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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Cp N/A
Sequence Length (AA) Molecular Weight (Da)
1061 121151
Protein Name
Ceruloplasmin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKFLLLSTFI FLYSSLALAR DKHYFIGITE AVWDYASGTE EKKLISVDTE
60 70 80 90 100
QSNFYLQNGP DRIGRKYKKA LYFEYTDGTF SKTIDKPAWL GFLGPVIKAE
110 120 130 140 150
VEDKVYVHLK NLASRIYTFH AHGVTYTKEY EGAVYPDNTT DFQRADDKVL
160 170 180 190 200
PGQQYVYVLH ANEPSPGEGD SNCVTRIYHS HVDAPKDIAS GLIGPLILCK
210 220 230 240 250
KGSLYKEKEK NIDQEFVLMF SVVDENLSWY LEDNIKTFCS EPEKVDKDNE
260 270 280 290 300
DFQESNRMYS INGYTFGSLP GLSMCAADRV KWYLFGMGNE VDVHSAFFHG
310 320 330 340 350
QALTSRNYQT DIINLFPATL IDAYMVAQNP GVWMLSCQNL NHLKAGLQAF
360 370 380 390 400
FQVRDCNKPS PEDNIQDRHV RHYYIAAEEV IWNYAPSGTD IFTGENLTAL
410 420 430 440 450
ESDSRVFFEQ GATRIGGSYK KMAYREYTDG SFTNRKQRGP DEEHLGILGP
460 470 480 490 500
VIWAEVGDTI KVTFHNKGQH PLSIQPMGVS FTAENEGTYY GPPGRSSQQA
510 520 530 540 550
ASHVAPKETF TYEWTVPKEM GPTYADPVCL SKMYYSGVDP TKDIFTGLIG
560 570 580 590 600
PMKICKKGSL LADGRQKDVD KEFYLFPTVF DENESLLLDD NIRMFTTAPD
610 620 630 640 650
QVDKEDEDFQ ESNKMHSMNG FMYGNQPGLN MCLGESIVWY LFSAGNEADV
660 670 680 690 700
HGIYFSGNTY LSKGERRDTA NLFPHKSLTL LMNPDTKGTF DVECLTTDHY
710 720 730 740 750
TGGMKQKYTV NQCQRQFEDF TVYLGERTYY VAAVEVEWDY SPSRAWEKEL
760 770 780 790 800
HHLQEQNVSN VFLDKEEFFI GSKYKKVVYR QFTDSSFREQ VKRRAEDEHL
810 820 830 840 850
GILGPPIHAN VGDKVKVVFK NMATRPYSIH AHGVKTESST VVPTLPGEVR
860 870 880 890 900
TYTWQIPERS GAGREDSACI PWAYYSTVDR VKDLYSGLIG PLIVCRKSYV
910 920 930 940 950
KVFSPKKKME FFLLFLVFDE NESWYLDDNI KTYSEHPEKV NKDNEEFLES
960 970 980 990 1000
NKMHAINGKM FGNLQGLTMH VKDEVNWYVM GMGNEIDLHT VHFHGHSFQY
1010 1020 1030 1040 1050
KHRGVYSSDV FDLFPGTYQT LEMFPQTPGT WLLHCHVTDH VHAGMATTYT
1060 1061
VLPVEQETKS G

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-660 Collapse assay details

Data source: Panorama

Official Gene Symbol
CP
Peptide Modified Sequence
EHEGAIYPDN[+1.0]TTDFQR
Modification Type
Deamidated (NQ)
Protein - Site of Modification
138
Peptide - Site of Modification
10
Peptide Start
129
Peptide End
144
CPTAC ID
CPTAC-660
Peptide Molecular Mass
1,892.8176
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 15.2 12.1 12.9 15 10.2 15.2 21.4 15.8 19.9 15 15 15
y7 (1+) 12.6 7.8 10.5 13.6 7.6 13.5 18.5 10.9 17.1 15 15 15
b6 (1+) 13.6 11.1 12.3 15.3 10.5 14.8 20.5 15.3 19.2 15 15 15
sum 13.4 9.9 11.6 14.2 9.1 14.2 19.5 13.4 18.3 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3505 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cp
Peptide Sequence
DIFTGLIGPMK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
543
Peptide End
553
CPTAC ID
non-CPTAC-3505
Peptide Molecular Mass
1,190.6369
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 3.9 2.2 2.3 13.3 3.8 9.6 13.9 4.4 9.9 15 15 15
y8 (1+) 3.1 2.6 2.1 12.1 5.3 6 12.5 5.9 6.4 15 15 15
y9 (1+) 1 0.9 2.2 11.9 2.6 6.7 11.9 2.8 7.1 15 15 15
b2 (1+) 2.7 2.5 1.9 11.7 4.5 6.1 12 5.1 6.4 15 15 15
y9 (2+) 3 2.7 3.5 19 3.6 7.5 19.2 4.5 8.3 15 15 15
sum 1.6 0.8 1.4 12.6 2.9 6.4 12.7 3 6.6 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-1094 Collapse assay details

