Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Adamts20

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Adamts20 N/A
Sequence Length (AA) Molecular Weight (Da)
1906 212068
Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 20
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRVAKWLTGL LCPISLLLTG SWEVRFHPRQ EALVKTLASY EVVTPTRVNE
60 70 80 90 100
FGDVFPQNRH FSRKKRSSGV PEPPPFRTHY RISAYGQLFQ LNLSADAAFL
110 120 130 140 150
AAGYTEVHLG TPVPGPGGRS TESPDLRHCF YRGQVNARED HTAVFSLCGG
160 170 180 190 200
LMGTFKANDG EYFLEPVLRA DGSAHDDDHN KPHLIYRQEL KRNSFARSHK
210 220 230 240 250
PCEVSENQME KTALPSQSSR NTTGDVDIEE EAVFRLEGER SQLHSRNKRF
260 270 280 290 300
LSYPRYVEVM VTADAKMVHH HGQNLQHYVL TLMSIVAAIY KDSSIGNLIN
310 320 330 340 350
IVIVKLVVIH SEQEGPVISF NAATTLRNFC LWQQSQNVPD DAHPSHHDTA
360 370 380 390 400
VLITREDICG AKEKCDTLGL AELGTLCDPS RSCSISEENG LSAAFTIAHE
410 420 430 440 450
LGHVFNVPHD DSFKCKEAGI KHQYHVMAPT LNYHTSPWTW SACSQKHITE
460 470 480 490 500
FLDTGHGECL LDKPNGRTYD LSPQLPGSVY DGNRQCELMF GPGSQVCPYL
510 520 530 540 550
KHCRRLWCTS AEGVHKGCRT QHMPLADGTS CGPGMHCHRG LCVTRDMETR
560 570 580 590 600
PVDGEWGPWG PYSSCSRTCG GGIKSTARLC DRPEPRNGGR YCVGRRMKFR
610 620 630 640 650
SCNTDSCPKG KRDFREKQCS DFDGKHFDIN GLPPNVRWLP KYSGIAVKDR
660 670 680 690 700
CKLYCRVAGT TSFYQLKDRV ADGTPCGTET NDICVQGLCR QAGCDHVLNS
710 720 730 740 750
KAKRDKCGVC GGDNSSCQTL AGVFNSAHYG YNVVVKIPAG ATNIEILQHS
760 770 780 790 800
YSGRPEDDNY LALSDTQGNF LLNGNFVVSM AKKEINIQGA VFEYSGSNNS
810 820 830 840 850
IERINSTDRL EAELVLQVLC VGNLYNPDVR YSFNIPIEER SNLFSWDPYG
860 870 880 890 900
PWQDCTKMCQ GLHRRKIACV RKSDHAVVSD HNCGHLPMPL FVTEKCNMDC
910 920 930 940 950
ELRWHIIGKS DCSSQCGQGY RTLDVHCMKY SVHKGQAVPV GDQYCGDQLK
960 970 980 990 1000
PPSREPCHGS CVLTRWHYSE WSQCSRSCGG GDKTRESYCV NGFGHRLAES
1010 1020 1030 1040 1050
ECRELPRVVL ENCNEFPCPS WATSEWSECP VTCGKGMKQR QVWCQLSEDP
1060 1070 1080 1090 1100
MRDGFCNAST KPESLRPCEL RACASWHVGP WGSCTATCGH GYQMRAVKCI
1110 1120 1130 1140 1150
SEIFGTMLDD RECPQASRPS DRQDCILAPC LAIPEVGATS LPAIPLGRAA
1160 1170 1180 1190 1200
QWRHGSWTPC SVSCGRGSQA RYVSCRDAHD EVADESNCAH LPRPAAVSLC
1210 1220 1230 1240 1250
FSPCGEWQAG DWSPCSASCG HGKTTRRVLC VNYHQLVDES YCDPEGRPVT
1260 1270 1280 1290 1300
EQECSLAACP PLYSRAPSSS EQPSHVPSRN VPLTHKPGEN QDQGAQLSIR
1310 1320 1330 1340 1350
GNQWRTGPWG ACSRSCAGGL QHRAVVCQDE DGRSATSCDG SSKPPESRHC
1360 1370 1380 1390 1400
GSGPCPHWNY GDWGECTQTC GGGVKSRFVI CQFPNGQMTQ EHSCELPKPP
1410 1420 1430 1440 1450
SMMQCHLHAC PEDVSWYRGP WKSCSASCGK GVKYREVLCI DQFQRKLEEK
1460 1470 1480 1490 1500
YCSHLHKPRT HKACRSGRCP SWKANKWKEC SVTCGSGVQQ REVYCRLRGT
1510 1520 1530 1540 1550
GRVSEDMCDP STRPQGQRQC WRQDCMRYQW TTGDWLDCST SCKKKETYRL
1560 1570 1580 1590 1600
VKCVNEQNVQ ANESLCDPLT KPLSIKKCRN PHCKYSVVTG DSSQCAGNCG
1610 1620 1630 1640 1650
FTSPQKITYC TKIQSSKKHT FHQLRPVVYG ECPVIPSPQA YKCDLRSCLH
1660 1670 1680 1690 1700
VATWKVGKWS KCSVTCGIGI MERRVACRTE NGWPSDLCLK RLKPDAQKKC
1710 1720 1730 1740 1750
YANDCKLLTT CKELQVTNNV TKDGDYDLNV RGRILKIHCS GMQLENPREY
1760 1770 1780 1790 1800
LPLVKSEDNF SEIYGLRLQN PYECPFNGSR RPDCACENDY LPAGYTVFSK
1810 1820 1830 1840 1850
VRVDLESMQI KTADLLFSQT LSGKAVPFAT AGDCYSAARC PQGQFSINLA
1860 1870 1880 1890 1900
GTGMKISNTA KWLAQGRYAS VIIHRSQDGT KVYGRCGGFC GKCIPHMATG
1906
LSIQVL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-3963 Collapse assay details

Data source: Panorama

Official Gene Symbol
Adamts20
Peptide Sequence
LYNPDVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
824
Peptide End
830
CPTAC ID
non-CPTAC-3963
Peptide Molecular Mass
875.4501
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 37 5.8 6.5 34.2 7.8 6.8 50.4 9.7 9.4 15 15 15
b2 (1+) 9.5 6 4.6 11.1 6.7 4.5 14.6 9 6.4 15 15 15
y2 (1+) 18.8 5 6.7 17.1 7.6 7.7 25.4 9.1 10.2 15 15 15
y4 (1+) 13.7 4.2 4.7 13.8 4.4 5.8 19.4 6.1 7.5 15 15 15
y5 (1+) 6.9 6.8 3.2 11.7 6.7 5.5 13.6 9.5 6.4 15 15 15
sum 7.6 2.7 1.2 7.8 4.5 3.9 10.9 5.2 4.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments