B2m, CPTAC-3965 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
B2m	N/A

Sequence Length (AA)	Molecular Weight (Da)
119	13779

Protein Name
Beta-2-microglobulin

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MARSVTLVFL	VLVSLTGLYA	IQKTPQIQVY	SRHPPENGKP	NILNCYVTQF
60	70	80	90	100
HPPHIEIQML	KNGKKIPKVE	MSDMSFSKDW	SFYILAHTEF	TPTETDTYAC
110	119
RVKHASMAEP	KTVYWDRDM

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1232 Collapse assay details

Data source: Panorama

Official Gene Symbol: B2M
Peptide Sequence: VEHSDLSFSK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 69
Peptide End: 78

CPTAC ID: CPTAC-1232
Peptide Molecular Mass: 1,147.5510
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	21.9	5.7	9.2	22.1	9.5	12.8	31.1	11.1	15.8	15	15	15
y5 (1+)	29.4	7.9	15.2	32.5	8.7	17.5	43.8	11.8	23.2	15	15	15
y7 (1+)	36.9	14.8	9.2	34.7	16	10.5	50.7	21.8	14	15	15	15
sum	16	6.8	9.7	16	9.9	12.3	22.6	12	15.7	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3965 Collapse assay details

Data source: Panorama

Official Gene Symbol: B2m
Peptide Sequence: TPQIQVYSR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 24
Peptide End: 32

CPTAC ID: non-CPTAC-3965
Peptide Molecular Mass: 1,090.5771
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

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Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.