| Official Gene Symbol | Other Aliases |
|---|---|
| IL18 | IGIF, IL1F4 |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 193 | 22326 |
| Protein Name |
|---|
| Interleukin-18 |
| Sources | |
|---|---|
|
UniProt PhosphoSitePlus ® GeneCards |
Human Protein Atlas |
| 10 | 20 | 30 | 40 | 50 |
| MAAEPVEDNC | INFVAMKFID | NTLYFIAEDD | ENLESDYFGK | LESKLSVIRN |
| 60 | 70 | 80 | 90 | 100 |
| LNDQVLFIDQ | GNRPLFEDMT | DSDCRDNAPR | TIFIISMYKD | SQPRGMAVTI |
| 110 | 120 | 130 | 140 | 150 |
| SVKCEKISTL | SCENKIISFK | EMNPPDNIKD | TKSDIIFFQR | SVPGHDNKMQ |
| 160 | 170 | 180 | 190 | 193 |
| FESSSYEGYF | LACEKERDLF | KLILKKEDEL | GDRSIMFTVQ | NED |
Data source: UniProt
Uniprot Database Entry PhosphoSitePlus ®
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Data source: Panorama
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 4.3 | 2.8 | 3.4 | 6.8 | 4.5 | 4.1 | 8 | 5.3 | 5.3 | 15 | 15 | 15 |
| y5 (1+) | 4.7 | 2.5 | 2.3 | 6.7 | 6.3 | 3.5 | 8.2 | 6.8 | 4.2 | 15 | 15 | 15 |
| y4 (1+) | 2.5 | 1.9 | 1.6 | 3.9 | 6.3 | 3.6 | 4.6 | 6.6 | 3.9 | 15 | 15 | 15 |
| sum | 2.2 | 1.8 | 1.7 | 4.3 | 6 | 3.4 | 4.8 | 6.3 | 3.8 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (1+) | 4.5 | 3.8 | 4.2 | 14.5 | 7.5 | 5.9 | 15.2 | 8.4 | 7.2 | 15 | 15 | 15 |
| y6 (1+) | 5.5 | 5.8 | 4 | 15.2 | 9.9 | 4.8 | 16.2 | 11.5 | 6.2 | 15 | 15 | 15 |
| y5 (1+) | 5.6 | 1.7 | 3.3 | 18.2 | 7 | 4 | 19 | 7.2 | 5.2 | 15 | 15 | 15 |
| sum | 3.5 | 2.9 | 3.4 | 15.6 | 7.6 | 4.4 | 16 | 8.1 | 5.6 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 3.9 | 2.8 | 2.9 | 5.6 | 5.2 | 4 | 6.8 | 5.9 | 4.9 | 15 | 15 | 15 |
| y5 (1+) | 2.8 | 1.6 | 2.3 | 4.6 | 5.3 | 3.8 | 5.4 | 5.5 | 4.4 | 15 | 15 | 15 |
| y7 (1+) | 4.1 | 2.1 | 2.2 | 4.8 | 5.5 | 3.4 | 6.3 | 5.9 | 4 | 15 | 15 | 15 |
| sum | 2.6 | 1.4 | 2 | 4.2 | 5.2 | 3.4 | 4.9 | 5.4 | 3.9 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 3 | 0.9 | 2.4 | 5.9 | 4.9 | 3.3 | 6.6 | 5 | 4.1 | 15 | 15 | 15 |
| y4 (1+) | 2.2 | 1 | 2.6 | 7.1 | 5.3 | 2.8 | 7.4 | 5.4 | 3.8 | 15 | 15 | 15 |
| y6 (1+) | 4.3 | 2.1 | 2 | 5.2 | 5.9 | 3.8 | 6.7 | 6.3 | 4.3 | 15 | 15 | 15 |
| sum | 2.4 | 0.8 | 2.4 | 6.5 | 5.2 | 2.8 | 6.9 | 5.3 | 3.7 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 4.8 | 2.3 | 3.3 | 11.2 | 6.4 | 3.2 | 12.2 | 6.8 | 4.6 | 15 | 15 | 15 |
| y7 (1+) | 3.3 | 3.9 | 1.9 | 11.6 | 6.5 | 2.9 | 12.1 | 7.6 | 3.5 | 15 | 15 | 15 |
| y5 (1+) | 5.5 | 2.6 | 2.7 | 12.5 | 8 | 2.2 | 13.7 | 8.4 | 3.5 | 15 | 15 | 15 |
| sum | 3.3 | 2.4 | 1.5 | 11.5 | 6.6 | 1.7 | 12 | 7 | 2.3 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 22.8 | 4.2 | 2.1 | 27.2 | 7.3 | 3.1 | 35.5 | 8.4 | 3.7 | 15 | 15 | 15 |
| y7 (1+) | 14.7 | 4.5 | 1.9 | 26.4 | 6.4 | 3.4 | 30.2 | 7.8 | 3.9 | 15 | 15 | 15 |
| y8 (1+) | 17.7 | 6.3 | 2.2 | 23.8 | 7.1 | 3.7 | 29.7 | 9.5 | 4.3 | 15 | 15 | 15 |
| sum | 14.3 | 3.6 | 1.5 | 21.4 | 5.1 | 3.1 | 25.7 | 6.2 | 3.4 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 6.9 | 3.4 | 1.9 | 8.6 | 12.1 | 13.1 | 11 | 12.6 | 13.2 | 13 | 25 | 14 |
