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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Crtac1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Crtac1 Aspic1, Cep68
Sequence Length (AA) Molecular Weight (Da)
646 70325
Protein Name
Cartilage acidic protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAPSADPGMV RMALLLLPPL WLLPLTGGSQ RAEPMFTAVT NSVLPPDYDS
60 70 80 90 100
NPTQLNYGVA VTDVDHDGDF EIVVAGYTGP NLVLKYNRAQ NRLVNIAVDE
110 120 130 140 150
RSSPYYALRD RQGNAIGVTA CDIDGDGREE IYFLNTNNAF SGVATYTDKL
160 170 180 190 200
FKFRNNRWED ILSDDVNVAR GVASLFAGRS VACVDRTGSG RYSIYIANYA
210 220 230 240 250
YGDVGPDALI EMDPEASDLS RGILALRDVA AEAGVSKYTA GRGVSVGPIL
260 270 280 290 300
SSSASDIFCD NENGPNFLFH NQGNGTFVDT AASAGVDDPH QHGRGVALAD
310 320 330 340 350
FNRDGKVDIV YGNWNGPHRL YLQMSAHGKV RFRDIASPKF STPSPVRTVI
360 370 380 390 400
AADFDNDQEL EVFFNNIAYR SSSANRLFRV IRREHGDPLI EELNPGDALE
410 420 430 440 450
PEGRGTGGVV TDFDGDGMLD LILSHGESMA QPLSVFRGNQ GFSNNWLRVV
460 470 480 490 500
PRTRFGAFAR GAKVVLYTKK SGAHLRIIDG GSGYLCEMEP VAHFGLGRDE
510 520 530 540 550
ASSVEVTWPD GKMVSRSVAS EEMNSVLEIL YPQDEDKLQN TAPLECGQGF
560 570 580 590 600
SQQDNGHCMD TNECIQFPFV CPRDKPVCVN TYGSYRCRTN KRCNRGYEPN
610 620 630 640 646
EDGTACVAQV AFLGGYSSAA FRLSEPLSQA SYLSLGLGLC LQLYAL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5599 Collapse assay details

Data source: Panorama

Official Gene Symbol
Crtac1
Peptide Sequence
GVASLFAGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
171
Peptide End
179
CPTAC ID
non-CPTAC-5599
Peptide Molecular Mass
876.4818
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 18.3 11.5 9.9 19.3 13.8 10.9 26.6 18 14.7 15 15 15
y4 (1+) 8.9 4.7 3.2 11.2 5.4 4.7 14.3 7.2 5.7 15 15 15
y5 (1+) 17.6 6.2 3.7 21.6 6.1 4.3 27.9 8.7 5.7 15 15 15
y6 (1+) 11.5 14.2 5.9 11.7 17.6 7.2 16.4 22.6 9.3 15 15 15
y7 (1+) 8.8 5.3 4.8 14.1 5 6.2 16.6 7.3 7.8 15 15 15
sum 3.8 5.2 3.5 9.4 5.7 4.7 10.1 7.7 5.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments