HIST1H4D, CPTAC-1056 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
HIST1H4D	N/A

Sequence Length (AA)	Molecular Weight (Da)
103	11367

Protein Name
Histone H4

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSGRGKGGKG	LGKGGAKRHR	KVLRDNIQGI	TKPAIRRLAR	RGGVKRISGL
60	70	80	90	100
IYEETRGVLK	VFLENVIRDA	VTYTEHAKRK	TVTAMDVVYA	LKRQGRTLYG
103
FGG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1055 Collapse assay details

Data source: Panorama

Official Gene Symbol: HIST1H4D
Peptide Modified Sequence: GLGK[+42.0]GGAK[+42.0]R
Modification Type: Acetyl (K), Acetyl (K)
Protein - Site of Modification: 13, 17
Peptide - Site of Modification: 4, 8
Peptide Start: 10
Peptide End: 18

CPTAC ID: CPTAC-1055
Peptide Molecular Mass: 926.5298
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: ovarian tumor tissue
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive mass spectrometer (Thermo Fisher Scientific)
Internal Standard: Heavy-labeled crude peptide (K8, R10)
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing: Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions: 300um x 5mm (Trap); 75um x 50cm (Analytical)
Flow Rate: 250 nL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-B

CPTAC-1055:

HIST1H4D.

GLGK[+42.0]GGAK[+42.0]R

CPTAC-1056:

HIST1H4D.

VFLENVIR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	6.1	10.6	8.8	10.2	11.7	11.9	11.9	15.8	14.8	15	15	15
y6 (1+)	14.1	15.4	17.1	14.8	16.3	18.9	20.4	22.4	25.5	15	15	15
y6 (2+)	20.8	20.5	12.7	26.3	19.6	12.6	33.5	28.4	17.9	15	15	15
sum	5.4	10.3	8.9	9.7	11.8	10.8	11.1	15.7	14	15	15	15

Additional Resources and Comments

Resources

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Comments

Assay Details for CPTAC-1056 Collapse assay details

Data source: Panorama

Official Gene Symbol: HIST1H4D
Peptide Sequence: VFLENVIR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 61
Peptide End: 68

CPTAC ID: CPTAC-1056
Peptide Molecular Mass: 988.5706
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: ovarian tumor tissue
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang