C1qa, CPTAC-5581 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
C1qa	N/A

Sequence Length (AA)	Molecular Weight (Da)
245	25974

Protein Name
Complement C1q subcomponent subunit A

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
METSQGWLVA	CVLTMTLVWT	VAEDVCRAPN	GKDGAPGNPG	RPGRPGLKGE
60	70	80	90	100
RGEPGAAGIR	TGIRGFKGDP	GESGPPGKPG	NVGLPGPSGP	LGDSGPQGLK
110	120	130	140	150
GVKGNPGNIR	DQPRPAFSAI	RQNPMTLGNV	VIFDKVLTNQ	ESPYQNHTGR
160	170	180	190	200
FICAVPGFYY	FNFQVISKWD	LCLFIKSSSG	GQPRDSLSFS	NTNNKGLFQV
210	220	230	240	245
LAGGTVLQLR	RGDEVWIEKD	PAKGRIYQGT	EADSIFSGFL	IFPSA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-3638 Collapse assay details

Data source: Panorama

Official Gene Symbol: C1qa
Peptide Sequence: DQPRPAFSAIR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 111
Peptide End: 121

CPTAC ID: non-CPTAC-3638
Peptide Molecular Mass: 1,256.6626
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

Repeatability Image Unavailable

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-5581 Collapse assay details

Data source: Panorama

Official Gene Symbol: C1qa
Peptide Sequence: DSLSFSNTNNK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 185
Peptide End: 195

CPTAC ID: non-CPTAC-5581
Peptide Molecular Mass: 1,225.5575
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers