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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

ESR1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ESR1 ESR, NR3A1
Sequence Length (AA) Molecular Weight (Da)
595 66216
Protein Name
Estrogen receptor
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTMTLHTKAS GMALLHQIQG NELEPLNRPQ LKIPLERPLG EVYLDSSKPA
60 70 80 90 100
VYNYPEGAAY EFNAAAAANA QVYGQTGLPY GPGSEAAAFG SNGLGGFPPL
110 120 130 140 150
NSVSPSPLML LHPPPQLSPF LQPHGQQVPY YLENEPSGYT VREAGPPAFY
160 170 180 190 200
RPNSDNRRQG GRERLASTND KGSMAMESAK ETRYCAVCND YASGYHYGVW
210 220 230 240 250
SCEGCKAFFK RSIQGHNDYM CPATNQCTID KNRRKSCQAC RLRKCYEVGM
260 270 280 290 300
MKGGIRKDRR GGRMLKHKRQ RDDGEGRGEV GSAGDMRAAN LWPSPLMIKR
310 320 330 340 350
SKKNSLALSL TADQMVSALL DAEPPILYSE YDPTRPFSEA SMMGLLTNLA
360 370 380 390 400
DRELVHMINW AKRVPGFVDL TLHDQVHLLE CAWLEILMIG LVWRSMEHPG
410 420 430 440 450
KLLFAPNLLL DRNQGKCVEG MVEIFDMLLA TSSRFRMMNL QGEEFVCLKS
460 470 480 490 500
IILLNSGVYT FLSSTLKSLE EKDHIHRVLD KITDTLIHLM AKAGLTLQQQ
510 520 530 540 550
HQRLAQLLLI LSHIRHMSNK GMEHLYSMKC KNVVPLYDLL LEMLDAHRLH
560 570 580 590 595
APTSRGGASV EETDQSHLAT AGSTSSHSLQ KYYITGEAEG FPATV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-145 Collapse assay details

Data source: Panorama

Official Gene Symbol
ESR1
Peptide Sequence
LLFAPNLLLDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
402
Peptide End
412
CPTAC ID
CPTAC-145
Peptide Molecular Mass
1,283.7602
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR 		CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR 		CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK 		CPTAC-1223:
MGEA5.
EIPVESIEEVSK 		CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK 		CPTAC-1226:
VAV1.
LNPGDIVELTK 		CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK 		CPTAC-1229:
ADCY9.
TDAHFVDVIK 		CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK 		CPTAC-1232:
B2M.
VEHSDLSFSK 		CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK 		CPTAC-1235:
CALCOCO1.
LQLEGQVTELR 		CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR 		CPTAC-1238:
CDKN1B.
VSNGSPSLER 		CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR 		CPTAC-1241:
ESR1.
LLFAPNLLLDR 		CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR 		CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR 		CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR 		CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR 		CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK 		CPTAC-1250:
IL18.
SDIIFFQR 		CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK 		CPTAC-1253:
CDC25B.
LLGHSPVLR 		CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER 		CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK 		CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK 		CPTAC-1259:
NEK2.
YSDELNEIITR 		CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK 		CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR 		CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK 		CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR 		CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK 		CPTAC-1268:
SMAD4.
GFPHVIYAR 		CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK 		CPTAC-1271:
STAT5A.
HILYNEQR 		CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR 		CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR 		CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK 		CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 15.9 8.4 6.6 35.2 24.8 13.2 38.6 26.2 14.8 15 15 15
y8 (1+) 19.5 10.4 6.4 48.1 25.7 10.5 51.9 27.7 12.3 15 15 15
y7 (1+) 21.9 11.1 10.6 43.6 21.9 14.5 48.8 24.6 18 15 15 15
sum 13.8 9.4 7.2 40.4 22.8 12.5 42.7 24.7 14.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-1241 Collapse assay details

Data source: Panorama

Official Gene Symbol
ESR1
Peptide Sequence
LLFAPNLLLDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
402
Peptide End
412
CPTAC ID
CPTAC-1241
Peptide Molecular Mass
1,283.7602
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR 		CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR 		CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK 		CPTAC-1223:
MGEA5.
EIPVESIEEVSK 		CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK 		CPTAC-1226:
VAV1.
LNPGDIVELTK 		CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK 		CPTAC-1229:
ADCY9.
TDAHFVDVIK 		CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK 		CPTAC-1232:
B2M.
VEHSDLSFSK 		CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK 		CPTAC-1235:
CALCOCO1.
LQLEGQVTELR 		CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR 		CPTAC-1238:
CDKN1B.
VSNGSPSLER 		CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR 		CPTAC-1241:
ESR1.
LLFAPNLLLDR 		CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR 		CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR 		CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR 		CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR 		CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK 		CPTAC-1250:
IL18.
SDIIFFQR 		CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK 		CPTAC-1253:
CDC25B.
LLGHSPVLR 		CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER 		CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK 		CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK 		CPTAC-1259:
NEK2.
YSDELNEIITR 		CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK 		CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR 		CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK 		CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR 		CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK 		CPTAC-1268:
SMAD4.
GFPHVIYAR 		CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK 		CPTAC-1271:
STAT5A.
HILYNEQR 		CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR 		CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR 		CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK 		CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 39.7 26.4 20.9 59.9 25.9 25.4 71.9 37 32.9 15 15 15
y7 (1+) 6.2 6.3 5.3 45 18.6 22.2 45.4 19.6 22.8 15 15 15
y8 (1+) 13.6 11.6 5.9 49 19.9 24.4 50.9 23 25.1 15 15 15
sum 7.2 7.1 5.1 45.5 17.9 22.5 46.1 19.3 23.1 15 15 15

Selectivity

Data source: Panorama

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Slope of Curve Fit for cell line
fragment_ion 1 2 3 4 5 6
y8 (1+) 1.94 3.38 2.61 3.19 5.8 3.1
y7 (1+) 2.38 3.4 2.71 3.11 5.92 3.25
y6 (1+) 1.44 3.31 1.85 1.98 3.74 3.47
sum 2.22 3.39 2.62 3.05 5.76 3.21

Stability

Data source: Panorama

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Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
y7 (1+) 4.5 7.4 4.1 7.5 7.7
y8 (1+) 3 7.6 5.2 3.5 12.7
y6 (1+) 8.5 5.2 5.3 7.1 8.4
sum 3.4 6.5 3.8 5.1 8.2
Fragment ion / Transition all_intra_CV all_inter_CV
y7 (1+) 6.5 6.3
y8 (1+) 6.6 6.6
y6 (1+) 6.6 9.4
sum 5.6 5.6

Endogenous

Data source: Panorama

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Fragment ion / Transition intra_CV inter_CV total_CV
y7 (1+) 77 69.2 103.5
y8 (1+) 57.2 65.5 87
y6 (1+) 68.6 76.7 102.9
sum 59.6 60.2 84.7

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments