Apcs, CPTAC-5618 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Apcs	Ptx2, Sap

Sequence Length (AA)	Molecular Weight (Da)
224	26247

Protein Name
Serum amyloid P-component

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MDKLLLWMFV	FTSLLSEAFC	QTDLKRKVFV	FPRESETDHV	KLIPHLEKPL
60	70	80	90	100
QNFTLCFRTY	SDLSRSQSLF	SYSVKGRDNE	LLIYKEKVGE	YSLYIGQSKV
110	120	130	140	150
TVRGMEEYLS	PVHLCTTWES	SSGIVEFWVN	GKPWVKKSLQ	REYTVKAPPS
160	170	180	190	200
IVLGQEQDNY	GGGFQRSQSF	VGEFSDLYMW	DYVLTPQDIL	FVYRDSPVNP
210	220	224
NILNWQALNY	EINGYVVIRP	RVWD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-5618 Collapse assay details

Data source: Panorama

Official Gene Symbol: Apcs
Peptide Sequence: SQSLFSYSVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 66
Peptide End: 75

CPTAC ID: non-CPTAC-5618
Peptide Molecular Mass: 1,144.5764
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b3 (1+)	13.4	3.2	3.3	19.4	9.3	4.2	23.6	9.8	5.3	15	15	15
b2 (1+)	7.5	3.2	2.4	8.6	7.3	5	11.4	8	5.5	15	15	15
y6 (1+)	8.6	2	2.6	11.7	7.3	4	14.5	7.6	4.8	15	15	15
y7 (1+)	18.6	4.5	3.6	27.2	8.5	3.9	33	9.6	5.3	15	15	15
y8 (1+)	6.5	2.8	1.6	11	7.2	3.5	12.8	7.7	3.8	15	15	15
sum	5.7	1.8	0.9	9	6.8	3.5	10.7	7	3.6	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3671 Collapse assay details

Data source: Panorama

Official Gene Symbol: Apcs
Peptide Sequence: VGEYSLYIGQSK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 88
Peptide End: 99

CPTAC ID: non-CPTAC-3671
Peptide Molecular Mass: 1,342.6769
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers