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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

HK1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
HK1 N/A
Sequence Length (AA) Molecular Weight (Da)
917 102486
Protein Name
Hexokinase-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLIDIM TRFRKEMKNG
60 70 80 90 100
LSRDFNPTAT VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE
110 120 130 140 150
KNQNVHMESE VYDTPENIVH GSGSQLFDHV AECLGDFMEK RKIKDKKLPV
160 170 180 190 200
GFTFSFPCQQ SKIDEAILIT WTKRFKASGV EGADVVKLLN KAIKKRGDYD
210 220 230 240 250
ANIVAVVNDT VGTMMTCGYD DQHCEVGLII GTGTNACYME ELRHIDLVEG
260 270 280 290 300
DEGRMCINTE WGAFGDDGSL EDIRTEFDRE IDRGSLNPGK QLFEKMVSGM
310 320 330 340 350
YLGELVRLIL VKMAKEGLLF EGRITPELLT RGKFNTSDVS AIEKNKEGLH
360 370 380 390 400
NAKEILTRLG VEPSDDDCVS VQHVCTIVSF RSANLVAATL GAILNRLRDN
410 420 430 440 450
KGTPRLRTTV GVDGSLYKTH PQYSRRFHKT LRRLVPDSDV RFLLSESGSG
460 470 480 490 500
KGAAMVTAVA YRLAEQHRQI EETLAHFHLT KDMLLEVKKR MRAEMELGLR
510 520 530 540 550
KQTHNNAVVK MLPSFVRRTP DGTENGDFLA LDLGGTNFRV LLVKIRSGKK
560 570 580 590 600
RTVEMHNKIY AIPIEIMQGT GEELFDHIVS CISDFLDYMG IKGPRMPLGF
610 620 630 640 650
TFSFPCQQTS LDAGILITWT KGFKATDCVG HDVVTLLRDA IKRREEFDLD
660 670 680 690 700
VVAVVNDTVG TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMVEGDQ
710 720 730 740 750
GQMCINMEWG AFGDNGCLDD IRTHYDRLVD EYSLNAGKQR YEKMISGMYL
760 770 780 790 800
GEIVRNILID FTKKGFLFRG QISETLKTRG IFETKFLSQI ESDRLALLQV
810 820 830 840 850
RAILQQLGLN STCDDSILVK TVCGVVSRRA AQLCGAGMAA VVDKIRENRG
860 870 880 890 900
LDRLNVTVGV DGTLYKLHPH FSRIMHQTVK ELSPKCNVSF LLSEDGSGKG
910 917
AALITAVGVR LRTEASS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-80 Collapse assay details

Data source: Panorama

Official Gene Symbol
HK1
Peptide Sequence
GDFIALDLGGSSFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
78
Peptide End
91
CPTAC ID
CPTAC-80
Peptide Molecular Mass
1,453.7201
Species
Homo sapiens (Human)
Assay Type
Direct MRM-MS
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 41.8 17.7 4.3 41.3 19.5 26.1 58.8 26.3 26.5 25 15 15
y9 (1+) 41.9 21.8 13.1 47.4 28.9 18.8 63.3 36.2 22.9 25 15 15
y8 (1+) 52.6 14.9 14.8 45.9 24.5 29.6 69.8 28.7 33.1 25 15 15
sum 26.6 14.6 5.5 26 19.5 20.3 37.2 24.4 21 25 15 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-81 Collapse assay details

Data source: Panorama

Official Gene Symbol
HK1
Peptide Sequence
TPDGTENGDFLALDLGGTNFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
519
Peptide End
539
CPTAC ID
CPTAC-81
Peptide Molecular Mass
2,209.0287
Species
Homo sapiens (Human)
Assay Type
Direct MRM-MS
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama

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view larger image

Response Curves

Data source: Panorama

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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 31.8 21.7 30.9 54.8 41.5 41.1 63.4 46.8 51.4 25 15 15
y10 (1+) 47.5 23.1 42.2 53.8 33.4 49.1 71.8 40.6 64.7 25 15 15
y7 (1+) 40.4 18 41.9 46.4 39.6 51.1 61.5 43.5 66.1 25 15 15
sum 20.2 10.4 27.2 31.4 32.7 38.6 37.3 34.3 47.2 25 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
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Comments

Assay Details for non-CPTAC-6095 Collapse assay details

Data source: Panorama

Official Gene Symbol
HK1
Peptide Sequence
GDFIALDLGGSSFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
78
Peptide End
91
CPTAC ID
non-CPTAC-6095
Peptide Molecular Mass
1,453.7201
Species
Homo sapiens (Human)
Assay Type
Direct MRM-MS
Matrix
Bovine Serum Albumin
Submitting Laboratory
McGill University
Submitting Lab PI
Christoph H. Borchers, Gerald Batist

Publication

View Details (opens in a new window)

mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6495B QQQ-MS
Internal Standard
Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Infinity 1290
Column Packing
commercial (Agilent ZORBAX Eclipse Plus C18)
Column Dimensions
4.6 mm × 150 mm, (5 µm)
Flow Rate
0.4 mL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 19.4 10.5 9.4 26.8 10.9 9.7 33.1 15.1 13.5 15 16 17
y10 (1+) 20.2 6.9 10.4 26.4 9.2 9.8 33.2 11.5 14.3 15 16 17
y6 (1+) 17.3 7.8 8.7 20.4 8.6 8.5 26.7 11.6 12.2 15 16 17
y8 (1+) 21 10.6 7.3 31.5 12.5 8.9 37.9 16.4 11.5 15 16 17
y9 (1+) 26 10.4 10.6 28.9 10.6 7.4 38.9 14.8 12.9 15 16 17
sum 15.7 3.5 4.3 21.5 5.4 5.6 26.6 6.4 7.1 15 16 17

Stability

Data source: Panorama

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Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
b3 (1+) 11.1 8.4 11.4 7.2 1.6
y10 (1+) 11.7 13.4 10.3 17.6 4
y6 (1+) 9.7 9.1 13.4 23.9 2.6
y8 (1+) 14.4 12.9 6 11.7 11.6
y9 (1+) 8.8 8.5 10.3 10.3 7.7
sum 6.4 4.3 7.4 11.1 3
Fragment ion / Transition all_intra_CV all_inter_CV
b3 (1+) 8 8.8
y10 (1+) 11.8 7.3
y6 (1+) 11.3 11
y8 (1+) 11.6 11
y9 (1+) 9 10.4
sum 6.1 6.2

Endogenous

Data source: Panorama

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Fragment ion / Transition intra_CV inter_CV total_CV
b3 (1+) 12.3 41.8 43.6
y10 (1+) 54.2 41.2 68.1
y6 (1+) 96.6 40.1 104.6
y8 (1+) 112.9 81.8 139.4
y9 (1+) 15.7 63.5 65.4
sum 36.7 35.5 51.1

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments