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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
C1sa C1s
Sequence Length (AA) Molecular Weight (Da)
688 76858
Protein Name
Complement C1s-A subcomponent
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWCLVLFSLL ASFSAEPTMH GEILSPNYPQ AYPNDVVKSW DIEVPEGFGI
60 70 80 90 100
HLYFTHVDIE PSESCAYDSV QIISGGIEEG RLCGQKTSKS PNSPIIEEFQ
110 120 130 140 150
FPYNKLQVVF TSDFSNEERF TGFAAYYTAI DINECTDFTD VPCSHFCNNF
160 170 180 190 200
IGGYFCSCPP EYFLHDDMRN CGVNCSGDVF TALIGEISSP NYPNPYPENS
210 220 230 240 250
RCEYQIQLQE GFQVVVTMQR EDFDVEPADS EGNCPDSLTF ASKNQQFGPY
260 270 280 290 300
CGNGFPGPLT IRTQSNTLGI VFQTDLMGQK KGWKLRYHGD PISCAKKITA
310 320 330 340 350
NSTWEPDKAK YVFKDVVKIT CVDGFEVVEG HVSSTSYYST CQSDGQWSNS
360 370 380 390 400
GLKCQPVYCG IPDPIANGKV EEPENSVFGT VVHYTCEEPY YYMEHEEGGE
410 420 430 440 450
YRCAANGRWV NDQLGIELPR CIPACGVPTE PFQVHQRIFG GQPAKIENFP
460 470 480 490 500
WQVFFNHPRA SGALINEYWV LTAAHVLEKI SDPLMYVGTM SVRTTLLENA
510 520 530 540 550
QRLYSKRVFI HPSWKKEDDP NTRTNFDNDI ALVQLKDPVK MGPKVSPICL
560 570 580 590 600
PGTSSEYNVS PGDMGLISGW GSTEKKVFVI NLRGAKVPVT SLETCKQVKE
610 620 630 640 650
ENPTVRPEDY VFTDNMICAG EKGVDSCHGD SGGAFAFQVP NVTVPKFYVA
660 670 680 688
GLVSWGKRCG TYGVYTKVKN YVDWILKTMQ ENSGPRKD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5597 Collapse assay details

Data source: Panorama

Official Gene Symbol
C1sa
Peptide Sequence
TTLLENAQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
494
Peptide End
502
CPTAC ID
non-CPTAC-5597
Peptide Molecular Mass
1,044.5564
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 8.1 6.5 5.6 9.2 6.2 6.5 12.3 9 8.6 15 15 15
y7 (2+) 11 4.9 4.6 13.2 5.8 8.2 17.2 7.6 9.4 15 15 15
y5 (1+) 8.2 6.4 5.1 12 5.5 6.9 14.5 8.4 8.6 15 15 15
y6 (1+) 9.6 6.5 6.2 15.9 8.1 6.5 18.6 10.4 9 15 15 15
y7 (1+) 15.1 2.7 3.3 16 5.6 3.3 22 6.2 4.7 15 15 15
sum 4.9 1.9 1.8 9.1 4 4.1 10.3 4.4 4.5 15 15 15


Additional Resources and Comments