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Overview Data source: UniProt

Official Gene Symbol Other Aliases
STAT4 N/A
Sequence Length (AA) Molecular Weight (Da)
748 85941
Protein Name
Signal transducer and activator of transcription 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSQWNQVQQL EIKFLEQVDQ FYDDNFPMEI RHLLAQWIEN QDWEAASNNE
60 70 80 90 100
TMATILLQNL LIQLDEQLGR VSKEKNLLLI HNLKRIRKVL QGKFHGNPMH
110 120 130 140 150
VAVVISNCLR EERRILAAAN MPVQGPLEKS LQSSSVSERQ RNVEHKVAAI
160 170 180 190 200
KNSVQMTEQD TKYLEDLQDE FDYRYKTIQT MDQSDKNSAM VNQEVLTLQE
210 220 230 240 250
MLNSLDFKRK EALSKMTQII HETDLLMNTM LIEELQDWKR RQQIACIGGP
260 270 280 290 300
LHNGLDQLQN CFTLLAESLF QLRRQLEKLE EQSTKMTYEG DPIPMQRTHM
310 320 330 340 350
LERVTFLIYN LFKNSFVVER QPCMPTHPQR PLVLKTLIQF TVKLRLLIKL
360 370 380 390 400
PELNYQVKVK ASIDKNVSTL SNRRFVLCGT NVKAMSIEES SNGSLSVEFR
410 420 430 440 450
HLQPKEMKSS AGGKGNEGCH MVTEELHSIT FETQICLYGL TIDLETSSLP
460 470 480 490 500
VVMISNVSQL PNAWASIIWY NVSTNDSQNL VFFNNPPPAT LSQLLEVMSW
510 520 530 540 550
QFSSYVGRGL NSDQLHMLAE KLTVQSSYSD GHLTWAKFCK EHLPGKSFTF
560 570 580 590 600
WTWLEAILDL IKKHILPLWI DGYVMGFVSK EKERLLLKDK MPGTFLLRFS
610 620 630 640 650
ESHLGGITFT WVDHSESGEV RFHSVEPYNK GRLSALPFAD ILRDYKVIMA
660 670 680 690 700
ENIPENPLKY LYPDIPKDKA FGKHYSSQPC EVSRPTERGD KGYVPSVFIP
710 720 730 740 748
ISTIRSDSTE PHSPSDLLPM SPSVYAVLRE NLSPTTIETA MKSPYSAE

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1277 Collapse assay details

Data source: Panorama

Official Gene Symbol
STAT4
Peptide Sequence
YLYPDIPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
660
Peptide End
667
CPTAC ID
CPTAC-1277
Peptide Molecular Mass
1,007.5328
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR
CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR
CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK
CPTAC-1223:
MGEA5.
EIPVESIEEVSK
CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK
CPTAC-1226:
VAV1.
LNPGDIVELTK
CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK
CPTAC-1229:
ADCY9.
TDAHFVDVIK
CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK
CPTAC-1232:
B2M.
VEHSDLSFSK
CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK
CPTAC-1235:
CALCOCO1.
LQLEGQVTELR
CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR
CPTAC-1238:
CDKN1B.
VSNGSPSLER
CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR
CPTAC-1241:
ESR1.
LLFAPNLLLDR
CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR
CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR
CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR
CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR
CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1250:
IL18.
SDIIFFQR
CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK
CPTAC-1253:
CDC25B.
LLGHSPVLR
CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER
CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK
CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK
CPTAC-1259:
NEK2.
YSDELNEIITR
CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK
CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR
CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK
CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR
CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK
CPTAC-1268:
SMAD4.
GFPHVIYAR
CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK
CPTAC-1271:
STAT5A.
HILYNEQR
CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR
CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR
CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK
CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 7.1 6.4 4.8 8.7 8.7 10.2 11.2 10.8 11.3 15 15 15
y6 (1+) 11.6 4.5 4.5 15.9 7 8.4 19.7 8.3 9.5 15 15 15
y7 (1+) 21.2 4.8 8.5 23.6 8.6 10.6 31.7 9.8 13.6 15 15 15
sum 8.2 4.7 4.6 13.2 7.4 8.9 15.5 8.8 10 15 15 15

Additional Resources and Comments