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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Atf6b

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Atf6b Crebl1
Sequence Length (AA) Molecular Weight (Da)
699 76007
Protein Name
Cyclic AMP-dependent transcription factor ATF-6 beta
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAELMLLSEI ADPTRFFTDN LLSPEDWDST LYSGLDEVAE EQAQLFRCVE
60 70 80 90 100
QDVPFDSSSL DVGMDVSPPE PPWDPLPIFP DLQVKSEPSS PCSSSSLSSE
110 120 130 140 150
SSHLSTEPPS QVPGVGEVLH VKMESLAPPL CLLGDDPASP FETVQITVGS
160 170 180 190 200
ASDDLSDIQT KLEPASPSSS VHSEASLLSA DSPSQPFIGE EVLEVKTESP
210 220 230 240 250
SPPGCLLWDV PASSLGAVQI SMGPSPDSSS GKAPATRKPP LQPKPVVLTT
260 270 280 290 300
VPVPPRAGPT SAAVLLQPLV QQPAVSPVVL IQGAIRVQPE GPAPAAPRPE
310 320 330 340 350
RKSIVPAPMP GNSCPPEVDA KLLKRQQRMI KNRESACQSR RKKKEYLQGL
360 370 380 390 400
EARLQAVLAD NQQLRRENAA LRRRLEALLA ENSGLKLGSG NRKVVCIMVF
410 420 430 440 450
LLFIAFNFGP VSISEPPPAP MSPRMSREEP RPQRHLLGFS EPGPAHGMEP
460 470 480 490 500
LREAAQSPGE QQPSSAGRPS FRNLTAFPGG AKELLLRDLD QLFLSSDCRH
510 520 530 540 550
FNRTESLRLA DELSGWVQRH QRGRRKIPHR AQERQKSQLR KKSPPVKPVP
560 570 580 590 600
TQPPGPPERD PVGQLQLYRH PGRSQPEFLD AIDRREDTFY VVSFRRDHLL
610 620 630 640 650
LPAISHNKTS RPKMSLVMPA MAPNETVSGR GPPGDYEEMM QIECEVMDTR
660 670 680 690 699
VIHIKTSTVP PSLRKQPSPS PGNTTGGPLP GSAASPAHQA SQPLYLNHP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3964 Collapse assay details

Data source: Panorama

Official Gene Symbol
Atf6b
Peptide Sequence
LEALLAENSGLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
375
Peptide End
386
CPTAC ID
non-CPTAC-3964
Peptide Molecular Mass
1,256.6976
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 6.5 2.2 2.1 11.6 4.2 3.4 13.3 4.7 4 15 15 15
b2 (1+) 12.5 3 2.4 20 6.2 5.1 23.6 6.9 5.6 15 15 15
y7 (1+) 10.3 2.3 3.4 11.2 5.2 5.6 15.2 5.7 6.6 15 15 15
y8 (1+) 8.4 4 2.1 10 4.6 2.4 13.1 6.1 3.2 15 15 15
y10 (1+) 9.6 3.9 3.1 15.5 4.5 4.2 18.2 6 5.2 15 15 15
sum 3.5 1.3 1.3 9.5 3.8 3.4 10.1 4 3.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments