASLQ KRPDGHIVSV SVNGDVRIFD PRMPESVNVL
1260 1270 1280 1290 1300
QIVKGLTALD IHPQADLIAC GSVNQFTAIY NSSGELINNI KYYDGFMGQR
1310 1320 1330 1335
VGAISCLAFH PHWPHLAVGS NDYYISVYSV EKRVR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5744 Collapse assay details

Data source: Panorama

Official Gene Symbol
RPTOR
Peptide Sequence
ALETIGANLQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
98
Peptide End
108
CPTAC ID
CPTAC-5744
Peptide Molecular Mass
1,156.6452
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 22.3 11.9 5.3 26.8 26.8 13 34.9 29.3 14 15 15 15
y7 (1+) 26.3 11.2 5.2 29.4 24.8 13.2 39.4 27.2 14.2 15 15 15
y6 (1+) 17.5 10.1 5.8 23.7 25.9 13.8 29.5 27.8 15 15 15 15
sum 18.5 10.4 5.5 24 25.8 13.5 30.3 27.8 14.6 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-5743 Collapse assay details

Data source: Panorama

Official Gene Symbol
RPTOR
Peptide Modified Sequence
SYNC[+57.021464]TPVSSPR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
359
Peptide End
369
CPTAC ID
CPTAC-5743
Peptide Molecular Mass
1,266.5663
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 20.5 12.2 8.6 24.5 32.3 18.5 31.9 34.5 20.4 15 15 15
y8 (1+) 21.2 12.5 8.9 21.8 32.9 18.5 30.4 35.2 20.5 15 15 15
y4 (1+) 18.7 10.8 9.4 22.7 32.4 19.3 29.4 34.2 21.5 15 15 15
y6 (1+) 22.1 11.4 9.1 22.4 32.6 18.1 31.5 34.5 20.3 15 15 15
sum 20.9 11.5 9 22.4 32.5 18.3 30.6 34.5 20.4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5441 Collapse assay details

Data source: Panorama

Official Gene Symbol
RPTOR
Peptide Sequence
ALETIGANLQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
98
Peptide End
108
CPTAC ID
non-CPTAC-5441
Peptide Molecular Mass
1,156.6452
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 11.8 2 2.4 12.3 4.2 2.9 17 4.7 3.8 15 15 15
y6 (1+) 4.2 2.6 2.3 4.7 4.6 2.8 6.3 5.3 3.6 15 15 15
y7 (1+) 6.3 2.7 3.3 8.5 3 3.2 10.6 4 4.6 15 15 15
y8 (1+) 5.4 3.2 2.3 6.9 3.4 2.5 8.8 4.7 3.4 15 15 15
sum 3.1 2 1.6 3.6 3.7 2 4.8 4.2 2.6 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5445 Collapse assay details

Data source: Panorama

Official Gene Symbol
RPTOR
Peptide Sequence
SLIVAGLGDGSIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1179
Peptide End
1191
CPTAC ID
non-CPTAC-5445
Peptide Molecular Mass
1,256.7089
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 9.3 4.3 3.6 14.1 5.6 4.4 16.9 7.1 5.7 15 15 15
y8 (1+) 3.8 2.1 1.7 5.7 4.9 2.4 6.9 5.3 2.9 15 15 15
y9 (1+) 6.3 1.8 0.9 6.4 2.3 2.3 9 2.9 2.5 15 15 15
y10 (1+) 6.3 2.7 1.5 5.3 3.1 2.9 8.2 4.1 3.3 15 15 15
sum 3.8 1.1 0.9 4.8 2.9 1.9 6.1 3.1 2.1 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5442 Collapse assay details

Data source: Panorama

Official Gene Symbol
RPTOR
Peptide Modified Sequence
SYNC[+57.021464]TPVSSPR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
363
Peptide - Site of Modification
4
Peptide Start
359
Peptide End
369
CPTAC ID
non-CPTAC-5442
Peptide Molecular Mass
1,266.5663
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 8 4.8 3.4 9.7 6.1 6.5 12.6 7.8 7.3 15 15 15
y4 (1+) 11.5 3.1 1.9 14.4 4.6 5.7 18.4 5.5 6 15 15 15
y6 (1+) 6.1 1.7 2.8 8.4 2.2 5.6 10.4 2.8 6.3 15 15 15
y7 (1+) 12.6 3.9 3.8 11.2 4.1 6.9 16.9 5.7 7.9 15 15 15
y8 (1+) 8.8 4 5 9 4.3 6.9 12.6 5.9 8.5 15 15 15
sum 5.1 1.8 2.4 5.7 1.9 5.7 7.6 2.6 6.2 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5444 Collapse assay details

Data source: Panorama

Official Gene Symbol
RPTOR
Peptide Sequence
VLDTSSLTQSAPASPTNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
850
Peptide End
867
CPTAC ID
non-CPTAC-5444
Peptide Molecular Mass
1,815.9214
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 11.1 4.5 3.4 9 4.9 4.5 14.3 6.7 5.6 15 15 15
y10 (1+) 13.3 6 3.8 14.4 6.4 3.7 19.6 8.8 5.3 15 15 15
y11 (1+) 11.3 4.3 3.7 17.2 5.5 3.9 20.6 7 5.4 15 15 15
y7 (1+) 3.9 2.8 1.8 5.4 3.5 2.4 6.7 4.5 3 15 15 15
sum 2.9 2.4 1.1 4.1 3.1 2.2 5 3.9 2.5 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5443 Collapse assay details

Data source: Panorama

Official Gene Symbol
RPTOR
Peptide Sequence
VLNSIAYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
833
Peptide End
840
CPTAC ID
non-CPTAC-5443
Peptide Molecular Mass
906.5175
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 3.3 3.3 2.6 4.6 3.2 2.6 5.7 4.6 3.7 15 15 15
y7 (1+) 8 5.8 3.4 7 5.6 4.6 10.6 8.1 5.7 15 15 15
y4 (1+) 14.1 6.8 7.9 22.3 8.3 8.1 26.4 10.7 11.3 15 15 15
y5 (1+) 11.8 4.5 5.5 11.9 6.1 6 16.8 7.6 8.1 15 15 15
sum 2.7 3.2 2.5 4.5 3.2 2.7 5.2 4.5 3.7 15 15 15


Additional Resources and Comments