Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

C3

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
C3 CPAMD1
Sequence Length (AA) Molecular Weight (Da)
1663 187148
Protein Name
Complement C3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGPTSGPSLL LLLLTHLPLA LGSPMYSIIT PNILRLESEE TMVLEAHDAQ
60 70 80 90 100
GDVPVTVTVH DFPGKKLVLS SEKTVLTPAT NHMGNVTFTI PANREFKSEK
110 120 130 140 150
GRNKFVTVQA TFGTQVVEKV VLVSLQSGYL FIQTDKTIYT PGSTVLYRIF
160 170 180 190 200
TVNHKLLPVG RTVMVNIENP EGIPVKQDSL SSQNQLGVLP LSWDIPELVN
210 220 230 240 250
MGQWKIRAYY ENSPQQVFST EFEVKEYVLP SFEVIVEPTE KFYYIYNEKG
260 270 280 290 300
LEVTITARFL YGKKVEGTAF VIFGIQDGEQ RISLPESLKR IPIEDGSGEV
310 320 330 340 350
VLSRKVLLDG VQNPRAEDLV GKSLYVSATV ILHSGSDMVQ AERSGIPIVT
360 370 380 390 400
SPYQIHFTKT PKYFKPGMPF DLMVFVTNPD GSPAYRVPVA VQGEDTVQSL
410 420 430 440 450
TQGDGVAKLS INTHPSQKPL SITVRTKKQE LSEAEQATRT MQALPYSTVG
460 470 480 490 500
NSNNYLHLSV LRTELRPGET LNVNFLLRMD RAHEAKIRYY TYLIMNKGRL
510 520 530 540 550
LKAGRQVREP GQDLVVLPLS ITTDFIPSFR LVAYYTLIGA SGQREVVADS
560 570 580 590 600
VWVDVKDSCV GSLVVKSGQS EDRQPVPGQQ MTLKIEGDHG ARVVLVAVDK
610 620 630 640 650
GVFVLNKKNK LTQSKIWDVV EKADIGCTPG SGKDYAGVFS DAGLTFTSSS
660 670 680 690 700
GQQTAQRAEL QCPQPAARRR RSVQLTEKRM DKVGKYPKEL RKCCEDGMRE
710 720 730 740 750
NPMRFSCQRR TRFISLGEAC KKVFLDCCNY ITELRRQHAR ASHLGLARSN
760 770 780 790 800
LDEDIIAEEN IVSRSEFPES WLWNVEDLKE PPKNGISTKL MNIFLKDSIT
810 820 830 840 850
TWEILAVSMS DKKGICVADP FEVTVMQDFF IDLRLPYSVV RNEQVEIRAV
860 870 880 890 900
LYNYRQNQEL KVRVELLHNP AFCSLATTKR RHQQTVTIPP KSSLSVPYVI
910 920 930 940 950
VPLKTGLQEV EVKAAVYHHF ISDGVRKSLK VVPEGIRMNK TVAVRTLDPE
960 970 980 990 1000
RLGREGVQKE DIPPADLSDQ VPDTESETRI LLQGTPVAQM TEDAVDAERL
1010 1020 1030 1040 1050
KHLIVTPSGC GEQNMIGMTP TVIAVHYLDE TEQWEKFGLE KRQGALELIK
1060 1070 1080 1090 1100
KGYTQQLAFR QPSSAFAAFV KRAPSTWLTA YVVKVFSLAV NLIAIDSQVL
1110 1120 1130 1140 1150
CGAVKWLILE KQKPDGVFQE DAPVIHQEMI GGLRNNNEKD MALTAFVLIS
1160 1170 1180 1190 1200
LQEAKDICEE QVNSLPGSIT KAGDFLEANY MNLQRSYTVA IAGYALAQMG
1210 1220 1230 1240 1250
RLKGPLLNKF LTTAKDKNRW EDPGKQLYNV EATSYALLAL LQLKDFDFVP
1260 1270 1280 1290 1300
PVVRWLNEQR YYGGGYGSTQ ATFMVFQALA QYQKDAPDHQ ELNLDVSLQL
1310 1320 1330 1340 1350
PSRSSKITHR IHWESASLLR SEETKENEGF TVTAEGKGQG TLSVVTMYHA
1360 1370 1380 1390 1400
KAKDQLTCNK FDLKVTIKPA PETEKRPQDA KNTMILEICT RYRGDQDATM
1410 1420 1430 1440 1450
SILDISMMTG FAPDTDDLKQ LANGVDRYIS KYELDKAFSD RNTLIIYLDK
1460 1470 1480 1490 1500
VSHSEDDCLA FKVHQYFNVE LIQPGAVKVY AYYNLEESCT RFYHPEKEDG
1510 1520 1530 1540 1550
KLNKLCRDEL CRCAEENCFI QKSDDKVTLE ERLDKACEPG VDYVYKTRLV
1560 1570 1580 1590 1600
KVQLSNDFDE YIMAIEQTIK SGSDEVQVGQ QRTFISPIKC REALKLEEKK
1610 1620 1630 1640 1650
HYLMWGLSSD FWGEKPNLSY IIGKDTWVEH WPEEDECQDE ENQKQCQDLG
1660 1663
AFTESMVVFG CPN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-1239 Collapse assay details

