Overview Data source: UniProt

Official Gene Symbol Other Aliases
SMAD4 DPC4,MADH4
Sequence Length (AA) Molecular Weight (Da)
552 60439
Protein Name
Mothers against decapentaplegic homolog 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDNMSITNTP TSNDACLSIV HSLMCHRQGG ESETFAKRAI ESLVKKLKEK
60 70 80 90 100
KDELDSLITA ITTNGAHPSK CVTIQRTLDG RLQVAGRKGF PHVIYARLWR
110 120 130 140 150
WPDLHKNELK HVKYCQYAFD LKCDSVCVNP YHYERVVSPG IDLSGLTLQS
160 170 180 190 200
NAPSSMMVKD EYVHDFEGQP SLSTEGHSIQ TIQHPPSNRA STETYSTPAL
210 220 230 240 250
LAPSESNATS TANFPNIPVA STSQPASILG GSHSEGLLQI ASGPQPGQQQ
260 270 280 290 300
NGFTGQPATY HHNSTTTWTG SRTAPYTPNL PHHQNGHLQH HPPMPPHPGH
310 320 330 340 350
YWPVHNELAF QPPISNHPAP EYWCSIAYFE MDVQVGETFK VPSSCPIVTV
360 370 380 390 400
DGYVDPSGGD RFCLGQLSNV HRTEAIERAR LHIGKGVQLE CKGEGDVWVR
410 420 430 440 450
CLSDHAVFVQ SYYLDREAGR APGDAVHKIY PSAYIKVFDL RQCHRQMQQQ
460 470 480 490 500
AATAQAAAAA QAAAVAGNIP GPGSVGGIAP AISLSAAAGI GVDDLRRLCI
510 520 530 540 550
LRMSFVKGWG PDYPRQSIKE TPCWIEIHLH RALQLLDEVL HTMPIADPQP
552
LD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1268 Collapse assay details

Data source: Panorama

Official Gene Symbol
SMAD4
Peptide Sequence
GFPHVIYAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
89
Peptide End
97
CPTAC ID
CPTAC-1268
Peptide Molecular Mass
1,058.5662
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR
CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR
CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK
CPTAC-1223:
MGEA5.
EIPVESIEEVSK
CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK
CPTAC-1226:
VAV1.
LNPGDIVELTK
CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK
CPTAC-1229:
ADCY9.
TDAHFVDVIK
CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK
CPTAC-1232:
B2M.
VEHSDLSFSK
CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK
CPTAC-1235:
CALCOCO1.
LQLEGQVTELR
CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR
CPTAC-1238:
CDKN1B.
VSNGSPSLER
CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR
CPTAC-1241:
ESR1.
LLFAPNLLLDR
CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR
CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR
CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR
CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR
CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1250:
IL18.
SDIIFFQR
CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK
CPTAC-1253:
CDC25B.
LLGHSPVLR
CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER
CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK
CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK
CPTAC-1259:
NEK2.
YSDELNEIITR
CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK
CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR
CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK
CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR
CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK
CPTAC-1268:
SMAD4.
GFPHVIYAR
CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK
CPTAC-1271:
STAT5A.
HILYNEQR
CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR
CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR
CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK
CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 38.5 4.7 10 41.5 8.8 9 56.6 10 13.5 15 15 15
y5 (1+) 18.7 8.7 5.2 24.9 9.7 7.4 31.1 13 9 15 15 15
y7 (1+) 23 7.5 9.2 25.9 8.9 10.7 34.6 11.6 14.1 15 15 15
sum 19 6 4.2 21.7 8.1 5.8 28.8 10.1 7.2 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1269 Collapse assay details

Data source: Panorama

Official Gene Symbol
SMAD4
Peptide Modified Sequence
YC[+57.0]QYAFDLK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
115
Peptide - Site of Modification
2
Peptide Start
114
Peptide End
122
CPTAC ID
CPTAC-1269
Peptide Molecular Mass
1,206.5379
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR
CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR
CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK
CPTAC-1223:
MGEA5.
EIPVESIEEVSK
CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK
CPTAC-1226:
VAV1.
LNPGDIVELTK
CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK
CPTAC-1229:
ADCY9.
TDAHFVDVIK
CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK
CPTAC-1232:
B2M.
VEHSDLSFSK
CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK
CPTAC-1235:
CALCOCO1.
LQLEGQVTELR
CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR
CPTAC-1238:
CDKN1B.
VSNGSPSLER
CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR
CPTAC-1241:
ESR1.
LLFAPNLLLDR
CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR
CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR
CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR
CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR
CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1250:
IL18.
SDIIFFQR
CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK
CPTAC-1253:
CDC25B.
LLGHSPVLR
CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER
CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK
CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK
CPTAC-1259:
NEK2.
YSDELNEIITR
CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK
CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR
CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK
CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR
CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK
CPTAC-1268:
SMAD4.
GFPHVIYAR
CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK
CPTAC-1271:
STAT5A.
HILYNEQR
CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR
CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR
CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK
CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 6.5 5.8 9.2 9.6 10.5 9.8 11.6 12 13.4 15 15 15
y4 (1+) 4.5 2.6 4 11.8 8.3 9.5 12.6 8.7 10.3 15 15 15
y5 (1+) 3.9 2 6.3 10.3 7.6 9.7 11 7.9 11.6 15 15 15
sum 2.9 2.5 5.3 9.7 8.1 9.3 10.1 8.5 10.7 15 15 15

Additional Resources and Comments