ESR1, CPTAC-145 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ESR1	ESR, NR3A1

Sequence Length (AA)	Molecular Weight (Da)
595	66216

Protein Name
Estrogen receptor

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTMTLHTKAS	GMALLHQIQG	NELEPLNRPQ	LKIPLERPLG	EVYLDSSKPA
60	70	80	90	100
VYNYPEGAAY	EFNAAAAANA	QVYGQTGLPY	GPGSEAAAFG	SNGLGGFPPL
110	120	130	140	150
NSVSPSPLML	LHPPPQLSPF	LQPHGQQVPY	YLENEPSGYT	VREAGPPAFY
160	170	180	190	200
RPNSDNRRQG	GRERLASTND	KGSMAMESAK	ETRYCAVCND	YASGYHYGVW
210	220	230	240	250
SCEGCKAFFK	RSIQGHNDYM	CPATNQCTID	KNRRKSCQAC	RLRKCYEVGM
260	270	280	290	300
MKGGIRKDRR	GGRMLKHKRQ	RDDGEGRGEV	GSAGDMRAAN	LWPSPLMIKR
310	320	330	340	350
SKKNSLALSL	TADQMVSALL	DAEPPILYSE	YDPTRPFSEA	SMMGLLTNLA
360	370	380	390	400
DRELVHMINW	AKRVPGFVDL	TLHDQVHLLE	CAWLEILMIG	LVWRSMEHPG
410	420	430	440	450
KLLFAPNLLL	DRNQGKCVEG	MVEIFDMLLA	TSSRFRMMNL	QGEEFVCLKS
460	470	480	490	500
IILLNSGVYT	FLSSTLKSLE	EKDHIHRVLD	KITDTLIHLM	AKAGLTLQQQ
510	520	530	540	550
HQRLAQLLLI	LSHIRHMSNK	GMEHLYSMKC	KNVVPLYDLL	LEMLDAHRLH
560	570	580	590	595
APTSRGGASV	EETDQSHLAT	AGSTSSHSLQ	KYYITGEAEG	FPATV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-145 Collapse assay details

Data source: Panorama

Official Gene Symbol: ESR1
Peptide Sequence: LLFAPNLLLDR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 402
Peptide End: 412

CPTAC ID: CPTAC-145
Peptide Molecular Mass: 1,283.7602
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	15.9	8.4	6.6	35.2	24.8	13.2	38.6	26.2	14.8	15	15	15
y8 (1+)	19.5	10.4	6.4	48.1	25.7	10.5	51.9	27.7	12.3	15	15	15
y7 (1+)	21.9	11.1	10.6	43.6	21.9	14.5	48.8	24.6	18	15	15	15
sum	13.8	9.4	7.2	40.4	22.8	12.5	42.7	24.7	14.4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-1241 Collapse assay details

Data source: Panorama

Official Gene Symbol: ESR1
Peptide Sequence: LLFAPNLLLDR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 402
Peptide End: 412

CPTAC ID: CPTAC-1241
Peptide Molecular Mass: 1,283.7602
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu