| Official Gene Symbol | Other Aliases |
|---|---|
| STAT3 | APRF |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 770 | 88068 |
| Protein Name |
|---|
| Signal transducer and activator of transcription 3 |
| Sources | |
|---|---|
|
UniProt PhosphoSitePlus ® GeneCards |
Human Protein Atlas |
| 10 | 20 | 30 | 40 | 50 |
| MAQWNQLQQL | DTRYLEQLHQ | LYSDSFPMEL | RQFLAPWIES | QDWAYAASKE |
| 60 | 70 | 80 | 90 | 100 |
| SHATLVFHNL | LGEIDQQYSR | FLQESNVLYQ | HNLRRIKQFL | QSRYLEKPME |
| 110 | 120 | 130 | 140 | 150 |
| IARIVARCLW | EESRLLQTAA | TAAQQGGQAN | HPTAAVVTEK | QQMLEQHLQD |
| 160 | 170 | 180 | 190 | 200 |
| VRKRVQDLEQ | KMKVVENLQD | DFDFNYKTLK | SQGDMQDLNG | NNQSVTRQKM |
| 210 | 220 | 230 | 240 | 250 |
| QQLEQMLTAL | DQMRRSIVSE | LAGLLSAMEY | VQKTLTDEEL | ADWKRRQQIA |
| 260 | 270 | 280 | 290 | 300 |
| CIGGPPNICL | DRLENWITSL | AESQLQTRQQ | IKKLEELQQK | VSYKGDPIVQ |
| 310 | 320 | 330 | 340 | 350 |
| HRPMLEERIV | ELFRNLMKSA | FVVERQPCMP | MHPDRPLVIK | TGVQFTTKVR |
| 360 | 370 | 380 | 390 | 400 |
| LLVKFPELNY | QLKIKVCIDK | DSGDVAALRG | SRKFNILGTN | TKVMNMEESN |
| 410 | 420 | 430 | 440 | 450 |
| NGSLSAEFKH | LTLREQRCGN | GGRANCDASL | IVTEELHLIT | FETEVYHQGL |
| 460 | 470 | 480 | 490 | 500 |
| KIDLETHSLP | VVVISNICQM | PNAWASILWY | NMLTNNPKNV | NFFTKPPIGT |
| 510 | 520 | 530 | 540 | 550 |
| WDQVAEVLSW | QFSSTTKRGL | SIEQLTTLAE | KLLGPGVNYS | GCQITWAKFC |
| 560 | 570 | 580 | 590 | 600 |
| KENMAGKGFS | FWVWLDNIID | LVKKYILALW | NEGYIMGFIS | KERERAILST |
| 610 | 620 | 630 | 640 | 650 |
| KPPGTFLLRF | SESSKEGGVT | FTWVEKDISG | KTQIQSVEPY | TKQQLNNMSF |
| 660 | 670 | 680 | 690 | 700 |
| AEIIMGYKIM | DATNILVSPL | VYLYPDIPKE | EAFGKYCRPE | SQEHPEADPG |
| 710 | 720 | 730 | 740 | 750 |
| SAAPYLKTKF | ICVTPTTCSN | TIDLPMSPRT | LDSLMQFGNN | GEGAEPSAGG |
| 760 | 770 | |||
| QFESLTFDME | LTSECATSPM |
Data source: UniProt
Uniprot Database Entry PhosphoSitePlus ®
loading
Data source: Panorama
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 5.6 | 6.8 | 10.5 | 9.5 | 6.7 | 12.7 | 11 | 9.5 | 16.5 | 15 | 15 | 15 |
| y13 (2+) | 15.6 | 6.7 | 10.8 | 16.2 | 5.6 | 14.3 | 22.5 | 8.7 | 17.9 | 15 | 15 | 15 |
| b9 (2+) | 10.1 | 7.1 | 10.8 | 13.6 | 6.2 | 12.8 | 16.9 | 9.4 | 16.7 | 15 | 15 | 15 |
| sum | 5.7 | 6.7 | 10.5 | 8.1 | 6.2 | 12.9 | 9.9 | 9.1 | 16.6 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 15.1 | 15.9 | 8.1 | 21.9 | 21.2 | 12.5 | 26.6 | 26.5 | 14.9 | 13 | 25 | 14 |
| b6 (1+) | 26.9 | 23.1 | 18.1 | 33.2 | 23.5 | 20.4 | 42.7 | 33 | 27.3 | 13 | 25 | 14 |
| b5 (1+) | 16.3 | 17.6 | 13.7 | 18.8 | 19.2 | 14.3 | 24.9 | 26 | 19.8 | 13 | 25 | 14 |
| b4 (1+) | 13.5 | 17.3 | 8.4 | 15.5 | 20.9 | 13.3 | 20.6 | 27.1 | 15.7 | 13 | 25 | 14 |
