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Overview Data source: UniProt

Official Gene Symbol Other Aliases
C3 N/A
Sequence Length (AA) Molecular Weight (Da)
1663 186484
Protein Name
Complement C3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGPASGSQLL VLLLLLASSP LALGIPMYSI ITPNVLRLES EETIVLEAHD
60 70 80 90 100
AQGDIPVTVT VQDFLKRQVL TSEKTVLTGA SGHLRSVSIK IPASKEFNSD
110 120 130 140 150
KEGHKYVTVV ANFGETVVEK AVMVSFQSGY LFIQTDKTIY TPGSTVLYRI
160 170 180 190 200
FTVDNNLLPV GKTVVILIET PDGIPVKRDI LSSNNQHGIL PLSWNIPELV
210 220 230 240 250
NMGQWKIRAF YEHAPKQIFS AEFEVKEYVL PSFEVRVEPT ETFYYIDDPN
260 270 280 290 300
GLEVSIIAKF LYGKNVDGTA FVIFGVQDGD KKISLAHSLT RVVIEDGVGD
310 320 330 340 350
AVLTRKVLME GVRPSNADAL VGKSLYVSVT VILHSGSDMV EAERSGIPIV
360 370 380 390 400
TSPYQIHFTK TPKFFKPAMP FDLMVFVTNP DGSPASKVLV VTQGSNAKAL
410 420 430 440 450
TQDDGVAKLS INTPNSRQPL TITVRTKKDT LPESRQATKT MEAHPYSTMH
460 470 480 490 500
NSNNYLHLSV SRMELKPGDN LNVNFHLRTD PGHEAKIRYY TYLVMNKGKL
510 520 530 540 550
LKAGRQVREP GQDLVVLSLP ITPEFIPSFR LVAYYTLIGA SGQREVVADS
560 570 580 590 600
VWVDVKDSCI GTLVVKGDPR DNHLAPGQQT TLRIEGNQGA RVGLVAVDKG
610 620 630 640 650
VFVLNKKNKL TQSKIWDVVE KADIGCTPGS GKNYAGVFMD AGLAFKTSQG
660 670 680 690 700
LQTEQRADLE CTKPAARRRR SVQLMERRMD KAGQYTDKGL RKCCEDGMRD
710 720 730 740 750
IPMRYSCQRR ARLITQGENC IKAFIDCCNH ITKLREQHRR DHVLGLARSE
760 770 780 790 800
LEEDIIPEED IISRSHFPQS WLWTIEELKE PEKNGISTKV MNIFLKDSIT
810 820 830 840 850
TWEILAVSLS DKKGICVADP YEIRVMQDFF IDLRLPYSVV RNEQVEIRAV
860 870 880 890 900
LFNYREQEEL KVRVELLHNP AFCSMATAKN RYFQTIKIPP KSSVAVPYVI
910 920 930 940 950
VPLKIGQQEV EVKAAVFNHF ISDGVKKTLK VVPEGMRINK TVAIHTLDPE
960 970 980 990 1000
KLGQGGVQKV DVPAADLSDQ VPDTDSETRI ILQGSPVVQM AEDAVDGERL
1010 1020 1030 1040 1050
KHLIVTPAGC GEQNMIGMTP TVIAVHYLDQ TEQWEKFGIE KRQEALELIK
1060 1070 1080 1090 1100
KGYTQQLAFK QPSSAYAAFN NRPPSTWLTA YVVKVFSLAA NLIAIDSHVL
1110 1120 1130 1140 1150
CGAVKWLILE KQKPDGVFQE DGPVIHQEMI GGFRNAKEAD VSLTAFVLIA
1160 1170 1180 1190 1200
LQEARDICEG QVNSLPGSIN KAGEYIEASY MNLQRPYTVA IAGYALALMN
1210 1220 1230 1240 1250
KLEEPYLGKF LNTAKDRNRW EEPDQQLYNV EATSYALLAL LLLKDFDSVP
1260 1270 1280 1290 1300
PVVRWLNEQR YYGGGYGSTQ ATFMVFQALA QYQTDVPDHK DLNMDVSFHL
1310 1320 1330 1340 1350
PSRSSATTFR LLWENGNLLR SEETKQNEAF SLTAKGKGRG TLSVVAVYHA
1360 1370 1380 1390 1400
KLKSKVTCKK FDLRVSIRPA PETAKKPEEA KNTMFLEICT KYLGDVDATM
1410 1420 1430 1440 1450
SILDISMMTG FAPDTKDLEL LASGVDRYIS KYEMNKAFSN KNTLIIYLEK
1460 1470 1480 1490 1500
ISHTEEDCLT FKVHQYFNVG LIQPGSVKVY SYYNLEESCT RFYHPEKDDG
1510 1520 1530 1540 1550
MLSKLCHSEM CRCAEENCFM QQSQEKINLN VRLDKACEPG VDYVYKTELT
1560 1570 1580 1590 1600
NIELLDDFDE YTMTIQQVIK SGSDEVQAGQ QRKFISHIKC RNALKLQKGK
1610 1620 1630 1640 1650
KYLMWGLSSD LWGEKPNTSY IIGKDTWVEH WPEAEECQDQ KYQKQCEELG
1660 1663
AFTESMVVYG CPN

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1239 Collapse assay details

Data source: Panorama

Official Gene Symbol
C3
Peptide Sequence
TIYTPGSTVLYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
137
Peptide End
148
CPTAC ID
CPTAC-1239
Peptide Molecular Mass
1,369.7242
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 8.6 5.6 8.4 15.6 9 8.5 17.8 10.6 12 15 15 15
y9 (1+) 35.1 8.5 10.1 24.5 11.2 10.5 42.8 14.1 14.6 15 15 15
y10 (1+) 23.9 6.2 7.9 23.4 7.4 10.3 33.4 9.7 13 15 15 15
sum 12.9 5.6 8 13.8 8.2 8.8 18.9 9.9 11.9 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1106 Collapse assay details

Data source: Panorama

Official Gene Symbol
C3
Peptide Sequence
SGIPIVTSPYQIHFTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
344
Peptide End
359
CPTAC ID
non-CPTAC-1106
Peptide Molecular Mass
1,786.9618
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b14 (3+) NA NA NA NA NA NA NA NA NA 0 0 0
b3 (1+) 5.5 3 2.7 14.3 7.1 6 15.3 7.7 6.6 15 15 15
y13 (3+) 5.9 3.4 4.3 13.5 7.1 5.5 14.7 7.9 7 15 15 15
y13 (2+) 6 4.5 3.6 13.2 7.4 5.8 14.5 8.7 6.8 15 15 15
sum 5.4 1.7 2.9 13.1 5.9 5 14.2 6.1 5.8 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1105 Collapse assay details

Data source: Panorama

Official Gene Symbol
C3
Peptide Sequence
TGLQEVEVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
905
Peptide End
913
CPTAC ID
non-CPTAC-1105
Peptide Molecular Mass
1,001.5393
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (2+) 6.5 4.8 5 13 6.4 6.6 14.5 8 8.3 15 15 15
y2 (1+) 4.9 4.5 4.5 8.8 6.5 4.2 10.1 7.9 6.2 15 15 15
y5 (1+) 5.7 6.8 3.8 8.4 9.1 7 10.2 11.4 8 15 15 15
y6 (1+) 6.3 4.2 5 11.7 9.5 6.1 13.3 10.4 7.9 15 15 15
sum 2.7 2 2.1 8.9 6.4 3.5 9.3 6.7 4.1 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-2653 Collapse assay details

Data source: Panorama

Official Gene Symbol
C3
Peptide Sequence
VHQYFNVELIQPGAVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1463
Peptide End
1478
CPTAC ID
non-CPTAC-2653
Peptide Molecular Mass
1,840.9836
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 9.7 4.9 3.5 17 9 6.6 19.6 10.2 7.5 15 15 15
b6 (1+) 8.5 7.7 4.9 13 7.2 8.7 15.5 10.5 10 15 15 15
y6 (1+) 6.2 4.8 5.4 17 9.8 5.7 18.1 10.9 7.9 15 15 15
y7 (1+) 9.4 6.9 3.6 17 8.2 5.3 19.4 10.7 6.4 15 15 15
b13 (3+) 11.9 5.9 6.3 17.4 7.2 7.1 21.1 9.3 9.5 15 15 15
sum 5.8 3.2 2.6 13.2 6.1 4.2 14.4 6.9 4.9 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-5595 Collapse assay details

Data source: Panorama

Official Gene Symbol
C3
Peptide Sequence
VHQYFNVGLIQPGSVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1463
Peptide End
1478
CPTAC ID
non-CPTAC-5595
Peptide Molecular Mass
1,784.9574
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b9 (2+) 5.1 2.7 2.9 7 6.7 3.4 8.7 7.2 4.5 15 15 15
b6 (1+) 4.6 3.8 5 9 6.8 4 10.1 7.8 6.4 15 15 15
b2 (1+) 4.6 2.7 2.8 6.6 6.4 3.6 8 6.9 4.6 15 15 15
y5 (1+) 4.6 1.5 4.2 6.2 5.1 5.8 7.7 5.3 7.2 15 15 15
y7 (1+) 3.5 3.8 3.3 8.4 6.8 2.8 9.1 7.8 4.3 15 15 15
sum 1 1 1.2 5.9 5.4 2.7 6 5.5 3 15 15 15

Additional Resources and Comments