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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
HIST1H4D N/A
Sequence Length (AA) Molecular Weight (Da)
103 11367
Protein Name
Histone H4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSGRGKGGKG LGKGGAKRHR KVLRDNIQGI TKPAIRRLAR RGGVKRISGL
60 70 80 90 100
IYEETRGVLK VFLENVIRDA VTYTEHAKRK TVTAMDVVYA LKRQGRTLYG
103
FGG

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1055 Collapse assay details

Data source: Panorama

Official Gene Symbol
HIST1H4D
Peptide Modified Sequence
GLGK[+42.0]GGAK[+42.0]R
Modification Type
Acetyl (K), Acetyl (K)
Protein - Site of Modification
13, 17
Peptide - Site of Modification
4, 8
Peptide Start
10
Peptide End
18
CPTAC ID
CPTAC-1055
Peptide Molecular Mass
926.5298
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
ovarian tumor tissue
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive mass spectrometer (Thermo Fisher Scientific)
Internal Standard
Heavy-labeled crude peptide (K8, R10)
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 50cm (Analytical)
Flow Rate
250 nL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-B


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 6.1 10.6 8.8 10.2 11.7 11.9 11.9 15.8 14.8 15 15 15
y6 (1+) 14.1 15.4 17.1 14.8 16.3 18.9 20.4 22.4 25.5 15 15 15
y6 (2+) 20.8 20.5 12.7 26.3 19.6 12.6 33.5 28.4 17.9 15 15 15
sum 5.4 10.3 8.9 9.7 11.8 10.8 11.1 15.7 14 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1056 Collapse assay details

Data source: Panorama

Official Gene Symbol
HIST1H4D
Peptide Sequence
VFLENVIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
61
Peptide End
68
CPTAC ID
CPTAC-1056
Peptide Molecular Mass
988.5706
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
ovarian tumor tissue
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive mass spectrometer (Thermo Fisher Scientific)
Internal Standard
Heavy-labeled crude peptide (K8, R10)
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 50cm (Analytical)
Flow Rate
250 nL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-B


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 13.2 6.2 15.9 11.2 7.8 16.1 17.3 10 22.6 15 15 15
y6 (1+) 24.5 16.4 25.6 24.1 25.6 31.7 34.4 30.4 40.7 15 15 15
y5 (1+) 5.5 2.6 6.5 7.5 3.1 6.7 9.3 4 9.3 15 15 15
sum 12.5 8.8 14.4 12.8 11.4 17 17.9 14.4 22.3 15 15 15


Additional Resources and Comments