| y7 (1+) | 5.9 | 2.7 | 1.8 | 9.2 | 10.8 | 13 | 10.9 | 11.1 | 13.1 | 13 | 25 | 14 |
| y5 (1+) | 6.9 | 2.8 | 2.8 | 8 | 10.6 | 13.4 | 10.6 | 11 | 13.7 | 13 | 25 | 14 |
| sum | 6.4 | 2.8 | 2.1 | 8.6 | 11 | 13.1 | 10.7 | 11.4 | 13.3 | 13 | 25 | 14 |
Data source: Panorama
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 11.7 | 8.7 | 6.9 | 13.5 | 12 | 8.6 | 17.9 | 14.8 | 11 | 15 | 15 | 15 |
| y6 (1+) | 12.5 | 10 | 6.8 | 17.3 | 10.8 | 9.1 | 21.3 | 14.7 | 11.4 | 15 | 15 | 15 |
| y8 (1+) | 10.3 | 6.9 | 7.4 | 16.2 | 10.4 | 7.3 | 19.2 | 12.5 | 10.4 | 15 | 15 | 15 |
| sum | 6 | 6.8 | 5.1 | 11.8 | 10.5 | 7.6 | 13.2 | 12.5 | 9.2 | 15 | 15 | 15 |
Data source: Panorama
| Slope of Curve Fit for cell line | ||||||
|---|---|---|---|---|---|---|
| fragment_ion | 1 | 2 | 3 | 4 | 5 | 6 |
| y8 (1+) | 1.6 | 1.71 | 1.76 | 1.69 | 1.64 | 1.61 |
| y6 (1+) | 1.66 | 1.74 | 1.87 | 1.69 | 1.62 | 1.85 |
| y5 (1+) | 1.64 | 1.78 | 1.76 | 1.84 | 1.79 | 1.83 |
| sum | 1.63 | 1.75 | 1.78 | 1.76 | 1.7 | 1.76 |
Data source: Panorama
| Fragment ion / Transition | control_intra_CV | actual_temp_intra_CV | frozen_intra_CV | FTx1_intra_CV | FTx2_intra_CV |
|---|---|---|---|---|---|
| y8 (1+) | 7.7 | 7.4 | 5.4 | 1.1 | 4 |
| y6 (1+) | 6.4 | 8.4 | 11.9 | 4.6 | 2.8 |
| y5 (1+) | 6.1 | 6.3 | 34.5 | 1.3 | 4.1 |
| sum | 3.1 | 6.1 | 15.3 | 1 | 3.4 |
| Fragment ion / Transition | all_intra_CV | all_inter_CV |
|---|---|---|
| y8 (1+) | 5.5 | 7.3 |
| y6 (1+) | 7.1 | 9.2 |
| y5 (1+) | 9.8 | 11.7 |
| sum | 5.8 | 7.9 |
Data source: Panorama
| Fragment ion / Transition | intra_CV | inter_CV | total_CV |
|---|---|---|---|
| y6 (1+) | 34.9 | 57.8 | 67.5 |
| y5 (1+) | 29.2 | 50.7 | 58.5 |
| y8 (1+) | 38.5 | 46.4 | 60.3 |
| sum | 24.9 | 45.8 | 52.1 |
Data source: Panorama
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 19.4 | 4.9 | 7.4 | 20.6 | 9.9 | 10.9 | 28.3 | 11 | 13.2 | 15 | 15 | 15 |
| y5 (1+) | 14.3 | 3.5 | 4.2 | 13.6 | 8.6 | 8.8 | 19.7 | 9.3 | 9.8 | 15 | 15 | 15 |
| y6 (1+) | 34.9 | 6.2 | 8.1 | 31.5 | 10.1 | 10.2 | 47 | 11.9 | 13 | 15 | 15 | 15 |
| sum | 13.6 | 3.5 | 5.7 | 15.6 | 8.7 | 9.4 | 20.7 | 9.4 | 11 | 15 | 15 | 15 |
Data source: Panorama
| Slope of Curve Fit for cell line | ||||||
|---|---|---|---|---|---|---|
| fragment_ion | 1 | 2 | 3 | 4 | 5 | 6 |
| y6 (1+) | 1.39 | 1.45 | 1.35 | 1.38 | 1.46 | 1.54 |
| y5 (1+) | 1.43 | 1.47 | 1.39 | 1.38 | 1.63 | 1.6 |
| y4 (1+) | 1.58 | 1.67 | 1.54 | 1.58 | 1.87 | 1.76 |
| sum | 1.49 | 1.56 | 1.46 | 1.47 | 1.72 | 1.67 |
Data source: Panorama
| Fragment ion / Transition | control_intra_CV | actual_temp_intra_CV | frozen_intra_CV | FTx1_intra_CV | FTx2_intra_CV |
|---|---|---|---|---|---|
| Fragment ion / Transition | all_intra_CV | all_inter_CV |
|---|---|---|
Data source: Panorama
| Fragment ion / Transition | intra_CV | inter_CV | total_CV |
|---|---|---|---|
| y6 (1+) | 24.9 | 39.5 | 46.7 |
| y5 (1+) | 21.6 | 37.5 | 43.3 |
| y4 (1+) | 27.8 | 43.4 | 51.5 |
| sum | 23.9 | 40 | 46.6 |
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