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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
GNAI1 N/A
Sequence Length (AA) Molecular Weight (Da)
354 40361
Protein Name
Guanine nucleotide-binding protein G(i) subunit alpha-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGCTLSAEDK AAVERSKMID RNLREDGEKA AREVKLLLLG AGESGKSTIV
60 70 80 90 100
KQMKIIHEAG YSEEECKQYK AVVYSNTIQS IIAIIRAMGR LKIDFGDSAR
110 120 130 140 150
ADDARQLFVL AGAAEEGFMT AELAGVIKRL WKDSGVQACF NRSREYQLND
160 170 180 190 200
SAAYYLNDLD RIAQPNYIPT QQDVLRTRVK TTGIVETHFT FKDLHFKMFD
210 220 230 240 250
VGGQRSERKK WIHCFEGVTA IIFCVALSDY DLVLAEDEEM NRMHESMKLF
260 270 280 290 300
DSICNNKWFT DTSIILFLNK KDLFEEKIKK SPLTICYPEY AGSNTYEEAA
310 320 330 340 350
AYIQCQFEDL NKRKDTKEIY THFTCATDTK NVQFVFDAVT DVIIKNNLKD
354
CGLF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1243 Collapse assay details

Data source: Panorama

Official Gene Symbol
GNAI1
Peptide Modified Sequence
DSGVQAC[+57.0]FNR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
139
Peptide - Site of Modification
7
Peptide Start
133
Peptide End
142
CPTAC ID
CPTAC-1243
Peptide Molecular Mass
1,152.4982
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR
CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR
CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK
CPTAC-1223:
MGEA5.
EIPVESIEEVSK
CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK
CPTAC-1226:
VAV1.
LNPGDIVELTK
CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK
CPTAC-1229:
ADCY9.
TDAHFVDVIK
CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK
CPTAC-1232:
B2M.
VEHSDLSFSK
CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK
CPTAC-1235:
CALCOCO1.
LQLEGQVTELR
CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR
CPTAC-1238:
CDKN1B.
VSNGSPSLER
CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR
CPTAC-1241:
ESR1.
LLFAPNLLLDR
CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR
CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR
CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR
CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR
CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1250:
IL18.
SDIIFFQR
CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK
CPTAC-1253:
CDC25B.
LLGHSPVLR
CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER
CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK
CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK
CPTAC-1259:
NEK2.
YSDELNEIITR
CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK
CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR
CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK
CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR
CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK
CPTAC-1268:
SMAD4.
GFPHVIYAR
CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK
CPTAC-1271:
STAT5A.
HILYNEQR
CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR
CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR
CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK
CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 44.4 11.3 6.6 42.5 11.7 7.9 61.5 16.3 10.3 15 15 15
y5 (1+) 23.2 4.4 5.1 32.5 5.1 6.3 39.9 6.7 8.1 15 15 15
y6 (1+) 26.9 7.9 6.2 54.1 13.1 10.2 60.4 15.3 11.9 15 15 15
sum 24.5 5 3.6 31.7 8.2 6.1 40.1 9.6 7.1 15 15 15

Selectivity

Data source: Panorama

Slope of Curve Fit for cell line
fragment_ion 1 2 3 4 5 6
y4 (1+) 0.11 0.09 0.11 0.09 0.1 0.12
y5 (1+) 0.11 0.1 0.11 0.09 0.1 0.11
y6 (1+) 0.11 0.1 0.1 0.09 0.1 0.1
sum 0.11 0.1 0.1 0.09 0.1 0.11

Stability

Data source: Panorama

Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
Fragment ion / Transition all_intra_CV all_inter_CV

Endogenous

Data source: Panorama

Fragment ion / Transition intra_CV inter_CV total_CV
y4 (1+) 30.7 39.3 49.9
y5 (1+) 28 43.1 51.4
y6 (1+) 25.1 40.4 47.6
sum 23.2 39.3 45.6

Additional Resources and Comments


Assay Details for CPTAC-1244 Collapse assay details

Data source: Panorama

Official Gene Symbol
GNAI1
Peptide Sequence
IAQPNYIPTQQDVLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
162
Peptide End
176
CPTAC ID
CPTAC-1244
Peptide Molecular Mass
1,754.9315
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR
CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR
CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK
CPTAC-1223:
MGEA5.
EIPVESIEEVSK
CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK
CPTAC-1226:
VAV1.
LNPGDIVELTK
CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK
CPTAC-1229:
ADCY9.
TDAHFVDVIK
CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK
CPTAC-1232:
B2M.
VEHSDLSFSK
CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK
CPTAC-1235:
CALCOCO1.
LQLEGQVTELR
CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR
CPTAC-1238:
CDKN1B.
VSNGSPSLER
CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR
CPTAC-1241:
ESR1.
LLFAPNLLLDR
CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR
CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR
CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR
CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR
CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1250:
IL18.
SDIIFFQR
CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK
CPTAC-1253:
CDC25B.
LLGHSPVLR
CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER
CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK
CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK
CPTAC-1259:
NEK2.
YSDELNEIITR
CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK
CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR
CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK
CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR
CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK
CPTAC-1268:
SMAD4.
GFPHVIYAR
CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK
CPTAC-1271:
STAT5A.
HILYNEQR
CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR
CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR
CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK
CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 8.5 4.6 5.4 14.2 9.6 14.9 16.5 10.6 15.8 15 15 15
y9 (1+) 21 4.7 6.1 21.4 11.5 13.5 30 12.4 14.8 15 15 15
y12 (1+) 25.3 7 6.1 25.4 11.8 13.1 35.9 13.7 14.5 15 15 15
sum 5.5 3.7 4.7 12 9.4 14.1 13.2 10.1 14.9 15 15 15

Selectivity

Data source: Panorama

Slope of Curve Fit for cell line
fragment_ion 1 2 3 4 5 6
y12 (1+) 0.42 0.43 0.45 0.41 0.54 0.46
y9 (1+) 0.44 0.44 0.46 0.41 0.46 0.48
y8 (1+) 0.53 0.52 0.54 0.5 0.56 0.58
sum 0.5 0.5 0.51 0.48 0.55 0.55

Stability

Data source: Panorama

Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
y12 (1+) 9.8 13.3 5.4 7.8 6.5
y9 (1+) 6.8 5.9 7.9 4 5.6
y8 (1+) 8 4 1.7 3.7 4.2
sum 6.3 3.1 1.1 2.9 2.8
Fragment ion / Transition all_intra_CV all_inter_CV
y12 (1+) 9.3 9.2
y9 (1+) 6 8.8
y8 (1+) 4.3 6
sum 3.2 5.3

Endogenous

Data source: Panorama

Fragment ion / Transition intra_CV inter_CV total_CV
y12 (1+) 26.3 37.2 45.6
y9 (1+) 28 43.9 52.1
y8 (1+) 27.9 45.9 53.7
sum 25.9 43.2 50.4

Additional Resources and Comments