KRAS, CPTAC-6104 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
KRAS	KRAS2, RASK2

Sequence Length (AA)	Molecular Weight (Da)
189	21656

Protein Name
GTPase KRas

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTEYKLVVVG	AGGVGKSALT	IQLIQNHFVD	EYDPTIEDSY	RKQVVIDGET
60	70	80	90	100
CLLDILDTAG	QEEYSAMRDQ	YMRTGEGFLC	VFAINNTKSF	EDIHHYREQI
110	120	130	140	150
KRVKDSEDVP	MVLVGNKCDL	PSRTVDTKQA	QDLARSYGIP	FIETSAKTRQ
160	170	180	189
RVEDAFYTLV	REIRQYRLKK	ISKEEKTPGC	VKIKKCIIM

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-6104 Collapse assay details

Data source: Panorama

Official Gene Symbol: KRAS
Peptide Sequence: LVVVGAVGVGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 1
Peptide End: 11

CPTAC ID: non-CPTAC-6104
Peptide Molecular Mass: 996.6332
Species: Homo sapiens (Human)
Assay Type: Direct MRM-MS
Matrix: Bovine Serum Albumin
Submitting Laboratory: McGill University
Submitting Lab PI: Christoph H. Borchers, Gerald Batist

Publication

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mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6495B QQQ-MS
Internal Standard: Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Infinity 1290
Column Packing: commercial (Agilent ZORBAX Eclipse Plus C18)
Column Dimensions: 4.6 mm × 150 mm, (5 µm)
Flow Rate: 0.4 mL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	16.7	6.6	4.2	21.1	6.7	5.2	26.9	9.4	6.7	15	16	17
b3 (1+)	16.6	4.8	8.4	21	7.2	7.3	26.8	8.7	11.1	15	16	17
y7 (1+)	19	3.9	6.1	23.2	2.7	5.9	30	4.7	8.5	15	16	17
y8 (1+)	16.1	7	9.2	18	8.3	7.4	24.1	10.9	11.8	15	16	17
y9 (1+)	18.5	7.2	7.9	21.5	6.6	9.6	28.4	9.8	12.4	15	16	17
sum	14.9	1.9	2.4	17.4	1.7	2.6	22.9	2.5	3.5	15	16	17

Stability

Data source: Panorama

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Fragment ion / Transition	control_intra_CV	actual_temp_intra_CV	frozen_intra_CV	FTx1_intra_CV	FTx2_intra_CV
b2 (1+)	8.9	6.3	7.6	7.6	2.7
b3 (1+)	7.2	5.6	5.5	7.5	3.5
y7 (1+)	10.6	6	3.8	2.6	11
y8 (1+)	10	2.9	8.6	10.9	5.3
y9 (1+)	10.9	7.8	4.3	4.7	4.9
sum	5.5	2.3	3.7	2.6	0.9

Fragment ion / Transition	all_intra_CV	all_inter_CV
b2 (1+)	6.6	9.5
b3 (1+)	5.8	9.5
y7 (1+)	6.7	6.6
y8 (1+)	6.8	6.1
y9 (1+)	6.7	6.1
sum	2.9	3.4

Endogenous

Data source: Panorama

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Fragment ion / Transition	intra_CV	inter_CV	total_CV
b2 (1+)	21.4	11.7	24.4
b3 (1+)	0.1	21.7	21.7
y7 (1+)	1.6	11.5	11.6
y8 (1+)	43.4	24.9	50
y9 (1+)	4.7	13.1	13.9
sum	12.6	5.1	13.6

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-1550 Collapse assay details

Data source: Panorama

Official Gene Symbol: KRAS
Peptide Sequence: SFEDIHHYR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 89
Peptide End: 97

CPTAC ID: CPTAC-1550
Peptide Molecular Mass: 1,202.5469
Species: Homo sapiens (Human)
Assay Type: Direct MRM-MS
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu