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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

NCOA1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
NCOA1 BHLHE74, SRC1
Sequence Length (AA) Molecular Weight (Da)
1441 156757
Protein Name
Nuclear receptor coactivator 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSGLGDSSSD PANPDSHKRK GSPCDTLASS TEKRRREQEN KYLEELAELL
60 70 80 90 100
SANISDIDSL SVKPDKCKIL KKTVDQIQLM KRMEQEKSTT DDDVQKSDIS
110 120 130 140 150
SSSQGVIEKE SLGPLLLEAL DGFFFVVNCE GRIVFVSENV TSYLGYNQEE
160 170 180 190 200
LMNTSVYSIL HVGDHAEFVK NLLPKSLVNG VPWPQEATRR NSHTFNCRML
210 220 230 240 250
IHPPDEPGTE NQEACQRYEV MQCFTVSQPK SIQEDGEDFQ SCLICIARRL
260 270 280 290 300
PRPPAITGVE SFMTKQDTTG KIISIDTSSL RAAGRTGWED LVRKCIYAFF
310 320 330 340 350
QPQGREPSYA RQLFQEVMTR GTASSPSYRF ILNDGTMLSA HTKCKLCYPQ
360 370 380 390 400
SPDMQPFIMG IHIIDREHSG LSPQDDTNSG MSIPRVNPSV NPSISPAHGV
410 420 430 440 450
ARSSTLPPSN SNMVSTRINR QQSSDLHSSS HSNSSNSQGS FGCSPGSQIV
460 470 480 490 500
ANVALNQGQA SSQSSNPSLN LNNSPMEGTG ISLAQFMSPR RQVTSGLATR
510 520 530 540 550
PRMPNNSFPP NISTLSSPVG MTSSACNNNN RSYSNIPVTS LQGMNEGPNN
560 570 580 590 600
SVGFSASSPV LRQMSSQNSP SRLNIQPAKA ESKDNKEIAS ILNEMIQSDN
610 620 630 640 650
SSSDGKPLDS GLLHNNDRLS DGDSKYSQTS HKLVQLLTTT AEQQLRHADI
660 670 680 690 700
DTSCKDVLSC TGTSNSASAN SSGGSCPSSH SSLTERHKIL HRLLQEGSPS
710 720 730 740 750
DITTLSVEPD KKDSASTSVS VTGQVQGNSS IKLELDASKK KESKDHQLLR
760 770 780 790 800
YLLDKDEKDL RSTPNLSLDD VKVKVEKKEQ MDPCNTNPTP MTKPTPEEIK
810 820 830 840 850
LEAQSQFTAD LDQFDQLLPT LEKAAQLPGL CETDRMDGAV TSVTIKSEIL
860 870 880 890 900
PASLQSATAR PTSRLNRLPE LELEAIDNQF GQPGTGDQIP WTNNTVTAIN
910 920 930 940 950
QSKSEDQCIS SQLDELLCPP TTVEGRNDEK ALLEQLVSFL SGKDETELAE
960 970 980 990 1000
LDRALGIDKL VQGGGLDVLS ERFPPQQATP PLIMEERPNL YSQPYSSPSP
1010 1020 1030 1040 1050
TANLPSPFQG MVRQKPSLGT MPVQVTPPRG AFSPGMGMQP RQTLNRPPAA
1060 1070 1080 1090 1100
PNQLRLQLQQ RLQGQQQLIH QNRQAILNQF AATAPVGINM RSGMQQQITP
1110 1120 1130 1140 1150
QPPLNAQMLA QRQRELYSQQ HRQRQLIQQQ RAMLMRQQSF GNNLPPSSGL
1160 1170 1180 1190 1200
PVQMGNPRLP QGAPQQFPYP PNYGTNPGTP PASTSPFSQL AANPEASLAN
1210 1220 1230 1240 1250
RNSMVSRGMT GNIGGQFGTG INPQMQQNVF QYPGAGMVPQ GEANFAPSLS
1260 1270 1280 1290 1300
PGSSMVPMPI PPPQSSLLQQ TPPASGYQSP DMKAWQQGAI GNNNVFSQAV
1310 1320 1330 1340 1350
QNQPTPAQPG VYNNMSITVS MAGGNTNVQN MNPMMAQMQM SSLQMPGMNT
1360 1370 1380 1390 1400
VCPEQINDPA LRHTGLYCNQ LSSTDLLKTE ADGTQQVQQV QVFADVQCTV
1410 1420 1430 1440 1441
NLVGGDPYLN QPGPLGTQKP TSGPQTPQAQ QKSLLQQLLT E

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1255 Collapse assay details

Data source: Panorama

Official Gene Symbol
NCOA1
Peptide Sequence
LVQGGGLDVLSER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
960
Peptide End
972
CPTAC ID
CPTAC-1255
Peptide Molecular Mass
1,341.7252
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR 		CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR 		CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK 		CPTAC-1223:
MGEA5.
EIPVESIEEVSK 		CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK 		CPTAC-1226:
VAV1.
LNPGDIVELTK 		CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK 		CPTAC-1229:
ADCY9.
TDAHFVDVIK 		CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK 		CPTAC-1232:
B2M.
VEHSDLSFSK 		CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK 		CPTAC-1235:
CALCOCO1.
LQLEGQVTELR 		CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR 		CPTAC-1238:
CDKN1B.
VSNGSPSLER 		CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR 		CPTAC-1241:
ESR1.
LLFAPNLLLDR 		CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR 		CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR 		CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR 		CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR 		CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK 		CPTAC-1250:
IL18.
SDIIFFQR 		CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK 		CPTAC-1253:
CDC25B.
LLGHSPVLR 		CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER 		CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK 		CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK 		CPTAC-1259:
NEK2.
YSDELNEIITR 		CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK 		CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR 		CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK 		CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR 		CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK 		CPTAC-1268:
SMAD4.
GFPHVIYAR 		CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK 		CPTAC-1271:
STAT5A.
HILYNEQR 		CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR 		CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR 		CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK 		CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 8.3 6.4 4.5 13.9 7.7 10.2 16.2 10 11.1 15 15 15
y6 (1+) 12.7 7 5.7 14.3 9.4 9.7 19.1 11.7 11.3 15 15 15
y9 (1+) 9.6 6.6 6.9 13.8 9.4 9.1 16.8 11.5 11.4 15 15 15
sum 6.8 5.6 4.9 11.1 8.1 9.5 13 9.8 10.7 15 15 15

Selectivity

Data source: Panorama

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Slope of Curve Fit for cell line
fragment_ion 1 2 3 4 5 6
y5 (1+) 1.69 1.83 1.91 1.6 1.78 1.7
y9 (1+) 1.63 1.67 1.69 1.52 1.67 1.68
y6 (1+) 1.64 1.71 1.79 1.5 1.7 1.74
sum 1.66 1.75 1.81 1.54 1.72 1.71

Stability

Data source: Panorama

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Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
y6 (1+) 5.3 6.2 3.9 3 4.4
y9 (1+) 3.2 5.5 3.1 1.8 4.1
y5 (1+) 2.1 1.4 5.9 4.5 5.7
sum 3.2 3.5 3.8 3.1 3.3
Fragment ion / Transition all_intra_CV all_inter_CV
y6 (1+) 4.8 5
y9 (1+) 3.8 3.4
y5 (1+) 3.5 5.3
sum 3.4 3.9

Endogenous

Data source: Panorama

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Fragment ion / Transition intra_CV inter_CV total_CV
y6 (1+) 51.8 46.8 69.8
y9 (1+) 42.7 52 67.3
y5 (1+) 40.4 43.2 59.1
sum 29.4 31.9 43.4

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
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