| b3 (1+) | 17.3 | 5.6 | 6 | 14.5 | 14.1 | 10.8 | 22.6 | 15.2 | 12.4 | 13 | 25 | 14 |
| y4 (1+) | 5.3 | 5.5 | 4.7 | 7.4 | 11.5 | 7.6 | 9.1 | 12.7 | 8.9 | 13 | 25 | 14 |
| y5 (1+) | 10.3 | 6.7 | 5.6 | 11.8 | 13.9 | 11.7 | 15.7 | 15.4 | 13 | 13 | 25 | 14 |
| y6 (1+) | 8.9 | 7.8 | 5.2 | 7.9 | 16 | 12 | 11.9 | 17.8 | 13.1 | 13 | 25 | 14 |
| sum | 8.1 | 3.8 | 4.2 | 10 | 13.2 | 10 | 12.9 | 13.7 | 10.8 | 13 | 25 | 14 |
Data source: Panorama
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b3 (1+) | 9.2 | 4.2 | 3.6 | 10.7 | 4.4 | 4.4 | 14.1 | 6.1 | 5.7 | 15 | 15 | 15 |
| y5 (1+) | 7 | 2.7 | 3.1 | 6.4 | 4.2 | 4.3 | 9.5 | 5 | 5.3 | 15 | 15 | 15 |
| y6 (1+) | 7.9 | 4.8 | 4.1 | 8.1 | 4.7 | 5.9 | 11.3 | 6.7 | 7.2 | 15 | 15 | 15 |
| sum | 3.8 | 2.1 | 2.9 | 4.2 | 3.3 | 4.3 | 5.7 | 3.9 | 5.2 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6-98 (1+) | 28.2 | 9.8 | 7.6 | 38.8 | 13.2 | 5.9 | 48 | 16.4 | 9.6 | 15 | 15 | 15 |
| y5 (1+) | 8.3 | 7.1 | 5.3 | 10.4 | 13.8 | 7.9 | 13.3 | 15.5 | 9.5 | 15 | 15 | 15 |
| y5-98 (1+) | 12.6 | 7.4 | 7.3 | 19.8 | 11.9 | 7.4 | 23.5 | 14 | 10.4 | 15 | 15 | 15 |
| b3 (1+) | 20.1 | 4.7 | 5.2 | 22.8 | 9.3 | 7.1 | 30.4 | 10.4 | 8.8 | 15 | 15 | 15 |
| sum | 9 | 4.5 | 4.8 | 11.6 | 10.2 | 5.8 | 14.7 | 11.1 | 7.5 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (2+) | 12.4 | 5 | 4 | 10.6 | 4.8 | 6 | 16.3 | 6.9 | 7.2 | 15 | 15 | 15 |
| y4 (1+) | 6.4 | 2.1 | 2 | 7.3 | 2.9 | 3.8 | 9.7 | 3.6 | 4.3 | 15 | 15 | 15 |
| b13 (2+) | 4.8 | 1.9 | 3.1 | 6.7 | 2.3 | 3.1 | 8.2 | 3 | 4.4 | 15 | 15 | 15 |
| b9 (1+) | 14.1 | 6.5 | 3.3 | 13.3 | 6.8 | 5.2 | 19.4 | 9.4 | 6.2 | 15 | 15 | 15 |
| b8 (1+) | 13.9 | 2.4 | 4.9 | 11.1 | 4.2 | 4.5 | 17.8 | 4.8 | 6.7 | 15 | 15 | 15 |
| sum | 3.9 | 1.3 | 2 | 5.1 | 1.7 | 3.1 | 6.4 | 2.1 | 3.7 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 2.2 | 2 | 1.2 | 2.7 | 1.4 | 1.6 | 3.5 | 2.4 | 2 | 15 | 15 | 15 |
| b9 (1+) | 5.5 | 3.2 | 2.3 | 4.5 | 3.3 | 3.1 | 7.1 | 4.6 | 3.9 | 15 | 15 | 15 |
| b13 (2+) | 2.6 | 3.2 | 0.9 | 4 | 3.5 | 3 | 4.8 | 4.7 | 3.1 | 15 | 15 | 15 |
| b9 (2+) | 5.3 | 2.9 | 2.4 | 7 | 3.5 | 2.4 | 8.8 | 4.5 | 3.4 | 15 | 15 | 15 |
| sum | 1.5 | 1.3 | 1 | 2.7 | 1.5 | 2.1 | 3.1 | 2 | 2.3 | 15 | 15 | 15 |
Data source: Panorama
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 20.6 | 9.3 | 7.6 | 19.6 | 10.8 | 9 | 28.4 | 14.3 | 11.8 | 15 | 15 | 15 |
| y6 (1+) | 19.1 | 6.7 | 6.1 | 18.3 | 9.9 | 9.1 | 26.5 | 12 | 11 | 15 | 15 | 15 |
| y7 (1+) | 15.6 | 5.3 | 6.9 | 18.7 | 8 | 10.6 | 24.4 | 9.6 | 12.6 | 15 | 15 | 15 |
| sum | 11.9 | 5.8 | 5.6 | 11.7 | 8.5 | 8.8 | 16.7 | 10.3 | 10.4 | 15 | 15 | 15 |
Data source: Panorama
| Slope of Curve Fit for cell line | ||||||
|---|---|---|---|---|---|---|
| fragment_ion | 1 | 2 | 3 | 4 | 5 | 6 |