Data source: Panorama

Official Gene Symbol
C3
Peptide Sequence
TIYTPGSTVLYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
137
Peptide End
148
CPTAC ID
CPTAC-1239
Peptide Molecular Mass
1,369.7242
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR 		CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR 		CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK 		CPTAC-1223:
MGEA5.
EIPVESIEEVSK 		CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK 		CPTAC-1226:
VAV1.
LNPGDIVELTK 		CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK 		CPTAC-1229:
ADCY9.
TDAHFVDVIK 		CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK 		CPTAC-1232:
B2M.
VEHSDLSFSK 		CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK 		CPTAC-1235:
CALCOCO1.
LQLEGQVTELR 		CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR 		CPTAC-1238:
CDKN1B.
VSNGSPSLER 		CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR 		CPTAC-1241:
ESR1.
LLFAPNLLLDR 		CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR 		CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR 		CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR 		CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR 		CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK 		CPTAC-1250:
IL18.
SDIIFFQR 		CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK 		CPTAC-1253:
CDC25B.
LLGHSPVLR 		CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER 		CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK 		CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK 		CPTAC-1259:
NEK2.
YSDELNEIITR 		CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK 		CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR 		CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK 		CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR 		CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK 		CPTAC-1268:
SMAD4.
GFPHVIYAR 		CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK 		CPTAC-1271:
STAT5A.
HILYNEQR 		CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR 		CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR 		CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK 		CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 8.6 5.6 8.4 15.6 9 8.5 17.8 10.6 12 15 15 15
y9 (1+) 35.1 8.5 10.1 24.5 11.2 10.5 42.8 14.1 14.6 15 15 15
y10 (1+) 23.9 6.2 7.9 23.4 7.4 10.3 33.4 9.7 13 15 15 15
sum 12.9 5.6 8 13.8 8.2 8.8 18.9 9.9 11.9 15 15 15

Selectivity

Data source: Panorama

view larger image
Slope of Curve Fit for cell line
fragment_ion 1 2 3 4 5 6
y9 (1+) 0.36 0.34 0.35 0.36 0.38 0.39
y10 (1+) 0.34 0.34 0.34 0.34 0.34 0.37
y8 (1+) 0.38 0.39 0.39 0.4 0.46 0.42
sum 0.36 0.37 0.37 0.38 0.41 0.4

Stability

Data source: Panorama

view larger image
Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
y10 (1+) 5.1 5.8 6.4 0.9 2.5
y8 (1+) 4.7 1.3 2.6 5.4 5.9
y9 (1+) 5.5 4.6 6.6 9.2 3.8
sum 4 2.1 2.7 4.3 4.4
Fragment ion / Transition all_intra_CV all_inter_CV
y10 (1+) 4.4 4.6
y8 (1+) 3.5 4.5
y9 (1+) 5.7 7
sum 3.3 4.2

Endogenous

Data source: Panorama

view larger image
Fragment ion / Transition intra_CV inter_CV total_CV
y9 (1+) 36 45.4 57.9
y8 (1+) 26.1 43.4 50.6
y10 (1+) 29.8 36.1 46.8
sum 26 40 47.7

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-5596 Collapse assay details

