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Overview Data source: UniProt

Official Gene Symbol Other Aliases
B2M N/A
Sequence Length (AA) Molecular Weight (Da)
119 13715
Protein Name
Beta-2-microglobulin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSRSVALAVL ALLSLSGLEA IQRTPKIQVY SRHPAENGKS NFLNCYVSGF
60 70 80 90 100
HPSDIEVDLL KNGERIEKVE HSDLSFSKDW SFYLLYYTEF TPTEKDEYAC
110 119
RVNHVTLSQP KIVKWDRDM

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1232 Collapse assay details

Data source: Panorama

Official Gene Symbol
B2M
Peptide Sequence
VEHSDLSFSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
69
Peptide End
78
CPTAC ID
CPTAC-1232
Peptide Molecular Mass
1,147.5510
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR
CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR
CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK
CPTAC-1223:
MGEA5.
EIPVESIEEVSK
CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK
CPTAC-1226:
VAV1.
LNPGDIVELTK
CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK
CPTAC-1229:
ADCY9.
TDAHFVDVIK
CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK
CPTAC-1232:
B2M.
VEHSDLSFSK
CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK
CPTAC-1235:
CALCOCO1.
LQLEGQVTELR
CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR
CPTAC-1238:
CDKN1B.
VSNGSPSLER
CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR
CPTAC-1241:
ESR1.
LLFAPNLLLDR
CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR
CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR
CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR
CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR
CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1250:
IL18.
SDIIFFQR
CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK
CPTAC-1253:
CDC25B.
LLGHSPVLR
CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER
CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK
CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK
CPTAC-1259:
NEK2.
YSDELNEIITR
CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK
CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR
CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK
CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR
CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK
CPTAC-1268:
SMAD4.
GFPHVIYAR
CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK
CPTAC-1271:
STAT5A.
HILYNEQR
CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR
CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR
CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK
CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 21.9 5.7 9.2 22.1 9.5 12.8 31.1 11.1 15.8 15 15 15
y5 (1+) 29.4 7.9 15.2 32.5 8.7 17.5 43.8 11.8 23.2 15 15 15
y7 (1+) 36.9 14.8 9.2 34.7 16 10.5 50.7 21.8 14 15 15 15
sum 16 6.8 9.7 16 9.9 12.3 22.6 12 15.7 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3965 Collapse assay details

Data source: Panorama

Official Gene Symbol
B2m
Peptide Sequence
TPQIQVYSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
24
Peptide End
32
CPTAC ID
non-CPTAC-3965
Peptide Molecular Mass
1,090.5771
Species
Mus musculus (Mouse)
Assay Type
Direct MRM or SRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