| y7 (1+) | 0.81 | 0.92 | 0.79 | 0.78 | 0.91 | 0.87 |
| y5 (1+) | 0.8 | 0.84 | 0.91 | 0.76 | 0.9 | 0.84 |
| y6 (1+) | 0.84 | 0.84 | 0.89 | 0.74 | 0.92 | 0.87 |
| sum | 0.82 | 0.87 | 0.86 | 0.76 | 0.91 | 0.87 |
Data source: Panorama
| Fragment ion / Transition | control_intra_CV | actual_temp_intra_CV | frozen_intra_CV | FTx1_intra_CV | FTx2_intra_CV |
|---|---|---|---|---|---|
| y5 (1+) | 10.1 | 3.4 | 0.7 | 7.6 | 1.9 |
| y7 (1+) | 5.3 | 2.4 | 4.7 | 2.3 | 2.4 |
| y6 (1+) | 6.5 | 5.7 | 3 | 4.7 | 2 |
| sum | 6.4 | 2.2 | 3.2 | 2.7 | 0.5 |
| Fragment ion / Transition | all_intra_CV | all_inter_CV |
|---|---|---|
| y5 (1+) | 4.5 | 4.9 |
| y7 (1+) | 3.2 | 4.3 |
| y6 (1+) | 4.6 | 4.5 |
| sum | 2.8 | 2.9 |
Data source: Panorama
| Fragment ion / Transition | intra_CV | inter_CV | total_CV |
|---|---|---|---|
| y6 (1+) | 43.3 | 53.3 | 68.7 |
| y7 (1+) | 41.5 | 58.9 | 72.1 |
| y5 (1+) | 40.9 | 58.4 | 71.3 |
| sum | 30.7 | 48.8 | 57.7 |
Data source: Panorama
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (2+) | 70.1 | 33.3 | 51.6 | 105.1 | 39.9 | 72.1 | 126.3 | 52 | 88.7 | 15 | 15 | 15 |
| y9 (2+) | 48.3 | 22.7 | 17.4 | 63.3 | 27.5 | 27 | 79.6 | 35.7 | 32.1 | 15 | 15 | 15 |
| y9 (1+) | 6.2 | 10 | 13.1 | 27.9 | 16.6 | 16.3 | 28.6 | 19.4 | 20.9 | 15 | 15 | 15 |
| sum | 7.1 | 10 | 13 | 25.9 | 16.1 | 16.3 | 26.9 | 19 | 20.8 | 15 | 15 | 15 |
Data source: Panorama
| Slope of Curve Fit for cell line | ||||||
|---|---|---|---|---|---|---|
| fragment_ion | 1 | 2 | 3 | 4 | 5 | 6 |
| y8 (2+) | 1.94 | 4.74 | 2.91 | 0.35 | 1.69 | 1.2 |
| y9 (1+) | 6.37 | 7.42 | 4.45 | 5.47 | 11.67 | 5.1 |
| y9 (2+) | 4.6 | 8.92 | 4.78 | 4.32 | 8.29 | 4.49 |
| sum | 6.03 | 7.41 | 4.42 | 5.33 | 10.58 | 4.9 |
Data source: Panorama
| Fragment ion / Transition | control_intra_CV | actual_temp_intra_CV | frozen_intra_CV | FTx1_intra_CV | FTx2_intra_CV |
|---|---|---|---|---|---|
| Fragment ion / Transition | all_intra_CV | all_inter_CV |
|---|---|---|
Data source: Panorama
| Fragment ion / Transition | intra_CV | inter_CV | total_CV |
|---|---|---|---|
| y9 (1+) | 50.5 | 61.5 | 79.6 |
| y8 (2+) | 88.6 | 92.1 | 127.8 |
| y9 (2+) | 58.6 | 66.7 | 88.8 |
| sum | 51.6 | 62.4 | 81 |
Data source: Panorama
Data source: Panorama
Pacific Northwest National Laboratory-DirectMRM2
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b13 (2+) | 24.7 | 9.1 | 10.2 | 26.1 | 13.1 | 12.3 | 35.9 | 16 | 16 | 15 | 15 | 15 |
| b8 (1+) | 11.7 | 11.7 | 11.8 | 11.3 | 7.8 | 13.1 | 16.3 | 14.1 | 17.6 | 15 | 15 | 15 |
| b9 (1+) | 15.6 | 7.7 | 10.6 | 16.3 | 10 | 9.8 | 22.6 | 12.6 | 14.4 | 15 | 15 | 15 |
| sum | 12.4 | 4.9 | 6.1 | 11.1 | 7.8 | 6.7 | 16.6 | 9.2 | 9.1 | 15 | 15 | 15 |
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