Data source: Panorama

Official Gene Symbol
C3
Peptide Sequence
GTLSVVAVYHAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1340
Peptide End
1351
CPTAC ID
non-CPTAC-5596
Peptide Molecular Mass
1,243.6925
Species
Mus musculus (Mouse)
Assay Type
Direct MRM or SRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR 		CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR 		CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK 		CPTAC-1223:
MGEA5.
EIPVESIEEVSK 		CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK 		CPTAC-1226:
VAV1.
LNPGDIVELTK 		CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK 		CPTAC-1229:
ADCY9.
TDAHFVDVIK 		CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK 		CPTAC-1232:
B2M.
VEHSDLSFSK 		CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK 		CPTAC-1235:
CALCOCO1.
LQLEGQVTELR 		CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR 		CPTAC-1238:
CDKN1B.
VSNGSPSLER 		CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR 		CPTAC-1241:
ESR1.
LLFAPNLLLDR 		CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR 		CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR 		CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR 		CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR 		CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK 		CPTAC-1250:
IL18.
SDIIFFQR 		CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK 		CPTAC-1253:
CDC25B.
LLGHSPVLR 		CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER 		CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK 		CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK 		CPTAC-1259:
NEK2.
YSDELNEIITR 		CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK 		CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR 		CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK 		CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR 		CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK 		CPTAC-1268:
SMAD4.
GFPHVIYAR 		CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK 		CPTAC-1271:
STAT5A.
HILYNEQR 		CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR 		CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR 		CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK 		CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (3+) 19.7 10 9.5 28.6 12.5 10 34.7 16 13.8 15 15 15
y9 (2+) 6.2 3.2 3.9 6.9 5 4.3 9.3 5.9 5.8 15 15 15
y10 (2+) 6.8 5.1 3.8 7.9 7 5.1 10.4 8.7 6.4 15 15 15
y4 (1+) 7.8 6.9 2.7 9.8 6.6 4.2 12.5 9.5 5 15 15 15
y6 (1+) 6.9 3.1 2.2 10.6 6.6 2.9 12.6 7.3 3.6 15 15 15
sum 3.5 2.9 1.6 5.4 5.6 2.7 6.4 6.3 3.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-1106 Collapse assay details

Data source: Panorama

Official Gene Symbol
C3
Peptide Sequence
SGIPIVTSPYQIHFTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
344
Peptide End
359
CPTAC ID
non-CPTAC-1106
Peptide Molecular Mass
1,786.9618
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR 		CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR 		CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK 		CPTAC-1223:
MGEA5.
EIPVESIEEVSK 		CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK 		CPTAC-1226:
VAV1.
LNPGDIVELTK 		CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK 		CPTAC-1229:
ADCY9.
TDAHFVDVIK 		CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK 		CPTAC-1232:
B2M.
VEHSDLSFSK 		CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK 		CPTAC-1235:
CALCOCO1.
LQLEGQVTELR 		CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR 		CPTAC-1238:
CDKN1B.
VSNGSPSLER 		CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR 		CPTAC-1241:
ESR1.
LLFAPNLLLDR 		CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR 		CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR 		CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR 		CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR 		CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK 		CPTAC-1250:
IL18.
SDIIFFQR 		CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK 		CPTAC-1253:
CDC25B.
LLGHSPVLR 		CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER 		CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK 		CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK 		CPTAC-1259:
NEK2.
YSDELNEIITR 		CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK 		CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR 		CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK 		CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR 		CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK 		CPTAC-1268:
SMAD4.
GFPHVIYAR 		CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK 		CPTAC-1271:
STAT5A.
HILYNEQR 		CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR 		CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR 		CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK 		CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b14 (3+) NA NA NA NA NA NA NA NA NA 0 0 0
b3 (1+) 5.5 3 2.7 14.3 7.1 6 15.3 7.7 6.6 15 15 15
y13 (3+) 5.9 3.4 4.3 13.5 7.1 5.5 14.7 7.9 7 15 15 15
y13 (2+) 6 4.5 3.6 13.2 7.4 5.8 14.5 8.7 6.8 15 15 15
sum 5.4 1.7 2.9 13.1 5.9 5 14.2 6.1 5.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-1105 Collapse assay details

Data source: Panorama

Official Gene Symbol
C3
Peptide Sequence
TGLQEVEVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
905
Peptide End
913
CPTAC ID
non-CPTAC-1105
Peptide Molecular Mass
1,001.5393
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR 		CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR 		CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK 		CPTAC-1223:
MGEA5.
EIPVESIEEVSK 		CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK 		CPTAC-1226:
VAV1.
LNPGDIVELTK 		CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK 		CPTAC-1229:
ADCY9.
TDAHFVDVIK 		CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK 		CPTAC-1232:
B2M.
VEHSDLSFSK 		CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK 		CPTAC-1235:
CALCOCO1.
LQLEGQVTELR 		CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR 		CPTAC-1238:
CDKN1B.
VSNGSPSLER 		CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR 		CPTAC-1241:
ESR1.
LLFAPNLLLDR 		CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR 		CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR 		CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR 		CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR 		CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK 		CPTAC-1250:
IL18.
SDIIFFQR 		CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK 		CPTAC-1253:
CDC25B.
LLGHSPVLR 		CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER 		CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK 		CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK 		CPTAC-1259:
NEK2.
YSDELNEIITR 		CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK 		CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR 		CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK 		CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR 		CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK 		CPTAC-1268:
SMAD4.
GFPHVIYAR 		CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK 		CPTAC-1271:
STAT5A.
HILYNEQR 		CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR 		CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR 		CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK 		CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (2+) 6.5 4.8 5 13 6.4 6.6 14.5 8 8.3 15 15 15
y2 (1+) 4.9 4.5 4.5 8.8 6.5 4.2 10.1 7.9 6.2 15 15 15
y5 (1+) 5.7 6.8 3.8 8.4 9.1 7 10.2 11.4 8 15 15 15
y6 (1+) 6.3 4.2 5 11.7 9.5 6.1 13.3 10.4 7.9 15 15 15
sum 2.7 2 2.1 8.9 6.4 3.5 9.3 6.7 4.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-2653 Collapse assay details

