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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
KRAS KRAS2, RASK2
Sequence Length (AA) Molecular Weight (Da)
189 21656
Protein Name
GTPase KRas
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET
60 70 80 90 100
CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI
110 120 130 140 150
KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QDLARSYGIP FIETSAKTRQ
160 170 180 189
RVEDAFYTLV REIRQYRLKK ISKEEKTPGC VKIKKCIIM

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-6104 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRAS
Peptide Sequence
LVVVGAVGVGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1
Peptide End
11
CPTAC ID
non-CPTAC-6104
Peptide Molecular Mass
996.6332
Species
Homo sapiens (Human)
Assay Type
Direct MRM-MS
Matrix
Bovine Serum Albumin
Submitting Laboratory
McGill University
Submitting Lab PI
Christoph H. Borchers, Gerald Batist

Publication

View Details (opens in a new window)

mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6495B QQQ-MS
Internal Standard
Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Infinity 1290
Column Packing
commercial (Agilent ZORBAX Eclipse Plus C18)
Column Dimensions
4.6 mm × 150 mm, (5 µm)
Flow Rate
0.4 mL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 16.7 6.6 4.2 21.1 6.7 5.2 26.9 9.4 6.7 15 16 17
b3 (1+) 16.6 4.8 8.4 21 7.2 7.3 26.8 8.7 11.1 15 16 17
y7 (1+) 19 3.9 6.1 23.2 2.7 5.9 30 4.7 8.5 15 16 17
y8 (1+) 16.1 7 9.2 18 8.3 7.4 24.1 10.9 11.8 15 16 17
y9 (1+) 18.5 7.2 7.9 21.5 6.6 9.6 28.4 9.8 12.4 15 16 17
sum 14.9 1.9 2.4 17.4 1.7 2.6 22.9 2.5 3.5 15 16 17


Stability

Data source: Panorama

Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
b2 (1+) 8.9 6.3 7.6 7.6 2.7
b3 (1+) 7.2 5.6 5.5 7.5 3.5
y7 (1+) 10.6 6 3.8 2.6 11
y8 (1+) 10 2.9 8.6 10.9 5.3
y9 (1+) 10.9 7.8 4.3 4.7 4.9
sum 5.5 2.3 3.7 2.6 0.9
Fragment ion / Transition all_intra_CV all_inter_CV
b2 (1+) 6.6 9.5
b3 (1+) 5.8 9.5
y7 (1+) 6.7 6.6
y8 (1+) 6.8 6.1
y9 (1+) 6.7 6.1
sum 2.9 3.4

Endogenous

Data source: Panorama

Fragment ion / Transition intra_CV inter_CV total_CV
b2 (1+) 21.4 11.7 24.4
b3 (1+) 0.1 21.7 21.7
y7 (1+) 1.6 11.5 11.6
y8 (1+) 43.4 24.9 50
y9 (1+) 4.7 13.1 13.9
sum 12.6 5.1 13.6

Additional Resources and Comments


Assay Details for CPTAC-1550 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRAS
Peptide Sequence
SFEDIHHYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
89
Peptide End
97
CPTAC ID
CPTAC-1550
Peptide Molecular Mass
1,202.5469
Species
Homo sapiens (Human)
Assay Type
Direct MRM-MS
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 26.4 10.1 13.3 21.4 12.9 14.2 34 16.4 19.5 15 15 15
y6 (1+) 22 11.5 8.5 29.3 16.2 9 36.6 19.9 12.4 15 15 15
y3 (1+) NA NA NA NA NA NA NA NA NA 0 0 0
a2 (1+) 4.4 6.9 3.9 7.3 12.2 7.6 8.5 14 8.5 15 15 15
y4 (1+) NA NA NA NA NA NA NA NA NA 0 0 0
sum 5.1 6 3.4 7.9 11.4 6.9 9.4 12.9 7.7 15 15 15


Additional Resources and Comments