Overview Data source: UniProt

Official Gene Symbol Other Aliases
OGT N/A
Sequence Length (AA) Molecular Weight (Da)
1046 116925
Protein Name
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASSVGNVAD STEPTKRMLS FQGLAELAHR EYQAGDFEAA ERHCMQLWRQ
60 70 80 90 100
EPDNTGVLLL LSSIHFQCRR LDRSAHFSTL AIKQNPLLAE AYSNLGNVYK
110 120 130 140 150
ERGQLQEAIE HYRHALRLKP DFIDGYINLA AALVAAGDME GAVQAYVSAL
160 170 180 190 200
QYNPDLYCVR SDLGNLLKAL GRLEEAKACY LKAIETQPNF AVAWSNLGCV
210 220 230 240 250
FNAQGEIWLA IHHFEKAVTL DPNFLDAYIN LGNVLKEARI FDRAVAAYLR
260 270 280 290 300
ALSLSPNHAV VHGNLACVYY EQGLIDLAID TYRRAIELQP HFPDAYCNLA
310 320 330 340 350
NALKEKGSVA EAEDCYNTAL RLCPTHADSL NNLANIKREQ GNIEEAVRLY
360 370 380 390 400
RKALEVFPEF AAAHSNLASV LQQQGKLQEA LMHYKEAIRI SPTFADAYSN
410 420 430 440 450
MGNTLKEMQD VQGALQCYTR AIQINPAFAD AHSNLASIHK DSGNIPEAIA
460 470 480 490 500
SYRTALKLKP DFPDAYCNLA HCLQIVCDWT DYDERMKKLV SIVADQLEKN
510 520 530 540 550
RLPSVHPHHS MLYPLSHGFR KAIAERHGNL CLDKINVLHK PPYEHPKDLK
560 570 580 590 600
LSDGRLRVGY VSSDFGNHPT SHLMQSIPGM HNPDKFEVFC YALSPDDGTN
610 620 630 640 650
FRVKVMAEAN HFIDLSQIPC NGKAADRIHQ DGIHILVNMN GYTKGARNEL
660 670 680 690 700
FALRPAPIQA MWLGYPGTSG ALFMDYIITD QETSPAEVAE QYSEKLAYMP
710 720 730 740 750
HTFFIGDHAN MFPHLKKKAV IDFKSNGHIY DNRIVLNGID LKAFLDSLPD
760 770 780 790 800
VKIVKMKCPD GGDNADSSNT ALNMPVIPMN TIAEAVIEMI NRGQIQITIN
810 820 830 840 850
GFSISNGLAT TQINNKAATG EEVPRTIIVT TRSQYGLPED AIVYCNFNQL
860 870 880 890 900
YKIDPSTLQM WANILKRVPN SVLWLLRFPA VGEPNIQQYA QNMGLPQNRI
910 920 930 940 950
IFSPVAPKEE HVRRGQLADV CLDTPLCNGH TTGMDVLWAG TPMVTMPGET
960 970 980 990 1000
LASRVAASQL TCLGCLELIA KNRQEYEDIA VKLGTDLEYL KKVRGKVWKQ
1010 1020 1030 1040 1046
RISSPLFNTK QYTMELERLY LQMWEHYAAG NKPDHMIKPV EVTESA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Assay Details for CPTAC-1261 Collapse assay details

Data source: Panorama

Official Gene Symbol
OGT
Peptide Sequence
AFLDSLPDVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
743
Peptide End
752
CPTAC ID
CPTAC-1261
Peptide Molecular Mass
1,103.5863
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR
CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR
CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK
CPTAC-1223:
MGEA5.
EIPVESIEEVSK
CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK
CPTAC-1226:
VAV1.
LNPGDIVELTK
CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK
CPTAC-1229:
ADCY9.
TDAHFVDVIK
CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK
CPTAC-1232:
B2M.
VEHSDLSFSK
CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK
CPTAC-1235:
CALCOCO1.
LQLEGQVTELR
CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR
CPTAC-1238:
CDKN1B.
VSNGSPSLER
CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR
CPTAC-1241:
ESR1.
LLFAPNLLLDR
CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR
CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR
CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR
CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR
CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1250:
IL18.
SDIIFFQR
CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK
CPTAC-1253:
CDC25B.
LLGHSPVLR
CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER
CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK
CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK
CPTAC-1259:
NEK2.
YSDELNEIITR
CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK
CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR
CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK
CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR
CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK
CPTAC-1268:
SMAD4.
GFPHVIYAR
CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK
CPTAC-1271:
STAT5A.
HILYNEQR
CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR
CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR
CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK
CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 15.3 3.6 7.3 24.7 8.4 9.7 29.1 9.1 12.1 15 15 15
y7 (1+) 13.2 4 5.9 15.4 5.8 11.3 20.3 7 12.7 15 15 15
y8 (1+) 15.3 4.1 5.1 14.5 5.8 11.2 21.1 7.1 12.3 15 15 15
sum 9.7 3.4 5 11.6 5.8 10.5 15.1 6.7 11.6 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1262 Collapse assay details

Data source: Panorama

Official Gene Symbol
OGT
Peptide Modified Sequence
GSVAEAEDC[+57.0]YNTALR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
315
Peptide - Site of Modification
9
Peptide Start
307
Peptide End
321
CPTAC ID
CPTAC-1262
Peptide Molecular Mass
1,654.7257
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR
CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR
CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK
CPTAC-1223:
MGEA5.
EIPVESIEEVSK
CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK
CPTAC-1226:
VAV1.
LNPGDIVELTK
CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK
CPTAC-1229:
ADCY9.
TDAHFVDVIK
CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK
CPTAC-1232:
B2M.
VEHSDLSFSK
CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK
CPTAC-1235:
CALCOCO1.
LQLEGQVTELR
CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR
CPTAC-1238:
CDKN1B.
VSNGSPSLER
CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR
CPTAC-1241:
ESR1.
LLFAPNLLLDR
CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR
CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR
CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR
CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR
CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1250:
IL18.
SDIIFFQR
CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK
CPTAC-1253:
CDC25B.
LLGHSPVLR
CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER
CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK
CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK
CPTAC-1259:
NEK2.
YSDELNEIITR
CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK
CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR
CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK
CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR
CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK
CPTAC-1268:
SMAD4.
GFPHVIYAR
CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK
CPTAC-1271:
STAT5A.
HILYNEQR
CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR
CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR
CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK
CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 19.5 7.7 8.6 21 9.8 21.2 28.7 12.5 22.9 15 15 15
y8 (1+) 13.2 4.8 5.4 16.2 10.8 13.9 20.9 11.8 14.9 15 15 15
y9 (1+) 14 5.1 4.7 15.2 8.2 12.7 20.7 9.7 13.5 15 15 15
sum 7.3 4.4 4.6 12.9 9.2 14.8 14.8 10.2 15.5 15 15 15

Additional Resources and Comments