CPTAC-1219:
AURKB.
LPLAQVSAHPWVR
CPTAC-1220:
CHUK.
LGTGGFGNVC[+57.0]LYQHR
CPTAC-1221:
CHUK.
VWAEAVHYVSGLK
CPTAC-1222:
JUN.
NSDLLTSPDVGLLK
CPTAC-1223:
MGEA5.
EIPVESIEEVSK
CPTAC-1224:
PCNA.
DLSHIGDAVVISC[+57.0]AK
CPTAC-1225:
PCNA.
FSASGELGNGNIK
CPTAC-1226:
VAV1.
LNPGDIVELTK
CPTAC-1227:
VAV1.
YC[+57.0]SQVESASK
CPTAC-1228:
AURKB.
SNVQPTAAPGQK
CPTAC-1229:
ADCY9.
TDAHFVDVIK
CPTAC-1230:
ADCY9.
VIPQHQLSISPDIR
CPTAC-1231:
ARHGEF1.
ELVPPDTLHSLPK
CPTAC-1232:
B2M.
VEHSDLSFSK
CPTAC-1233:
B3GNT8.
NLLLVRPLGPQASIR
CPTAC-1234:
CALCOCO1.
GAQELAASSQQK
CPTAC-1235:
CALCOCO1.
LQLEGQVTELR
CPTAC-1236:
CCNB1.
TALGDIGNK
CPTAC-1237:
CDKN1B.
NLFGPVDHEELTR
CPTAC-1238:
CDKN1B.
VSNGSPSLER
CPTAC-1239:
C3.
TIYTPGSTVLYR
CPTAC-1240:
EP300.
LGTFLENR
CPTAC-1241:
ESR1.
LLFAPNLLLDR
CPTAC-1242:
FOXM1.
VLLAEEGIAPLSSAGPGK
CPTAC-1243:
GNAI1.
DSGVQAC[+57.0]FNR
CPTAC-1244:
GNAI1.
IAQPNYIPTQQDVLR
CPTAC-1245:
GNAI3.
IDFGEAAR
CPTAC-1246:
GNAI3.
ISQSNYIPTQQDVLR
CPTAC-1247:
GRB10.
SQQDPAGPGLPAQSDR
CPTAC-1248:
HDAC1.
YYAVNYPLR
CPTAC-1249:
IL18.
ISTLSC[+57.0]ENK
CPTAC-1250:
IL18.
SDIIFFQR
CPTAC-1251:
JAK1.
QLASALSYLEDK
CPTAC-1252:
JAK2.
LSDPGISITVLPK
CPTAC-1253:
CDC25B.
LLGHSPVLR
CPTAC-1254:
CDC25B.
TAVNLPLER
CPTAC-1255:
NCOA1.
LVQGGGLDVLSER
CPTAC-1256:
NCOA3.
AVSLDSPVSVGSSPPVK
CPTAC-1257:
NCOA3.
LLQNGNSPAEVAK
CPTAC-1258:
NEK2.
SQDSSPVLSELK
CPTAC-1259:
NEK2.
YSDELNEIITR
CPTAC-1260:
NFKB1.
LSPAPSK
CPTAC-1261:
OGT.
AFLDSLPDVK
CPTAC-1262:
OGT.
GSVAEAEDC[+57.0]YNTALR
CPTAC-1263:
PCP4.
AAVAIQSQFR
CPTAC-1264:
PLK1.
HINPVAASLIQK
CPTAC-1265:
RANBP10.
LYPAVNQQETPLPR
CPTAC-1266:
RANBP10.
VQGTVHC[+57.0]FPISAR
CPTAC-1267:
SKP2.
LSDPIVNTLAK
CPTAC-1268:
SMAD4.
GFPHVIYAR
CPTAC-1269:
SMAD4.
YC[+57.0]QYAFDLK
CPTAC-1270:
STAT5A.
ATIISEQQAK
CPTAC-1271:
STAT5A.
HILYNEQR
CPTAC-1272:
STAT1.
NLSFFLTPPC[+57.0]AR
CPTAC-1273:
STAT2.
EVLQSLPLTEIIR
CPTAC-1274:
STAT2.
HYQLLTEENIPENPLR
CPTAC-1275:
STAT3.
FPELNYQLK
CPTAC-1276:
STAT3.
GLSIEQLTTLAEK
CPTAC-1277:
STAT4.
YLYPDIPK

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 12.3 7.3 5.9 15.1 12.5 7.9 19.5 14.5 9.9 15 15 15
y7 (1+) 10.6 8.5 3.2 10.5 10.6 5.7 14.9 13.6 6.5 15 15 15
y8 (2+) 10.3 5.3 5.7 9.6 5.6 6.6 14.1 7.7 8.7 15 15 15
y4 (1+) 21.7 6.6 3.9 27.2 7.1 5.3 34.8 9.7 6.6 15 15 15
y5 (1+) 12.4 4.7 4.6 13.5 6 5.6 18.3 7.6 7.2 15 15 15
sum 3.6 3.2 2.9 5.6 5 4.1 6.7 5.9 5 15 15 15

Additional Resources and Comments