Data source: Panorama

Official Gene Symbol
CP
Peptide Sequence
GAYPLSIEPIGVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
469
Peptide End
481
CPTAC ID
non-CPTAC-1094
Peptide Molecular Mass
1,370.7558
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (2+) 7.3 3.6 4.7 11.2 6.2 4.6 13.4 7.2 6.6 15 15 15
y11 (2+) 9.5 5.2 5 12 7.3 6.4 15.3 9 8.1 15 15 15
y5 (1+) 5.6 2.8 4.5 12.9 5.5 4.5 14.1 6.2 6.4 15 15 15
y8 (1+) 5.5 4.6 7.1 9.1 5.9 8.5 10.6 7.5 11.1 15 15 15
y10 (1+) 9.2 2.1 4.6 10.9 4.3 3.3 14.3 4.8 5.7 15 15 15
sum 4.5 2.1 3.5 9.7 5.2 3.7 10.7 5.6 5.1 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-1093 Collapse assay details

Data source: Panorama

Official Gene Symbol
CP
Peptide Sequence
IYHSHIDAPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
178
Peptide End
187
CPTAC ID
non-CPTAC-1093
Peptide Molecular Mass
1,179.6037
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (2+) 17 9 7.9 16.7 13.1 9.5 23.8 15.9 12.4 15 15 15
y7 (2+) 6.2 3.5 4.8 10.7 13.8 6.1 12.4 14.2 7.8 15 15 15
y8 (2+) 5.3 3.6 3.4 8 6.4 3.8 9.6 7.3 5.1 15 15 15
y9 (2+) 5.8 3.7 3.8 14.1 9.9 4.6 15.2 10.6 6 15 15 15
y2 (1+) 4.5 4 4 10.4 6.6 5.9 11.3 7.7 7.1 15 15 15
sum 3.2 2.2 2.5 9.3 7 3.3 9.8 7.3 4.1 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5587 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cp
Peptide Sequence
IYHSHVDAPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
177
Peptide End
186
CPTAC ID
non-CPTAC-5587
Peptide Molecular Mass
1,165.5880
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (3+) 18.7 17.2 22.3 25.4 18.9 27 31.5 25.6 35 15 15 15
y8 (2+) 22.4 10.5 8.7 22.1 11.2 8.5 31.5 15.4 12.2 15 15 15
y9 (2+) 12.9 6.5 8 18.3 7.3 11.2 22.4 9.8 13.8 15 15 15
y3 (1+) 11.3 8.6 9.6 14.2 7.8 9.9 18.1 11.6 13.8 15 15 15
y7 (1+) 22.1 14.3 13.9 25.4 12.5 14.4 33.7 19 20 15 15 15
sum 8.9 4.6 5.5 15.2 5.6 7.4 17.6 7.2 9.2 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-1095 Collapse assay details

Data source: Panorama

Official Gene Symbol
CP
Peptide Sequence
NNEGTYYSPNYNPQSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
485
Peptide End
500
CPTAC ID
non-CPTAC-1095
Peptide Molecular Mass
1,902.8133
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 46.2 10.7 8.1 88.4 17 11.2 99.7 20.1 13.8 8 15 14
y8 (2+) 9.6 3.5 4.7 12.9 2.6 7.1 16.1 4.4 8.5 15 15 15
y9 (2+) 19.7 7.6 6.2 18.5 8.8 8 27 11.6 10.1 15 15 15
y4 (1+) 19.8 5.7 5.4 19.8 9.4 5.4 28 11 7.6 15 15 15
y6 (1+) 15.6 8.6 6.4 23 12.2 6.4 27.8 14.9 9.1 13 15 15
sum 8.4 2.6 3.4 17.9 4.4 5.4 19.8 5.1 6.4 15 15 15


Additional Resources and Comments