KLK3, CPTAC-1476 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
KLK3	APS

Sequence Length (AA)	Molecular Weight (Da)
261	28741

Protein Name
Prostate-specific antigen

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MWVPVVFLTL	SVTWIGAAPL	ILSRIVGGWE	CEKHSQPWQV	LVASRGRAVC
60	70	80	90	100
GGVLVHPQWV	LTAAHCIRNK	SVILLGRHSL	FHPEDTGQVF	QVSHSFPHPL
110	120	130	140	150
YDMSLLKNRF	LRPGDDSSHD	LMLLRLSEPA	ELTDAVKVMD	LPTQEPALGT
160	170	180	190	200
TCYASGWGSI	EPEEFLTPKK	LQCVDLHVIS	NDVCAQVHPQ	KVTKFMLCAG
210	220	230	240	250
RWTGGKSTCS	GDSGGPLVCN	GVLQGITSWG	SEPCALPERP	SLYTKVVHYR
260	261
KWIKDTIVAN	P

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1476 Collapse assay details

Data source: Panorama

Official Gene Symbol: KLK3
Peptide Modified Sequence: IVGGWEC[+57.0]EK
Modification Type: Carbamidomethyl Cysteine
Protein - Site of Modification: 31
Peptide - Site of Modification: 7
Peptide Start: 25
Peptide End: 33

CPTAC ID: CPTAC-1476
Peptide Molecular Mass: 1,076.4961
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: depleted digested plasma
Submitting Laboratory: Broad Institute
Submitting Lab PI: Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Quantiva TSQ
Internal Standard: heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity: >95%
Peptide Standard Label Type: 15N

LC: Easy NanoLC1000
Column Packing: Reprosil C18, 1.9um, 200A
Column Dimensions: 0.075 x 150 mm
Flow Rate: 200 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	7.4	2.2	2.5	9.3	5.5	3.7	11.9	5.9	4.5	15	15	15
y7 (1+)	3.3	1.7	1.2	3.2	5.8	4.2	4.6	6	4.4	15	15	15
y3 (1+)	3.8	2.9	1.9	5.1	5.9	4.8	6.4	6.6	5.2	15	15	15
sum	2.7	1.8	1.3	3.2	5.7	4.2	4.2	6	4.4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-1296 Collapse assay details

Data source: Panorama

Official Gene Symbol: KLK3
Peptide Sequence: FLRPGDDSSHDLMLLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 110
Peptide End: 125

CPTAC ID: CPTAC-1296
Peptide Molecular Mass: 1,870.9360
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human serum digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang