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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

ILF3

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ILF3 DRBF, MPHOSPH4, NF90
Sequence Length (AA) Molecular Weight (Da)
894 95338
Protein Name
Interleukin enhancer-binding factor 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRPMRIFVND DRHVMAKHSS VYPTQEELEA VQNMVSHTER ALKAVSDWID
60 70 80 90 100
EQEKGSSEQA ESDNMDVPPE DDSKEGAGEQ KTEHMTRTLR GVMRVGLVAK
110 120 130 140 150
GLLLKGDLDL ELVLLCKEKP TTALLDKVAD NLAIQLAAVT EDKYEILQSV
160 170 180 190 200
DDAAIVIKNT KEPPLSLTIH LTSPVVREEM EKVLAGETLS VNDPPDVLDR
210 220 230 240 250
QKCLAALASL RHAKWFQARA NGLKSCVIVI RVLRDLCTRV PTWGPLRGWP
260 270 280 290 300
LELLCEKSIG TANRPMGAGE ALRRVLECLA SGIVMPDGSG IYDPCEKEAT
310 320 330 340 350
DAIGHLDRQQ REDITQSAQH ALRLAAFGQL HKVLGMDPLP SKMPKKPKNE
360 370 380 390 400
NPVDYTVQIP PSTTYAITPM KRPMEEDGEE KSPSKKKKKI QKKEEKAEPP
410 420 430 440 450
QAMNALMRLN QLKPGLQYKL VSQTGPVHAP IFTMSVEVDG NSFEASGPSK
460 470 480 490 500
KTAKLHVAVK VLQDMGLPTG AEGRDSSKGE DSAEETEAKP AVVAPAPVVE
510 520 530 540 550
AVSTPSAAFP SDATAEQGPI LTKHGKNPVM ELNEKRRGLK YELISETGGS
560 570 580 590 600
HDKRFVMEVE VDGQKFQGAG SNKKVAKAYA ALAALEKLFP DTPLALDANK
610 620 630 640 650
KKRAPVPVRG GPKFAAKPHN PGFGMGGPMH NEVPPPPNLR GRGRGGSIRG
660 670 680 690 700
RGRGRGFGGA NHGGYMNAGA GYGSYGYGGN SATAGYSQFY SNGGHSGNAS
710 720 730 740 750
GGGGGGGGGS SGYGSYYQGD NYNSPVPPKH AGKKQPHGGQ QKPSYGSGYQ
760 770 780 790 800
SHQGQQQSYN QSPYSNYGPP QGKQKGYNHG QGSYSYSNSY NSPGGGGGSD
810 820 830 840 850
YNYESKFNYS GSGGRSGGNS YGSGGASYNP GSHGGYGGGS GGGSSYQGKQ
860 870 880 890 894
GGYSQSNYNS PGSGQNYSGP PSSYQSSQGG YGRNADHSMN YQYR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-395 Collapse assay details

Data source: Panorama

Official Gene Symbol
ILF3
Peptide Sequence
AVSDWIDEQEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
44
Peptide End
54
CPTAC ID
CPTAC-395
Peptide Molecular Mass
1,318.6041
Species
Homo sapiens (Human)
Assay Type
Direct MRM-MS
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 7.4 8.3 4.7 17.1 24 12 18.6 25.4 12.9 25 15 15
y7 (1+) 7.8 9.3 9.5 18.7 25.8 24.1 20.3 27.4 25.9 25 15 15
y6 (1+) 9.9 10.3 7.6 18.7 20.5 13.4 21.2 22.9 15.4 25 15 15
sum 6.4 8.1 5.8 16.8 22.1 13.6 18 23.5 14.8 25 15 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-396 Collapse assay details

Data source: Panorama

Official Gene Symbol
ILF3
Peptide Sequence
VLAGETLSVNDPPDVLDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
183
Peptide End
200
CPTAC ID
CPTAC-396
Peptide Molecular Mass
1,908.9793
Species
Homo sapiens (Human)
Assay Type
Direct MRM-MS
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama

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view larger image

Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 23.7 6.5 4 28.5 14.4 16.9 37.1 15.8 17.4 15 25 15
y10 (1+) 26 11.3 7.1 34.9 15.7 19.8 43.5 19.3 21 15 25 15
y9 (1+) 26.8 10.3 7.5 37.4 15.2 19.5 46 18.4 20.9 15 25 15
sum 15.7 6.9 4.2 27.5 13.8 17.6 31.7 15.4 18.1 15 25 15

Additional Resources and Comments

Resources
Download SOPs
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Assay Details for non-CPTAC-6103 Collapse assay details

Data source: Panorama

Official Gene Symbol
ILF3
Peptide Sequence
IFVNDDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
6
Peptide End
12
CPTAC ID
non-CPTAC-6103
Peptide Molecular Mass
877.4294
Species
Homo sapiens (Human)
Assay Type
Direct MRM-MS
Matrix
Bovine Serum Albumin
Submitting Laboratory
McGill University
Submitting Lab PI
Christoph H. Borchers, Gerald Batist

Publication

View Details (opens in a new window)

mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6495B QQQ-MS
Internal Standard
Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Infinity 1290
Column Packing
commercial (Agilent ZORBAX Eclipse Plus C18)
Column Dimensions
4.6 mm × 150 mm, (5 µm)
Flow Rate
0.4 mL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 2.3 1.6 1.6 2.1 2 1.9 3.1 2.6 2.5 16 16 17
y4 (1+) 3.2 1.7 1.9 3.8 1.6 2.1 5 2.3 2.8 16 16 17
y5 (1+) 1.5 1.9 1.3 1.7 2.3 3.9 2.3 3 4.1 16 16 17
y6 (1+) 1.7 1.4 2.1 2.3 1.1 1.9 2.9 1.8 2.8 16 16 17
y6 (2+) 7 3.9 2.6 6.4 3.7 2.5 9.5 5.4 3.6 16 16 17
sum 0.7 0.9 0.8 1.2 1.2 2.5 1.4 1.5 2.6 16 16 17

Stability

Data source: Panorama

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Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
y2 (1+) 2.6 1.2 2.3 2.4 0.8
y4 (1+) 2 1.5 2.5 0.9 3.5
y5 (1+) 1.5 2.2 2.2 2 1.9
y6 (1+) 1.5 2 2.5 2.3 0.9
y6 (2+) 3.2 3.2 4 3.5 6.8
sum 1 1.3 1.6 0.5 0.6
Fragment ion / Transition all_intra_CV all_inter_CV
y2 (1+) 1.8 1.2
y4 (1+) 2 2.6
y5 (1+) 2 1
y6 (1+) 1.9 1.9
y6 (2+) 4 3.9
sum 1.1 0.6

Endogenous

Data source: Panorama

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Fragment ion / Transition intra_CV inter_CV total_CV
y2 (1+) 0.3 25.7 25.7
y4 (1+) 0.3 27.1 27.1
y5 (1+) 6.8 34.7 35.4
y6 (1+) 9.2 31.4 32.7
y6 (2+) 1.1 25 25
sum 5 31.3 31.7

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments