C3, CPTAC-5595 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
C3	N/A

Sequence Length (AA)	Molecular Weight (Da)
1663	186484

Protein Name
Complement C3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGPASGSQLL	VLLLLLASSP	LALGIPMYSI	ITPNVLRLES	EETIVLEAHD
60	70	80	90	100
AQGDIPVTVT	VQDFLKRQVL	TSEKTVLTGA	SGHLRSVSIK	IPASKEFNSD
110	120	130	140	150
KEGHKYVTVV	ANFGETVVEK	AVMVSFQSGY	LFIQTDKTIY	TPGSTVLYRI
160	170	180	190	200
FTVDNNLLPV	GKTVVILIET	PDGIPVKRDI	LSSNNQHGIL	PLSWNIPELV
210	220	230	240	250
NMGQWKIRAF	YEHAPKQIFS	AEFEVKEYVL	PSFEVRVEPT	ETFYYIDDPN
260	270	280	290	300
GLEVSIIAKF	LYGKNVDGTA	FVIFGVQDGD	KKISLAHSLT	RVVIEDGVGD
310	320	330	340	350
AVLTRKVLME	GVRPSNADAL	VGKSLYVSVT	VILHSGSDMV	EAERSGIPIV
360	370	380	390	400
TSPYQIHFTK	TPKFFKPAMP	FDLMVFVTNP	DGSPASKVLV	VTQGSNAKAL
410	420	430	440	450
TQDDGVAKLS	INTPNSRQPL	TITVRTKKDT	LPESRQATKT	MEAHPYSTMH
460	470	480	490	500
NSNNYLHLSV	SRMELKPGDN	LNVNFHLRTD	PGHEAKIRYY	TYLVMNKGKL
510	520	530	540	550
LKAGRQVREP	GQDLVVLSLP	ITPEFIPSFR	LVAYYTLIGA	SGQREVVADS
560	570	580	590	600
VWVDVKDSCI	GTLVVKGDPR	DNHLAPGQQT	TLRIEGNQGA	RVGLVAVDKG
610	620	630	640	650
VFVLNKKNKL	TQSKIWDVVE	KADIGCTPGS	GKNYAGVFMD	AGLAFKTSQG
660	670	680	690	700
LQTEQRADLE	CTKPAARRRR	SVQLMERRMD	KAGQYTDKGL	RKCCEDGMRD
710	720	730	740	750
IPMRYSCQRR	ARLITQGENC	IKAFIDCCNH	ITKLREQHRR	DHVLGLARSE
760	770	780	790	800
LEEDIIPEED	IISRSHFPQS	WLWTIEELKE	PEKNGISTKV	MNIFLKDSIT
810	820	830	840	850
TWEILAVSLS	DKKGICVADP	YEIRVMQDFF	IDLRLPYSVV	RNEQVEIRAV
860	870	880	890	900
LFNYREQEEL	KVRVELLHNP	AFCSMATAKN	RYFQTIKIPP	KSSVAVPYVI
910	920	930	940	950
VPLKIGQQEV	EVKAAVFNHF	ISDGVKKTLK	VVPEGMRINK	TVAIHTLDPE
960	970	980	990	1000
KLGQGGVQKV	DVPAADLSDQ	VPDTDSETRI	ILQGSPVVQM	AEDAVDGERL
1010	1020	1030	1040	1050
KHLIVTPAGC	GEQNMIGMTP	TVIAVHYLDQ	TEQWEKFGIE	KRQEALELIK
1060	1070	1080	1090	1100
KGYTQQLAFK	QPSSAYAAFN	NRPPSTWLTA	YVVKVFSLAA	NLIAIDSHVL
1110	1120	1130	1140	1150
CGAVKWLILE	KQKPDGVFQE	DGPVIHQEMI	GGFRNAKEAD	VSLTAFVLIA
1160	1170	1180	1190	1200
LQEARDICEG	QVNSLPGSIN	KAGEYIEASY	MNLQRPYTVA	IAGYALALMN
1210	1220	1230	1240	1250
KLEEPYLGKF	LNTAKDRNRW	EEPDQQLYNV	EATSYALLAL	LLLKDFDSVP
1260	1270	1280	1290	1300
PVVRWLNEQR	YYGGGYGSTQ	ATFMVFQALA	QYQTDVPDHK	DLNMDVSFHL
1310	1320	1330	1340	1350
PSRSSATTFR	LLWENGNLLR	SEETKQNEAF	SLTAKGKGRG	TLSVVAVYHA
1360	1370	1380	1390	1400
KLKSKVTCKK	FDLRVSIRPA	PETAKKPEEA	KNTMFLEICT	KYLGDVDATM
1410	1420	1430	1440	1450
SILDISMMTG	FAPDTKDLEL	LASGVDRYIS	KYEMNKAFSN	KNTLIIYLEK
1460	1470	1480	1490	1500
ISHTEEDCLT	FKVHQYFNVG	LIQPGSVKVY	SYYNLEESCT	RFYHPEKDDG
1510	1520	1530	1540	1550
MLSKLCHSEM	CRCAEENCFM	QQSQEKINLN	VRLDKACEPG	VDYVYKTELT
1560	1570	1580	1590	1600
NIELLDDFDE	YTMTIQQVIK	SGSDEVQAGQ	QRKFISHIKC	RNALKLQKGK
1610	1620	1630	1640	1650
KYLMWGLSSD	LWGEKPNTSY	IIGKDTWVEH	WPEAEECQDQ	KYQKQCEELG
1660	1663
AFTESMVVYG	CPN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1239 Collapse assay details

Data source: Panorama

Official Gene Symbol: C3
Peptide Sequence: TIYTPGSTVLYR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 137
Peptide End: 148

CPTAC ID: CPTAC-1239
Peptide Molecular Mass: 1,369.7242
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	8.6	5.6	8.4	15.6	9	8.5	17.8	10.6	12	15	15	15
y9 (1+)	35.1	8.5	10.1	24.5	11.2	10.5	42.8	14.1	14.6	15	15	15
y10 (1+)	23.9	6.2	7.9	23.4	7.4	10.3	33.4	9.7	13	15	15	15
sum	12.9	5.6	8	13.8	8.2	8.8	18.9	9.9	11.9	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-5596 Collapse assay details

Data source: Panorama

Official Gene Symbol: C3
Peptide Sequence: GTLSVVAVYHAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 1340
Peptide End: 1351

CPTAC ID: non-CPTAC-5596
Peptide Molecular Mass: 1,243.6925
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.