Data source: Panorama

Official Gene Symbol
C3
Peptide Sequence
VHQYFNVELIQPGAVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1463
Peptide End
1478
CPTAC ID
non-CPTAC-2653
Peptide Molecular Mass
1,840.9836
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR 		CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR 		CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK 		CPTAC-1223:
MGEA5.
EIPVESIEEVSK 		CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK 		CPTAC-1226:
VAV1.
LNPGDIVELTK 		CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK 		CPTAC-1229:
ADCY9.
TDAHFVDVIK 		CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK 		CPTAC-1232:
B2M.
VEHSDLSFSK 		CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK 		CPTAC-1235:
CALCOCO1.
LQLEGQVTELR 		CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR 		CPTAC-1238:
CDKN1B.
VSNGSPSLER 		CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR 		CPTAC-1241:
ESR1.
LLFAPNLLLDR 		CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR 		CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR 		CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR 		CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR 		CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK 		CPTAC-1250:
IL18.
SDIIFFQR 		CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK 		CPTAC-1253:
CDC25B.
LLGHSPVLR 		CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER 		CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK 		CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK 		CPTAC-1259:
NEK2.
YSDELNEIITR 		CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK 		CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR 		CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK 		CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR 		CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK 		CPTAC-1268:
SMAD4.
GFPHVIYAR 		CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK 		CPTAC-1271:
STAT5A.
HILYNEQR 		CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR 		CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR 		CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK 		CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 9.7 4.9 3.5 17 9 6.6 19.6 10.2 7.5 15 15 15
b6 (1+) 8.5 7.7 4.9 13 7.2 8.7 15.5 10.5 10 15 15 15
y6 (1+) 6.2 4.8 5.4 17 9.8 5.7 18.1 10.9 7.9 15 15 15
y7 (1+) 9.4 6.9 3.6 17 8.2 5.3 19.4 10.7 6.4 15 15 15
b13 (3+) 11.9 5.9 6.3 17.4 7.2 7.1 21.1 9.3 9.5 15 15 15
sum 5.8 3.2 2.6 13.2 6.1 4.2 14.4 6.9 4.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-5595 Collapse assay details

Data source: Panorama

Official Gene Symbol
C3
Peptide Sequence
VHQYFNVGLIQPGSVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1463
Peptide End
1478
CPTAC ID
non-CPTAC-5595
Peptide Molecular Mass
1,784.9574
Species
Mus musculus (Mouse)
Assay Type
Direct MRM or SRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR 		CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR 		CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK 		CPTAC-1223:
MGEA5.
EIPVESIEEVSK 		CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK 		CPTAC-1226:
VAV1.
LNPGDIVELTK 		CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK 		CPTAC-1229:
ADCY9.
TDAHFVDVIK 		CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK 		CPTAC-1232:
B2M.
VEHSDLSFSK 		CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK 		CPTAC-1235:
CALCOCO1.
LQLEGQVTELR 		CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR 		CPTAC-1238:
CDKN1B.
VSNGSPSLER 		CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR 		CPTAC-1241:
ESR1.
LLFAPNLLLDR 		CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR 		CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR 		CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR 		CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR 		CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK 		CPTAC-1250:
IL18.
SDIIFFQR 		CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK 		CPTAC-1253:
CDC25B.
LLGHSPVLR 		CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER 		CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK 		CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK 		CPTAC-1259:
NEK2.
YSDELNEIITR 		CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK 		CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR 		CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK 		CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR 		CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK 		CPTAC-1268:
SMAD4.
GFPHVIYAR 		CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK 		CPTAC-1271:
STAT5A.
HILYNEQR 		CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR 		CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR 		CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK 		CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b9 (2+) 5.1 2.7 2.9 7 6.7 3.4 8.7 7.2 4.5 15 15 15
b6 (1+) 4.6 3.8 5 9 6.8 4 10.1 7.8 6.4 15 15 15
b2 (1+) 4.6 2.7 2.8 6.6 6.4 3.6 8 6.9 4.6 15 15 15
y5 (1+) 4.6 1.5 4.2 6.2 5.1 5.8 7.7 5.3 7.2 15 15 15
y7 (1+) 3.5 3.8 3.3 8.4 6.8 2.8 9.1 7.8 4.3 15 15 15
sum 1 1 1.2 5.9 5.4 2.7 6 5.5 3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments