GNAI3, CPTAC-1245 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
GNAI3	N/A

Sequence Length (AA)	Molecular Weight (Da)
354	40532

Protein Name
Guanine nucleotide-binding protein G(k) subunit alpha

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGCTLSAEDK	AAVERSKMID	RNLREDGEKA	AKEVKLLLLG	AGESGKSTIV
60	70	80	90	100
KQMKIIHEDG	YSEDECKQYK	VVVYSNTIQS	IIAIIRAMGR	LKIDFGEAAR
110	120	130	140	150
ADDARQLFVL	AGSAEEGVMT	PELAGVIKRL	WRDGGVQACF	SRSREYQLND
160	170	180	190	200
SASYYLNDLD	RISQSNYIPT	QQDVLRTRVK	TTGIVETHFT	FKDLYFKMFD
210	220	230	240	250
VGGQRSERKK	WIHCFEGVTA	IIFCVALSDY	DLVLAEDEEM	NRMHESMKLF
260	270	280	290	300
DSICNNKWFT	ETSIILFLNK	KDLFEEKIKR	SPLTICYPEY	TGSNTYEEAA
310	320	330	340	350
AYIQCQFEDL	NRRKDTKEIY	THFTCATDTK	NVQFVFDAVT	DVIIKNNLKE
354
CGLY

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1245 Collapse assay details

Data source: Panorama

Official Gene Symbol: GNAI3
Peptide Sequence: IDFGEAAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 93
Peptide End: 100

CPTAC ID: CPTAC-1245
Peptide Molecular Mass: 877.4294
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	39.6	9.9	8.5	51.6	11.2	10.7	65	14.9	13.7	15	15	15
y6 (1+)	11.9	8.6	6.5	23.1	10.8	8.3	26	13.8	10.5	15	15	15
y7 (1+)	30	6.3	6	38.4	8.8	7.2	48.7	10.8	9.4	15	15	15
sum	18.6	6.6	6.3	28.6	9.6	8.1	34.1	11.6	10.3	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-1246 Collapse assay details

Data source: Panorama

Official Gene Symbol: GNAI3
Peptide Sequence: ISQSNYIPTQQDVLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 162
Peptide End: 176

CPTAC ID: CPTAC-1246
Peptide Molecular Mass: 1,760.9057
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu