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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Adamts1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Adamts1 N/A
Sequence Length (AA) Molecular Weight (Da)
968 105801
Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQPKVPLGSR KQKPCSDMGD VQRAARSRGS LSAHMLLLLL ASITMLLCAR
60 70 80 90 100
GAHGRPTEED EELVLPSLER APGHDSTTTR LRLDAFGQQL HLKLQPDSGF
110 120 130 140 150
LAPGFTLQTV GRSPGSEAQH LDPTGDLAHC FYSGTVNGDP GSAAALSLCE
160 170 180 190 200
GVRGAFYLQG EEFFIQPAPG VATERLAPAV PEEESSARPQ FHILRRRRRG
210 220 230 240 250
SGGAKCGVMD DETLPTSDSR PESQNTRNQW PVRDPTPQDA GKPSGPGSIR
260 270 280 290 300
KKRFVSSPRY VETMLVADQS MADFHGSGLK HYLLTLFSVA ARFYKHPSIR
310 320 330 340 350
NSISLVVVKI LVIYEEQKGP EVTSNAALTL RNFCSWQKQH NSPSDRDPEH
360 370 380 390 400
YDTAILFTRQ DLCGSHTCDT LGMADVGTVC DPSRSCSVIE DDGLQAAFTT
410 420 430 440 450
AHELGHVFNM PHDDAKHCAS LNGVSGDSHL MASMLSSLDH SQPWSPCSAY
460 470 480 490 500
MVTSFLDNGH GECLMDKPQN PIKLPSDLPG TLYDANRQCQ FTFGEESKHC
510 520 530 540 550
PDAASTCTTL WCTGTSGGLL VCQTKHFPWA DGTSCGEGKW CVSGKCVNKT
560 570 580 590 600
DMKHFATPVH GSWGPWGPWG DCSRTCGGGV QYTMRECDNP VPKNGGKYCE
610 620 630 640 650
GKRVRYRSCN IEDCPDNNGK TFREEQCEAH NEFSKASFGN EPTVEWTPKY
660 670 680 690 700
AGVSPKDRCK LTCEAKGIGY FFVLQPKVVD GTPCSPDSTS VCVQGQCVKA
710 720 730 740 750
GCDRIIDSKK KFDKCGVCGG NGSTCKKMSG IVTSTRPGYH DIVTIPAGAT
760 770 780 790 800
NIEVKHRNQR GSRNNGSFLA IRAADGTYIL NGNFTLSTLE QDLTYKGTVL
810 820 830 840 850
RYSGSSAALE RIRSFSPLKE PLTIQVLMVG HALRPKIKFT YFMKKKTESF
860 870 880 890 900
NAIPTFSEWV IEEWGECSKT CGSGWQRRVV QCRDINGHPA SECAKEVKPA
910 920 930 940 950
STRPCADLPC PHWQVGDWSP CSKTCGKGYK KRTLKCVSHD GGVLSNESCD
960 968
PLKKPKHYID FCTLTQCS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3962 Collapse assay details

Data source: Panorama

Official Gene Symbol
Adamts1
Peptide Sequence
NSISLVVVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
301
Peptide End
309
CPTAC ID
non-CPTAC-3962
Peptide Molecular Mass
957.5859
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 9.6 4.7 3.8 9.8 5.8 3.9 13.7 7.5 5.4 15 15 15
y2 (1+) 11.1 6.5 6 17.4 7.4 8.2 20.6 9.8 10.2 15 15 15
y3 (1+) 28.3 5.8 12 37.3 13.4 32.6 46.8 14.6 34.7 15 15 15
y6 (1+) 8.5 4.4 4.2 12.4 6.3 6.6 15 7.7 7.8 15 15 15
y7 (1+) 9.4 3.6 2.3 14.2 7.2 6.1 17 8 6.5 15 15 15
sum 5.7 2.8 1.8 10.3 5.5 5.2 11.8 6.2 5.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments