HNRNPL, CPTAC-6096 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
HNRNPL	HNRPL

Sequence Length (AA)	Molecular Weight (Da)
589	64133

Protein Name
Heterogeneous nuclear ribonucleoprotein L

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSRRLLPRAE	KRRRRLEQRQ	QPDEQRRRSG	AMVKMAAAGG	GGGGGRYYGG
60	70	80	90	100
GSEGGRAPKR	LKTDNAGDQH	GGGGGGGGGA	GAAGGGGGGE	NYDDPHKTPA
110	120	130	140	150
SPVVHIRGLI	DGVVEADLVE	ALQEFGPISY	VVVMPKKRQA	LVEFEDVLGA
160	170	180	190	200
CNAVNYAADN	QIYIAGHPAF	VNYSTSQKIS	RPGDSDDSRS	VNSVLLFTIL
210	220	230	240	250
NPIYSITTDV	LYTICNPCGP	VQRIVIFRKN	GVQAMVEFDS	VQSAQRAKAS
260	270	280	290	300
LNGADIYSGC	CTLKIEYAKP	TRLNVFKNDQ	DTWDYTNPNL	SGQGDPGSNP
310	320	330	340	350
NKRQRQPPLL	GDHPAEYGGP	HGGYHSHYHD	EGYGPPPPHY	EGRRMGPPVG
360	370	380	390	400
GHRRGPSRYG	PQYGHPPPPP	PPPEYGPHAD	SPVLMVYGLD	QSKMNCDRVF
410	420	430	440	450
NVFCLYGNVE	KVKFMKSKPG	AAMVEMADGY	AVDRAITHLN	NNFMFGQKLN
460	470	480	490	500
VCVSKQPAIM	PGQSYGLEDG	SCSYKDFSES	RNNRFSTPEQ	AAKNRIQHPS
510	520	530	540	550
NVLHFFNAPL	EVTEENFFEI	CDELGVKRPS	SVKVFSGKSE	RSSSGLLEWE
560	570	580	589
SKSDALETLG	FLNHYQMKNP	NGPYPYTLKL	CFSTAQHAS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-6096 Collapse assay details

Data source: Panorama

Official Gene Symbol: HNRNPL
Peptide Sequence: ISRPGDSDDSR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 179
Peptide End: 189

CPTAC ID: non-CPTAC-6096
Peptide Molecular Mass: 1,203.5480
Species: Homo sapiens (Human)
Assay Type: Direct MRM-MS
Matrix: Bovine Serum Albumin
Submitting Laboratory: McGill University
Submitting Lab PI: Christoph H. Borchers, Gerald Batist

Publication

View Details (opens in a new window)

mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6495B QQQ-MS
Internal Standard: Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Infinity 1290
Column Packing: commercial (Agilent ZORBAX Eclipse Plus C18)
Column Dimensions: 4.6 mm × 150 mm, (5 µm)
Flow Rate: 0.4 mL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b6 (1+)	16.8	4	4.6	21.4	4.6	3.9	27.2	6.1	6	16	16	17
y2 (1+)	14.1	3.8	2.4	19.4	4.5	2.8	24	5.9	3.7	16	16	17
y5 (1+)	18.4	4.1	4.9	22.6	4.6	5.6	29.1	6.2	7.4	16	16	17
y10 (2+)	14.3	2.1	1.5	19.2	2.1	2.2	23.9	3	2.7	16	16	17
y9 (2+)	15.5	4.2	2.4	19.3	3.8	2.5	24.8	5.7	3.5	16	16	17
sum	13.9	2	1.2	18.9	1.8	2	23.5	2.7	2.3	16	16	17

Stability

Data source: Panorama

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Fragment ion / Transition	control_intra_CV	actual_temp_intra_CV	frozen_intra_CV	FTx1_intra_CV	FTx2_intra_CV
b6 (1+)	2.9	3.3	8.1	2.1	8.3
y2 (1+)	5	1.7	1.3	1.1	2.2
y5 (1+)	5.4	4.5	7.6	2.1	2.5
y10 (2+)	1.3	1.4	9.8	2.2	1.5
y9 (2+)	5.1	5.3	6.8	3.6	2.8
sum	1.5	1	7.2	1.9	0.9

Fragment ion / Transition	all_intra_CV	all_inter_CV
b6 (1+)	4.7	6.1
y2 (1+)	2.2	2.4
y5 (1+)	4.4	4.3
y10 (2+)	2.9	5
y9 (2+)	4.8	5.6
sum	2.3	3.7

Endogenous

Data source: Panorama

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Fragment ion / Transition	intra_CV	inter_CV	total_CV
b6 (1+)	36.3	79.5	87.4
y2 (1+)	6.1	15.8	16.9
y5 (1+)	7.5	16.9	18.5
y10 (2+)	8.7	25.3	26.8
y9 (2+)	9.4	31.8	33.2
sum	6.5	18.3	19.4

HNRNPL

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-6096 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Stability

Endogenous

Additional Resources and Comments

Comments

Search form

HNRNPL

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-6096 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Stability

Endogenous

Additional Resources and Comments

Comments